The Department of Neurochemistry studies molecular mechanisms that regulate the function of glutamate receptors in the mammalian central nervous system. We also aim to develop novel pharmacological compounds that act on glutamate receptors for the treatment of cognitive decline caused by neurodegeneration. Our experimental approach includes microscopy, biochemistry, and electrophysiology in cultured hippocampal neurones and human fibroblasts derived from patients with neurodegenerative diseases. Our long-term goal is to develop “personalised” compounds for treating specific neurodegenerative conditions including Alzheimer disease.
Deputy Head:
Oksana Forostyak, MD, PhD.
E-mail: oxfor@biomed.cas.cz
Research Scientists:
Oksana Forostyak, MD, PhD.
Martin Horák, PhD.
PhD. Students:
Marharyta Kolcheva, MSc
Štěpán Kortus, MSc
Katarína Lichnerová, MSc
Kristýna Skřenková, MSc
Technican:
Dominika Dušková
2017
Horak M, Benkovicova K, Krusek J, Kaniakova M, Korabecny J, Nepovimova E, Vyklicky L, Kuca K, Stuchlik A, Ricny J, Vales K, Soukup O. The pharmacology of tacrine at N-methyl-D-aspartate receptors. Progress in Neuropsychopharmacology & Biological Psychiatry. 75:54-62 (2017).
Gazova Z, Soukup O, Sepsova V, Siposova K, Drtinova L, Jost P, Spilovska K, Korabecny J, Nepovimova E, Fedunova D, Horak M, Kaniakova M, Wang ZJ, Hamouda AK, Kuca K. Multi-target-directed therapeutic potential of 7-methoxytacrine-adamantylamine heterodimers in the Alzheimer's disease treatment. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1863(2):607-619 (2017).
Bohuslavova R, Dodd N, Macova I, Chumak T, Horak M, Syka J, Fritzsch B, Pavlinkova G. Pax2-Islet1 Transgenic Mice Are Hyperactive and Have Altered Cerebellar Foliation. Molecular Neurobiology. 54(2):1352-1368 (2017).
2016
Kaniakova M, Lichnerova K, Skrenkova K, Vyklicky L, Horak M. Biochemical and electrophysiological characterization of N-glycans on NMDA receptor subunits. Journal of Neurochemistry. 138(4):546-56 (2016).
Vyklicky V, Smejkalova T, Krausova B, Balik A, Korinek M, Borovska J, Horak M, Chvojkova M, Kleteckova L, Vales K, Cerny J, Nekardova M, Chodounska H, Kudova E, Vyklicky L. Preferential Inhibition of Tonically over Phasically Activated NMDA Receptors by Pregnane Derivatives. Journal of Neuroscience. 36(7):2161-75 (2016).
2015
Lichnerova K, Kaniakova M, Park SP, Skrenkova K, Wang YX, Petralia RS, Suh YH, Horak M. Two N-glycosylation Sites in the GluN1 Subunit Are Essential for Releasing N-methyl-d-aspartate (NMDA) Receptors from the Endoplasmic Reticulum. Journal of Biological Chemistry. 290:18379-90 (2015).
Vyklicky V, Krausova B, Cerny J, Balik A, Zapotocky M, Novotny M, Lichnerova K, Smejkalova T, Kaniakova M, Korinek M, Petrovic M, Kacer P, Horak M, Chodounska H, Vyklicky L. Block of NMDA receptor channels by endogenous neurosteroids: implications for the agonist induced conformational states of the channel vestibule. Scientific Reports. 5:10935 (2015).
Korinek M, Vyklicky V, Borovska J, Lichnerova K, Kaniakova M, Krausova B, Krusek J, Balik A, Smejkalova T, Horak M, Vyklicky L. Cholesterol modulates open probability and desensitization of NMDA receptors. Journal of Physiology. 593:2279-93 (2015).
Horak M, Suh YH. Measuring the number of NMDA receptors at the cell surface. Chapter in Book: Ionotropic Glutamate Receptor Technologies, NeuroMethods, Springer, Series editor: Wolfgang Walz, Book editor: Gabriela K Popescu. (2015).
OP VVV PharmaBrain,
2018–2022
Regulation of the trafficking and function of GluN3A-containing NMDA receptors in mammalian neurones
GAČR, 18-04329S, 2018–2020
Role of transmembrane domain in transport and function of NMDA receptors
GAUK, 468217, 2017–2019
Novel hybrid compounds in the cognitive decline caused by neurodegeneration
GAČR, 16-08554S, 2016–2018
National Institute of Mental Health, Klecany
Biomedical Research Center, Hradec Kralove
Neuroscience Research Institute, Seoul National University College of Medicine, Republic of Korea
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