Institute of Experimental Medicine CAS

We study the lateral organization of biological membranes into functional microdomains with the emphasis on their fine structure, dynamics and molecular principles of formation. Morphological changes or disintegration of these cellular structures are usually accompanied by the pathologic phenotypes. Taking the maximum advantage from the genetically accessible yeast model, the research at the Microscopy Unit is focused on the involvement of membrane microdomains in the stress perception and adaptation, signaling and regulation of metabolic processes. Recently we described the model of general membrane structure re-organization in response to membrane depolarization as induced by environmental stimuli, pathogen or stress exposure.

Deputy Head:

Petra Veselá, MSc
E-mail: petra.vesela@iem.cas.cz
Tel.: +420 241 062 119

Research Scientists:

Assoc. Prof. Jan Malínský, PhD.
Jakub Zahumenský, MSc, PhD.

Research Assistant:

Petra Veselá, MSc

PhD. Students:

Thuraya Awadová, MSc (ML)
Katarína Vaškovičová, MSc

Undergraduate Student:

Lenka Doudová

Technicans:

Jitka Eisensteinová
Dagmar Folková, MSc
Lenka Hlavínová (ML) 

Technican of microscopy:

Martin Oškera, MSc

 

Regulation of mRNA decay at the plasma membrane

  a

Evolutionarily conserved 5´-3´exoribonuclease Xrn1 binds to eisosome under conditions of glucose deprivation. Association of Xrn1 with the plasma membrane represents a novel mechanism of the mRNA decay regulation. Subcellular Xrn1 localization also reflects the nutrient supply and metabolic differentiation within the yeast colony. Central section of the whole 5 days old colony of S. cerevisiae expressing Xrn1-GFP (A), stratification of specific Xrn1-GFP patterns (B), and large magnification details (C) of cells showing Xrn1 distributed in cytosol (1), accumulated in P-bodies (2) and associated with eisosome (3) are presented. Fluorescence Recovery After Photobleaching  (FRAP) analysis revealed that in contrast to P-body binding, the enzyme association with the plasma membrane at eisosome is stable in time (D). Bars: 300 μm (A), 10 μm (C).

 

b
c
 
d

 

Publication:
Vaškovičová, K., Awadová, T., Veselá, P. , Balážová, M., Opekarová, M., Malínský, J.: (2017) mRNA decay is regulated via the spatial sequestration of the conserved 5 '-3 ' exoribonuclease Xrn1 at a specific microdomain of the yeast plasma membrane. European Journal of Cell Biology. 96(6): 591-599. IF 3,712.

 

2017

Vaškovičová, K., Awadová, T., Veselá, P. , Balážová, M., Opekarová, M., Malínský, J.: (2017) mRNA decay is regulated via the spatial sequestration of the conserved 5 '-3 ' exoribonuclease Xrn1 at a specific microdomain of the yeast plasma membrane. European Journal of Cell Biology. 96(6): 591-599. 

2016

Chum, T., Glatzová, D., Kvíčalová, Z., Malínský, J., Brdička, T., Cebecauer, M.: (2016) The role of palmitoylation and transmembrane domain in sorting of transmembrane adaptor proteins. J. Cell Sci., 129(1): 95-107.

Malínský, J., Opekarová, M.: (2016) New Insight Into the Roles of Membrane Microdomains in Physiological Activities of Fungal Cells. Int. Rev. Cell Mol. Biol., 325: 119-180.

Malínský, J., Tanner, W., Opekarová, M.: (2016) Transmembrane voltage: Potential to induce lateral microdomains. Acta Mol. Cell Biol. Lipids, 1861(8 Pt B): 806-811.

Plecitá-Hlavatá, L., Engstová, H., Alán, L., Špaček, T., Dlasková, A., Smolková, K., Špačková, J., Tauber, J., Strádalová, V., Malínský, J., Lessard, M., Bewersdorf, J., Ježek, P.: (2016) Hypoxic HepG2 cell adaptation decreases ATP synthase dimers and ATP production in inflated cristae by mitofilin down-regulation concomitant to MICOS clustering. Faseb J., 30(5): 1941-1957.

Pokorná, L., Čermáková, P., Horváth, A., Baile, M.G., Claypool, S.M., Griač, P., Malínský, J., Balážová, M.: (2016) Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function. Biochim. Biophys. Acta-Bioenerg., 1857(1): 34-45.

Wang, H.X., Douglas, L.M., Veselá, P., Rachel, R., Malinský, J., Konopka, J.B.: (2016) Eisosomes promote the ability of Sur7 to regulate plasma membrane organization in Candida albicans. Mol Biol Cell., 27(10): 1663-1675.

2015

Grousl, T., Opekarová, M., Strádalová, V., Hašek, J., Malínský, J.: (2015) Evolutionarily Conserved 5'-3' Exoribonuclease Xrn1 Accumulates at Plasma Membrane-Associated Eisosomes in Post-Diauxic Yeast. PLoS One. 10(3): e0122770.

Herman, P., Večeř, J., Opekarová, M., Veselá, P., Jančíková, I., Zahumenský, J., Malinský, J.: (2015) Depolarization affects lateral microdomain structure of yeast plasma membrane. FEBS J. 282(3): 419-434.

Pokorná, L., Čermáková, P., Horváth, A., Baile, M.G., Claypool, S.M., Griač, P., Malínský, J., Balážová, M.: (2015) Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function. Biochim Biophys Acta. 1857(1): 34-45.

Vaškovičová, K., Strádalová, V., Efenberk, A., Opekarová, M., Malínský, J.: (2015) Assembly of fission yeast eisosomes in the plasma membrane of budding yeast: Import of foreign membrane microdomains. Eur. J. Cell Biol. 94(1): 1-11.

2014

Vecer, J., Veselá, P., Malínský, J., Herman, P.: (2014) Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast. FEBS Lett. 588(3): 443-449.

2013

Malínský, J., Opekarová, M., Grossmann, G., Tanner, W.: (2013) Membrane microdomains, rafts, and detergent-resistant membranes in plants and fungi. Annu. Rev. Plant Biol. 64: 501-529.

Rinnerthaler, M., Slaba, R., Grousl, T., Strádalová, V., Heeren, G., Richter, K., Breitenbach-Koller, H., Malínský, J., Hasek, J., Breitenbach, M.: (2013) Mmi1, the yeast homologue of mammalian TCTP associates with stress granules in Heat-Shocked Cells and Modulates proteasome activity. PLoS One. 8(10): e77791.

 

SAV-18-25, Barth syndrome - Study of mitochondrial morphology in a novel yeast model, 2018-2020

Institute of Animal Biochemistry and Genetics SAS

University of Regensburg

Institute of Physics of Charles University

 Prague Membrane Discussions (PMD)

Informal scientific discussions focused on cellular membranes (not only).

  https://praguemembranediscussions.weebly.com/

pmd

 


 

The Summer Event will take place at IOCB in Dejvice (May or June). 

 


Spring PMD 2018

March 8th, 2018 ; 10 am - 2 pm
@ Jaroslav Heyrovsky Institute of the CAS, Dolejskova 3, Prague 8
Brdicka lecture room
 
Program:

10:00 - 11:00 Plenary lecture 1.
Claudia Steinem - Universitaet Goettingen, Germany
Pore-spanning membranes: Is this model system suited for addressing biochemical questions?

 
11:00 - 12:00 Plenary lecture 2.
Andreas Janshoff - Universitaet Goettingen, Germany
Membrane biophysics in confined geometry
 
12:00-12:30 COFFEE/SNACKS BREAK (drinks, coffee and snacks available)
 
12:30 – 13:15
Robert Vacha, MU Brno
Translocation of peptides across phospholipid membranes

13:15 – 14:00
Petra Riegrova, JHI
The oxidized phospholipids modify Bax protein ability to induce permeabilization of mitochondrial membranes

 



  2017

Poslední PMD v roce 2017 se uskutečnilo  ve čtvrtek 14. 12. a hostitelem byl tentokrát Ústav experimentální botaniky AV ČR.

konferenci-zahajil-doktor-jan-petrasek
Konferenci zahájil doktor Jan Petrášek.
vodni-prednasku-mel-charo-del-genio-z-britske-university-of-warwick
Úvodní přednášku měl Charo del Genio z britske University of Warwick.
krome-prednasek-bylo-na-akci-take-dost-casu-pro-neformalni-diskuse-a-setkani-s-kolegy

 ...kromě přednášek bylo na akci také dost času pro neformální diskuse a setkání s kolegy.

December 14th, 2017; 10 am - 3 pm
@ Institute of Experimental Botany of the CAS, Prague 6 - Lysolaje

Building B1, Ground floor, Library seminar room
 
Program:
10:00 - 10:30 Charo I. Del Genio
In silico modelling of auxin plasma membrane carrier
Centre for Complexity Science, University of Warvick, UK
 
10:30 - 11:00 Roman Pleskot
Membrane targeting of Arabidopsis TPLATE complex
Group of Advanced Live Cell Imaging, VIB Center for Plant Systems Biology, Ghent, Belgium
Roman Pleskot, Klaas Yperman, Jie Wang and Daniel Van Damme
 
11:00-11:20 COFFEE BREAK (drinks, coffee and snacks available)
 
11:20 – 11:50 Martin Potocký
Phosphatidic acid is involved in clathrin-mediated endocytosis in tip-growing plant cells
Laboratory of Cell Biology, Institute of Experimental Botany CAS
Přemysl Pejchar, Juraj Sekereš, Roman Pleskot, Viktor Žárský and Martin Potocký
 
11:50-12:20 Mária Balážová
Subcellular distribution of phosphatidylglycerol is regulated by a lipid droplet protein
Department of Membrane Biochemistry, Institute of Animal Biochemistry and Genetics, Centre of Biosciences SAS, Bratislava, Slovakia
 
12:20-13:30 Lunch - canteen of IEB CAS or pizza at the site
 
13:30 – 14:00 Radovan Fišer
Interaction of lipopeptide Daptomycin with the membrane and the pore-formation
Laboratory of Bacterial Physiology, Department of Genetics and Microbiology, Faculty of Science, Charles University
 
14:00 – 14:30 Michal Daněk
Flotillin2 in Arabidopsis or Do membrane microdomains reflect cell wall integrity?
Laboratory of Signal Transduction, Institute of Experimental Botany CAS
Michal Daněk, Jan Petrášek, Jindřiška Angelini, Kateřina Malínská
 
14:30-15:00 Katarzyna Retzer
SRRF based analysis of the distribution of a mutated variant of the plasma membrane located auxin carrier AtPIN2
Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany CAS

Mail room:
building La 2. floor, room 2.18
Mo–Fri   9:00–13:00

Data box:
kqcnc2p

Contacts

Vídeňská 1083
142 20 Praha 4-Krč
Czech Republic
+420 241 062 230