Institute of Experimental Medicine CAS

In our Department we investigate molecular characteristics of solid cancers, especially of the colon, rectum, pancreas and ovaries. Within these studies we focus on the molecular epidemiological level in order 1. to identify biomarkers of increased predisposition to tumour diseases, 2. enable early diagnostics, 3. assess individual responses to anti-tumour treatment, 4. and determine long-term prognosis. The Department has been working with different types of biological material from a patients with cancer diseases, such as solid tissue, blood cells or plasma.

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Deputy Head:

Veronika Vymetálková, MSc, PhD.
E-mail: vpolakova@biomed.cas.cz
Phone: +420 241 062 699

Research Scientists:

Alena Opattová, PhD.
Pavel Vodička, MD, PhD. 
Ludmila Vodičková, MD, PhD.
Veronika Vymetálková, MSc, PhD.

PhD. Students:

Linda Bártů, MSc
Petra Bendová, MSc
Klára Červená, MSc
Andrea Čumová, MSc
Kateřina Jirásková, MSc
Jan Král, MD
Michal Kroupa, MSc
Peter Mačinga, MD
Alexandra Rejhová, MSc
Soňa Vodenková, MSc

Phone: +420 241 062 251

Undergraduate Students:

Josef Horák
Alexandra Dolníková
Kristýna Tomášová

External Collaborators:

Dr. Alessio Naccarati 
Jana Slyšková, Ph.D. 
Markéta Urbanová, Ph.D.

Important results in 2018

1.  Analysis of the effect of functional genetic variants in DNA repair genes in relation to the risk of colorectal cancer

Results of the study of 16 genetic variants of DNA repair genes:

Identification of a single genetic polymorphism (rs3204953, the REV3L gene) associated with a 2.3-fold higher risk of disease for the carriers of variant genotype (p = 0.006) in association study.

Identification of variants rs3218649 in the gene POLQ and rs7689099 in the NEIL3 gene as significantly associated with overall survival (OS) and recurrent survival (RFS), using CART analysis.

  katka

 Publication: submitted to Carcinogenesis

 

Important results in 2017

1. MicroRNA-binding site polymorphisms in genes involved in colorectal cancer etiopathogenesis as a predictive biomarkers for treatment of the disease

The CC genotype in rs8679 in PARP gene was associated with an increased risk of recurrence/progression in pacient. Carriers of at least on C allele were at a decreased risk of cancer (P = 0.005). Moreover the CC genotype in rs8679 was also associated with an increased risk of recurrence/progression in patients that received 5-FU-based chemotherapy (log-rank test P = 0.03).

  parp

Kaplan–Meier EFS curves in CRC patients undergoing 5-FU-based chemotherapy stratified for rs8679 in PARP1 gene.

Publication:  Michaela Schneiderova, Alessio Naccarati, Barbara Pardini, Fabio Rosa, Cornelia Di Gaetano, Katerina Jiraskova, Alena Opattova, Miroslav Levy, Karel Veskrna, Veronika Veskrnova, Tomas Buchler, Stefano Landi, Pavel Vodicka, Veronika Vymetalkova; MicroRNA-binding site polymorphisms in genes involved in colorectal cancer etiopathogenesis and their impact on disease prognosis, Mutagenesis, Volume 32, Issue 5, 17 October 2017, Pages 533-542, https://doi.org/10.1093/mutage/gex026

 

2.The relative telomere length in peripheral lymphocytes may reflect the susceptibility to radiomimetics in colorectal cancer patient

We observed statistically significant increased level of chromosomal damage in CRC patients compared with controls after bleomycin treatment. Patients with shorter relative telomere length exhibited significantly higher amount of damage in peripheral lymphocytes after bleomycin treatment

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Spearman correlation of relative telomere length and chromosomal damage in CRC patients and controls.

Publication: Kroupa M , Polivkova Z, Rachakonda S, et al. Bleomycin-induced chromosomal damage and shortening of telomeres in peripheral blood lymphocytes of incident cancer patients. Genes Chromosomes Cancer. 2018;57:61–69. https://doi.org/10.1002/gcc.22508

 

Important results in 2016

1. Polymorphisms in microRNA binding sites of mucin genes are significantly associated with the risk of colorectal cancer development and patient's survival

Patients carrying the CC genotype of rs886403 in MUC21 displayed a shorter survival and higher recurrence risk when compared with TT.

1 2 3

Publication: Veronika Vymetalkova , Barbara Pardini, Fabio Rosa, Katerina Jiraskova, Cornelia Di Gaetano, Petra Bendova, Miloslav Levy, Veronika Veskrnova, Tomas Buchler, Ludmila Vodickova, Alessio Naccarati, Pavel Vodicka; Polymorphisms in microRNA binding sites of mucin genes as predictors of clinical outcome in colorectal cancer patients, Carcinogenesis, Volume 38, Issue 1, 1 January 2017, Pages 28–39, https://doi.org/10.1093/carcin/bgw114

 

2. DNA methylation profiles of selected genes as biomarkers in rectal carcinogenesis

Analysis of methylation profile of paired samples of patient with rectal carcinoma (tumor tussue vs. adjacent mucosa) revealed aberrant methylation in 33 genes. This study has demonstrated the possibility of using specific methylated DNA profiles as diagnostic biomarkers in patients with rectal carcinoma.

  metylace

Publication: Veronika Vymetalkova , Pavel Vodicka, Barbara Pardini, Fabio Rosa, Miroslav Levy, Michaela Schneiderova, Vaclav Liska, Ludmila Vodickova, Torbjörn K Nilsson, and Sanja A Farkas; Epigenome-wide analysis of DNA methylation reveals a rectal cancer-specific epigenomic signature, Epigenomics 2016 8:9, 1193-1207, https://doi.org/10.2217/epi-2016-0044

 

2018

Vodenkova S, Jiraskova K, Urbanova M, Kroupa M, Slyskova J, Schneiderova M, Levy M, Buchler T, Liska V, Vodickova L, Vymetalkova V, Collins A, Opattova A, Vodicka P. Base excision repair capacity as a determinant of prognosis and therapy response in colon cancer patients. DNA Repair (Amst). 2018 Oct 1. pii:S1568-7864(18)30070-3. doi: 10.1016/j.dnarep.2018.09.006.

Kral J , Korenkova V, Novosadova V, Langerova L, Schneiderova M, Liska V, Levy  M, Veskrnova V, Spicak J, Opattova A, Jiraskova K, Vymetalkova V, Vodicka P, Slyskova J. Expression profile of miR-17/92 cluster is predictive of treatment response in rectal cancer. Carcinogenesis. 2018 Oct 1. doi:10.1093/carcin/bgy100.

Thiele JA, Hosek P, Kralovcova E, Ostasov P, Liska V, Bruha J, Vycital O, Rosendorf J, Opattova A, Horak J, Kralickova M, Vodicka P, Pitule P. lncRNAs in Non-Malignant Tissue Have Prognostic Value in Colorectal Cancer. Int J Mol Sci. 2018 Sep 8;19(9). pii: E2672. doi: 10.3390/ijms19092672.

Huhn S, da Silva Filho MI, Sanmuganantham T, Pichulik T, Catalano C, Pardini B, Naccarati A, Polakova-Vymetálkova V, Jiraskova K, Vodickova L, Vodicka P, Löffler MW, Courth L, Wehkamp J, Din FVN, Timofeeva M, Farrington SM, Jansen L, Hemminki K, Chang-Claude J, Brenner H, Hoffmeister M, Dunlop MG, Weber ANR, Försti A. Coding variants in NOD-like receptors: An association study on risk and survival of colorectal cancer. PLoS One. 2018 Jun 21;13(6):e0199350. doi:10.1371/journal.pone.0199350.

Catalano C, da Silva Filho MI, Jiraskova K, Vymetalkova V, Levy M, Liska V, Vycital O, Naccarati A, Vodickova L, Hemminki K, Vodicka P, Weber ANR, Försti A. Short article: Influence of regulatory NLRC5 variants on colorectal cancer survival and 5-fluorouracil-based chemotherapy. Eur J Gastroenterol Hepatol. 2018 Aug;30(8):838-842. doi: 10.1097/MEG.0000000000001154.

IARC Monographs Vol 121 Group. Carcinogenicity of quinoline, styrene, and styrene-7,8-oxide. Lancet Oncol. 2018 Apr 18. pii: S1470-2045(18)30316-4. doi:10.1016/S1470-2045(18)30316-4.

Catalano, C., da Silva Filho, M. I., Frank, C., Jiraskova, K., Vymetalkova, V., Levy, M., Liska, V., Vycital, O., Naccarati, A., Vodickova, L., Hemminki, K., Vodicka, P.,Weber, A. N. R., Försti, A.: (2018). Investigation of single and synergic effects of NLRC5 and PD-L1 variants on the risk of colorectal cancer. PLoS ONE13(2).

Klein, A. P., Wolpin, B. M., Risch, H. A., Stolzenberg-Solomon, R. Z., Mocci, E., Zhang, M., Canzian, F., Childs, E.J., Hoskins, J.W., Jermusyk, A., Zhong, J., Chen, F., Albanes, D., Andreotti, G., Arslan, A.A., Babic, A., Bamlet, W.R., Beane-Freeman, L., Berndt, S.I., Blackford, A., Borges, M., Borgida, A., Bracci, P.M., Brais, L., Brennan, P., Brenner, H., Bueno-de-Mesquita, B., Buring, J., Campa, D., Capurso, G., Cavestro, G.M., Chaffee, K.G., Chung, C.C., Cleary, S., Cotterchio, M., Dijk, F., Duell, E.J., Foretova, L., Fuchs, C., Funel, N., Gallinger, S., M. Gaziano, J.M., Gazouli, M., Giles, G.G., Giovannucci, E., Goggins, M., Goodman, G.E., Goodman, P.J., Hackert, T., Haiman, C., Hartge, P., Hasan, M., Hegyi, P., Helzlsouer, K.J., Herman, J., Holcatova, I., Holly, E.A., Hoover, R., Hung, R.J., Jacobs, E.J., Jamroziak, K., Janout, V., Kaaks, R., Khaw, K.T., Klein, E.A., Kogevinas, M., Kooperberg, C., Kulke, M.H., Kupcinskas, J., Kurtz, R.J., Laheru, D., Landi, S., Lawlor, R.T., Lee, I.M., LeMarchand, L., Lu, L., Malats, N., Mambrini, A., Mannisto, S., Milne, R.L., Mohelníková-Duchoňová, B., Neale, R.E., Neoptolemos, J.P., Oberg, A.L., Olson, S.H., Orlow, I., Pasquali, C., Patel, A.V., Peters, U., Pezzilli, R., Porta, M., Real, F.X., Rothman, N., Scelo, G., Sesso, H.D., Severi, G., Shu, X.O., Silverman, D., Smith, J.P., Soucek, P., Sund, M., Talar-Wojnarowska, R., Tavano, F., Thornquist, M.D., Tobias, G.S., Van Den Eeden, S.K., Vashist, Y., Visvanathan, K., Vodicka, P., Wactawski-Wende, J., Wang, Z., Wentzensen, N., White, E., Yu, H., Yu, K., Zeleniuch-Jacquotte, A., Zheng, W., Kraft, P., Li, D., Chanock, S., Obazee, O., Petersen, G.M., Amundadottir, L. T. (2018). Genome-wide meta-analysis identifies five new susceptibility loci for pancreatic cancer. Nature Communications9(1), 556.

 Rejhova, A., Opattova, A., Cumova, A., Slíva, D., & Vodicka, P. (2018). Natural compounds and combination therapy in colorectal cancer treatment. European Journal of Medicinal Chemistry144, 582–594.

 

 

2017

Barontini, J., Antinucci, M., Tofanelli, S., Cammalleri, M., Dal Monte, M., Gemignani, F., Vodička, P., Marangoni, R., Vodičková, L., Kupcinskas, J., Vymetálková, V., Försti, A., Canzian, F., Stein, A., Moreno, V., Mastrodonato, N., Tavano, F., Panza, A., Barale, R., Landi, S., Campa, D.: (2017) Association between polymorphisms of TAS2R16 and susceptibility to colorectal cancer. BMC Gastroenterology. 17: 104.

Carrai, M., Campa, D., Vodička, P., Flamini, R., Martelli, I., Slyšková, J., Jirásková, K., Rejhová, A., Vodenková, S., Canzian, F., Bertelli, A., Dalla Vedova, A., Bavaresco, L., Vodičková, L., Barale, R.: (2017) Association between taste receptor (TAS) genes and the perception of wine characteristics. Scientific Reports. 7: 9239.

De Santi, Ch., Pucci, P., Bonotti, A., Melaiu, O., Cipollini, M., Silvestri, R., Vymetálková, V., Barone, E., Paolicchi, E., Corrado, A., Lepori, I., Dell'Anno, I., Pelle, L., Vodička, P., Mutti, L., Foddis, R., Cristaudo, A., Gemignani, F., Landi, S.: (2017) Mesothelin promoter variants are associated with increased soluble mesothelin-related peptide levels in asbestos-exposed individuals. Occupational and Environmental Medicine. 74 (6): 457-464.

Kroupa, M. , Polívková, Z., Rachakonda, S., Schneiderová, M., Vodenková, S., Buchler, T., Jirásková, K., Urbanová, M., Vodičková, L., Hemminki, K., Kumar, R., Vodička, P.: (2017) Bleomycin-induced chromosomal damage and shortening of telomeres in peripheral blood lymphocytes of incident cancer patients. Genes Chromosomes & Cancer. IN PRESS

Mohelníková-Duchoňová, B., Strouhal, O., Hughes, D. J., Holcatová, I., Oliverius, M., Kala, Z., Campa, D., Rizzato, C., Canzian, F., Pezzilli, R., Talar-Wojnarowska, R., Malecka-Panas, E., Sperti, C., Sperti, C., Federico Zambon, C., Pedrazzoli, S., Fogar, P., Milanetto, A.C., Capurso, G., Fave, G.D., Valente, R., Gazouli, M., Malleo, G., Lawlor, R.T., Strobel, O., Hackert, T., Giese, N., Vodička, P., Vodičková, L., Landi, S., Tavano, F., Gioffreda, D., Piepoli, A., Pazienza, V., Mambrini, A., Pedata, M., Cantore, M., Bambi, F., Ermini, S., Funel, N., Lemstrová, R., Souček, P.: (2017) SLC22A3 polymorphisms do not modify pancreatic cancer risk, but may influence overall patient survival. Scientific Reports. 7, 43812.

Schneiderová, B., Naccarati, A., Pardini, B., Rosa, F., Di Gaetano, C., Jirásková, K., Opattová, A., Levý, M., Veskrna, K., Veškrnová, V., Buchler, T., Landi, S., Vodička, P., Vymetálková, V.: (2017) MicroRNA-binding site polymorphisms in genes involved in colorectal cancer etiopathogenesis and their impact on disease prognosis. Mutagenesis. 32(5): 533-542.

Schormair, B., Zhao, Ch., Bell, S., Tilch, E., Salminen, A.V., Putz, B., Dauvilliers, Y., Stefani, A., Högl, B., Poewe, W., Kemlink, D., Šonka, K., Bachmann, C. G., Paulus, W., Trenkwalder, C., Oertel, W., Hornyak, M., Teder-Laving, M., Metspalu, A., Hadjigeorgiou, G.M., Polo, O., Fietze, I., Ross, O.A., Wszolek, Z., Butterworth, A.S., Soranzo, N., Ouwehand, W. H., Roberts, D.J., Danesh, J., Allen, R.P., Earley, Ch.J., Ondo, W.G., Xiong, L., Montplaisir, J., Gan-Or, Z., Perola, M., Vodička, P., Dina, Ch., Franke, A., Tittmann, L., Stewart, A.F.R., Shah, S.H., Gieger, Ch., Peters, A., Rouleau, G.A., Berger, K., Oexle, K., Di Angelantonio, E., Hinds, D.A., Müller-Myhsok, B., Winkelmann, J.: (2017) Identification of novel risk loci for restless legs syndrome in genome-wide association studies in individuals of European ancestry: a meta-analysis. Lancet Neurology. 16 (11): 898-907.

Schubert, S.A., Ruano, D., Elsayed, F.A., Boot, A., Crobach, S., Sarasqueta, A.F., Wolffenbuttel, B., van der Klauw, M.M., Oosting, J., Tops, C.M., van Eijk, R., Vasen, H.F., Vossen, R.H., Nielsen, M., Castellví-Bel, S., Ruiz-Ponte, C., Tomlinson, I., Dunlop, M.G., Vodička, P., Wijnen, J.T., Hes, F.J., Morreau, H., de Miranda, N.F., Sijmons, R.H., van Wezel, T.: (2017) Evidence for genetic association between chromosome 1q loci and predisposition to colorectal neoplasia. British Journal of Cancer. 117 (8): 1215-1223.

Thomsen, H., Campo, Ch., Weinhold, N., da Silva Filho, M.I., Pour, L., Gregora, E., Vodička, P., Vodičková, L., Hoffmann, P., Nöthen, M.M., Jöckel, K.H., Langer, Ch., Hájek, R., Goldschmidt, H., Hemminki, K., Försti, A.: (2017) Genomewide association study on monoclonal gammopathy of unknown significance (MGUS). European Journal of Haematology. 99 (1): 70-79.

Vymetálková, V. , Pardini, B., Rosa, F., Jirásková, K., Di Gaetano, C., Bendová, P., Levy, M., Veskrnová, V., Buchler, T., Vodičková, L., Naccarati, A., Vodička, P.: (2017) Polymorphisms in microRNA binding sites of mucin genes as predictors of clinical outcome in colorectal cancer patients. Carcinogenesis, 38(1): 28-39.

Wultsch, G., Nersesyan, A., Kundi, M., Mišík, M., Setayesh, T., Waldherr, M., Vodička, P., Vodičková, L., Knasmüller, S.: (2017) Genotoxic and Cytotoxic Effects in Exfoliated Buccal and Nasal Cells of Chromium and Cobalt Exposed Electroplaters. Journal of Toxicology and Environmental Health. Part A. 80(13-15): 651-660.

2016

Campa, D., Pastore, M., Gentiluomo, M., Talar-Wojnarowská, R., Kupcinskas, J., Malecka-Panas, E., Neoptolemos, J.P., Niesen, W., Vodička, P., Delle Fave, G., Bas Bueno-de-Mesquita, H., Gazouli M., Pacetti, P., Di Leo, M., Ito, H., Klüter, H., Souček, P., Corbo, V., Yamao, K., Hosono, S., Kaaks, R., Vashist, Y., Gioffreda, D., Strobel, O., Shimizu, Y., Dijk, F., Andriulli, A., Ivanauskas, A., Bugert, P., Tavano, F., Vodičková, L., Federico Zambon, C., Lovecek, M., Landi, S., Key, T.J., Boggi, U., Pezzilli, R., Jamroziak, K., Mohelnikova-Duchonová, B., Mambrini, A., Bambi, F., Busch, O., Pazienza, V., Valente, R., Theodoropoulos, G.E., Hackert, T., Capurso, G., Martina Cavestro, G., Pasquali, C., Basso, D., Sperti, C., Matsuo, K., Büchler, M., Khaw, K.T., Izbicki, J., Costello, E., Katzke, V., Michalski, C., Stepien, A., Rizzato, C., Canzian, F.: (2016) Functional single nucleotide polymorphisms within the cyclin-dependent kinase inhibitor 2A/2B region affect pancreatic cancer risk. Oncotarget, IN PRESS

Elsnerová, K., Mohelniková-Duchonová, B., Čeřovská, E., Ehrlichová, M., Gut, I., Rob, L., Skapa, P., Hruda, M., Bartaková, A., Bouda, J., Vodička, P., Souček, P., Václavíková, R.,: (2016) Gene expression of membrane transporters: Importance for prognosis and progression of ovarian carcinoma. Oncol. Rep., 35(4): 2159-2170.

Försti, A., Frank, C., Smolková, B., Kazimirová, A., Barancoková, M., Vymetálková, V., Kroupa, M., Naccarati, A., Vodičková, L., Buchancová, J., Dusinská, M., Musak, L., Vodička, P., Hemminki, K.: (2016) Genetic variation in the major mitotic checkpoint genes associated with chromosomal aberrations in healthy humans. Cancer Lett., 380(2):442-446.

Kořenková, V., Slyšková, J., Novosadová, V., Pizzamiglio, S., Langerová, L., Bjorkman, J., Vyčítal, O., Liska, V., Levy, M., Veskrna, K., Vodička, P., Vodičková, L., Kubista, M., Verderio, P.: (2016)The focus on sample quality: Influence of colon tissue collection on reliability of qPCR data. Sci Rep, 6: 29023.

Kunická, T., Procházka, P., Krus, I., Bendová, P., Protivová, M., Susová, S., Hlaváč, V., Liska, V., Novák, P., Schneiderová, M., Pitule, P., Bruha, J., Vyčítal, O., Vodička, P., Souček, P.: (2016) Molecular profile of 5-fluorouracil pathway genes in colorectal carcinoma. BMC Cancer, 16(1):795.

Naccarati, A. , Rosa, F., Vymetálková V., Barone, E., Jirásková, K., Di Gaetano, C., Novotný, J., Levy, M., Vodičková, L., Gemignani, F., Buchler, T., Landi, S., Vodička, P., Pardini, B.: (2016) Double-strand break repair and colorectal cancer: gene variants within 3' UTRs and microRNAs binding as modulators of cancer risk and clinical outcome. Oncotarget, 7(17): 23156-23169.

Vodička, P. , Musak, L., Fiorito, G., Vymetálková, V., Vodičková, L., Naccarati, A.: (2016) DNA and chromosomal damage in medical workers exposed to anaesthetic gases assessed by the lymphocyte cytokinesis-block micronucleus (CBMN) assay. A critical review. Mutat. Res.-Rev. Mutat. Res., 770(Pt A): 26-34.

Vodička, P., Pardini, B., Vymetálková, V., Naccarati, A.: (2016) Polymorphisms in Non-coding RNA Genes and Their Targets Sites as Risk Factors of Sporadic Colorectal Cancer. Adv.Exp.Med.Biol., 937: 123-149.

Vymetálková, V. , Pardini, B., Rosa, F., Jirásková, K., Di Gaetano, C., Bendová, P., Levy, M., Veskrnová, V., Buchler, T., Vodičková, L., Naccarati,A., Vodička, P.: (2016) Polymorphisms in microRNA binding sites of mucin genes as predictors of clinical outcome in colorectal cancer patients. Carcinogenesis, IN PRESS.

Vymetálková, V., Vodička, P. , Pardini, B., Rosa, F., Levy, M., Schneiderová, M., Liska, V., Vodičková, L., Nilsson, T.K., Farkas, S.A.: (2016) Epigenome-wide analysis of DNA methylation reveals a rectal cancer-specific epigenomic signature. Epigenomics, 8(9): 1193-1207.

Zhang, M., Wang, Z., Obazee, O., Jia, J., Childs, E.J., Hoskins, J., Figlioli, G., Mocci, E., Collins, I., Chung, C.C., Hautman, C., Arslan, A.A., Beane-Freeman, L., Bracci, P.M., Buring, J., Duell, E.J., Gallinger, S., Giles, G.G., Goodman, G.E., Goodman, P.J., Kamineni, A., Kolonel, L.N., Kulke, M.H., Malats, N., Olson SH, Sesso HD, Visvanathan K, White E, Zheng W, Abnet CC, Albanes D, Andreotti, G., Brais, L., Bueno-de-Mesquita, H.B., Basso, D., Berndt, S.I., Boutron-Ruault, M.C., Bijlsma, M.F., Brenner, H., Burdette, L., Campa, D., Caporaso, N.E., Capurso, G., Cavestro, G.M., Cotterchio, M., Costello, E., Elena, J., Boggi, U., Gaziano, J.M., Gazouli, M., Giovannucci, E.L., Goggins, M., Gross, M., Haiman, C.A., Hassan, M., Helzlsouer, K.J., Hu, N., Hunter, D.J., Iskierka-Jazdzewska, E., Jenab, M., Kaaks, R., Key, T.J., Khaw, K.T., Klein, E.A., Kogevinas, M., Krogh, V., Kupcinskas, J., Kurtz, R.C., Landi, M.T., Landi, S., Le Marchand, L., Mambrini, A., Mannisto, S., Milne, R.L., Neale, R.E., Oberg, A.L., Panico, S., Patel, A.V., Peeters, P.H., Peters, U., Pezzilli, R., Porta, M., Purdue, M., Quiros, J.R., Riboli, E., Rothman, N., Scarpa, A., Scelo, G., Shu, X.O., Silverman, D.T., Souček, P., Strobel, O., Sund, M., Małecka-Panas, E., Taylor, P.R., Tavano, F., Travis, R.C., Thornquist, M., Tjønneland, A., Tobias, G.S., Trichopoulos, D., Vashist, Y., Vodička, P., Wactawski-Wende, J., Wentzensen, N., Yu, H., Yu, K., Zeleniuch-Jacquotte, A., Kooperberg, C., Risch, H.A., Jacobs, E.J., Li, D., Fuchs, C., Hoover, R., Hartge, P., Chanock, S.J., Petersen, G.M., Stolzenberg-Solomon, R.S., Wolpin, B.M., Kraft, P., Klein, A.P., Canzian, F., Amundadottir, L.T.: (2016) Three new pancreatic cancer susceptibility signals identified on chromosomes 1q32.1, 5p15.33 and 8q24.21. Oncotarget, IN PRESS

2015

Aherne, S.T., Madden, S.F., Hughes, D.J., Pardini, B., Naccarati, A., Levy, M., Vodička, P., Neary, P., Dowling, P., Clynes, M.: (2015) Circulating miRNAs miR-34a and miR-150 associated with colorectal cancer progression. BMC Cancer. 15: 329.

Campa, D., Rizzato, C., Stolzenberg-Solomon, R., Pacetti, P., Vodička, P., Cleary, S.P., Capurso, G., Bueno-de-Mesquita, H.B., Werner, J., Gazouli, M., Butterbach, K., Ivanauskas, A., Giese, N., Petersen, G.M., Fogar, P., Wang, Z., Bassi, C., Ryska, M., Theodoropoulos, G.E., Kooperberg, C., Li, D., Greenhalf, W., Pasquali, C., Hackert, T., Fuchs, C.S., Mohelnikova-Duchonova, B., Sperti, C., Funel, N., Dieffenbach, A.K., Wareham, N.J., Buring, J., Holcátová, I., Costello, E., Zambon, C.F., Kupcinskas, J., Risch, H.A., Kraft, P., Bracci, P.M., Pezzilli, R., Olson, S.H., Sesso, H.D., Hartge, P., Strobel, O., Małecka-Panas, E., Visvanathan, K., Arslan, A.A., Pedrazzoli, S., Souček, P., Gioffreda, D., Key, T.J., Talar-Wojnarowská, R., Scarpa, A., Mambrini, A., Jacobs, E.J., Jamroziak, K., Klein, A., Tavano, F., Bambi, F., Landi, S., Austin, M.A., Vodičková, L., Brenner, H., Chanock, S.J., Delle Fave, G., Piepoli, A., Cantore, M., Zheng, W., Wolpin, B.M., Amundadottir, L.T., Canzian, F.: (2015) TERT gene harbors multiple variants associated with pancreatic cancer susceptibility. Int J Cancer. 1;137(9):2175-2183.

Childs, E.J., Mocci, E., Campa, D., Bracci, P.M., Gallinger, S., Goggins, M., Li, D., Neale, R.E., Olson, S.H., Scelo, G., Amundadottir, L.T., Bamlet, W.R., Bijlsma, M.F., Blackford, A., Borges, M., Brennan, P., Brenner, H., Bueno-de-Mesquita, H.B., Canzian, F., Capurso, G., Cavestro, G.M., Chaffee, K.G., Chanock, S.J., Cleary, S.P., Cotterchio, M., Foretová, L., Fuchs, C., Funel, N., Gazouli, M., Hassan, M., Herman, J.M., Holcatová, I., Holly, E.A., Hoover, R.N., Hung, R.J., Janout, V., Key, T.J., Kupcinskas, J., Kurtz, R.C., Landi, S., Lu, L., Malecka-Panas, E., Mambrini, A., Mohelniková-Duchonová, B., Neoptolemos, J.P., Oberg, A.L., Orlow, I., Pasquali, C., Pezzilli, R., Rizzato, C., Saldia, A., Scarpa, A., Stolzenberg-Solomon, R.Z., Strobel, O., Tavano, F., Vashist, Y.K., Vodička, P., Wolpin, B.M., Yu, H., Petersen, G.M., Risch, H.A., Klein, A.P.: (2015) Common variation at 2p13.3, 3q29, 7p13 and 17q25.1 associated with susceptibility to pancreatic cancer. Nat Genet. 47(8):911-916.

Dowling, P., Hughes, D. J., Larkin, A. M., Meiller, J., Henry, M., Meleady, P., Lynch, V., Pardini, B., Naccarati, A., Levy, M., Vodička, P., Neary, P., Clynes, M.: (2015) Elevated levels of 14-3-3 proteins, serotonin, gamma enolase and pyruvate kinase identified in clinical samples from patients diagnosed with colorectal cancer. Clin. Chim. Acta. 441: 133-141.

Hemminki, K., Frank, C., Försti, A., Musak, L., Kazimirova, A., Barancokova, M., Horska, A., Vymetalková, V., Šmerhovský, Z.,Naccarati, A., Souček, P., Vodičková, L., Buchancová, J., Smolková, B., Dušinská, M., Vodička, P.: (2015) Metabolic gene variants associated with chromosomal aberrations in healthy humans. Gene Chromosomes Cancer. 54(4): 260-266.

Hemminki, K., Rachakonda, S., Musak, L., Vymetálková, V., Halasová, E., Försti, A., Vodičková, L., Buchancová, J., Vodička, P., Kumar, R.: (2015) Telomere length in circulating lymphocytes: Association with chromosomal aberrations. Gene Chromosomes Cancer. 54(3): 194-196.

Pardini, B., Rosa, F., Naccarati, A., Vymetálková, V., Ye, Y., Wu, X., di Gaetano, C., Buchler, T., Novotný, J., Matullo, G., Vodička, P.: (2015) Polymorphisms in microRNA genes as predictors of clinical outcomes in colorectal cancer patients. Carcinogenesis. 31(6): 82-86.

Slyšková, J., Cordero, F., Pardini, B., Korenková, V., Vymetalková, V., Bielik, L., Vodičková, L., Pitule, P., Liska, V., Matejka, V. M., Levy, M., Buchler, T., Kubista, M., Naccarati, A., Vodička, P.: (2015) Post-treatment recovery of suboptimal DNA repair capacity and gene expression levels in colorectal cancer patients. Mol. Carcinog., 54 (9): 769-778.

Vodenková, S., Polivková, Z., Musak, L., Šmerhovský, Z., Zoubková, H., Sytarová, S., Kavcová, E., Halasová, E., Vodičková, L., Jirásková, K., Svoboda, M., Ambrus, M., Hemminki, K., Vodička, P.: (2015) Structural chromosomal aberrations as potential risk markers in incident cancer patients. Mutagenesis.30(4):557-563.

Vodička, P., Caja, F., Vymetálková, V., Procházka, P., Vodičková, L., Schwarzová, L., Slyšková, J., Kumar, R., Schneiderová, M.: (2015) Ile68Met germline variant in exon 2 of the mutL homolog 1 gene in a colorectal cancer patient. Oncol. Lett. 9(1): 183-186.

Vodička, P., Musak, L., Frank, C., Kazimirova, A., Vymetálková, V., Barancoková, M., Smolková, B., Dzupinková, Z., Jirasková, K., Vodenková, S., Kroupa, M., Osina, O., Naccarati, A., Palitti, F., Försti, A., Dusinská, M., Vodičková, L., Hemminki, K.: (2015) Interactions of DNA repair gene variants modulate chromosomal aberrations in healthy subjects. Carcinogenesis.36(11): 1299-1306.

Vymetálková, V., Souček, P., Kunicka, T., Jirásková, K., Brynychova, V., Pardini, B., Novosadova, V., Polivkova, Z., Kubackova, K., Kozevnikovova, R., Ambrus, M., Vodičková, L., Naccarati, A., Vodička, P.: (2015) Genotype and Haplotype Analyses of TP53 Gene in Breast Cancer Patients: Association with Risk and Clinical Outcomes. PLoS One. 10(7): e0134463.

2014

Farkas, S. A., Vymetálková, V., Vodičková, L., Vodička, P., Nilsson, T. K.: (2014) DNA methylation changes in genes frequently mutated in sporadic colorectal cancer and in the DNA repair and Wnt/β-catenin signaling pathway genes. Epigenomics 6(2): 179-191.

Huhn, S., Bevier, M., Pardini, B., Naccarati, A., Vodičková, L., Novotny, J., Vodička, P., Hemminki, K., Försti, A.: (2014) Colorectal cancer risk and patients' survival: influence of polymorphisms in genes somatically mutated in colorectal tumors. Cancer Causes Control. 25(6): 759-769.

Kunická, T., Václavíková, R., Hlaváč, V., Vrána, D., Pecha, V., Rauš, K., Trnková, M., Kubáčková, K., Ambruš, M., Vodičková, L., Vodička, P., Souček, P.: (2014) Non-Coding Polymorphisms in Nucleotide Binding Domain 1 in ABCC1 Gene Associate with Transcript Level and Survival of Patients with Breast Cancer. PLoS One 9(7): e101740.

Lu, S., Pardini, B., Cheng, B., Naccarati, A., Huhn, S., Vymetálková, V., Vodičková, L., Buchler, T., Hemminki, K., Vodička, P., Försti, A.: (2014) Single Nucleotide Polymorphisms within Interferon Signaling Pathway Genes Are Associated with Colorectal Cancer Susceptibility and Survival. PLoS One 9(10): e111061.

Makovický, P., Tumova, E., Volek, Z., Makovický, P., Vodičková, L., Slyšková, J., Svoboda, M., Rejhová, A., Vodička, P., Samasca, G., Králová, A., Nagy, M., Mydlarova-Blascaková, M., Poracova, J.: (2014) Histopathological aspects of liver under variable food restriction: Has the intense one-week food restriction a protective effect on non-alcoholic-fatty-liver-disease (NAFLD) development?Pathol. Res. Pract. 210(12): 855-862.

Makovický, P., Tůmová, E., Volek, Z., Makovický, P., Vodička, P.: (2014) Histological aspects of the small intestine under variable feed restriction: The effects of short and intense restriction on a growing rabbit model. Exp. Ther. Med. 8(5): 1623-1627.

Pardini, B., Verderio, P., Pizzamiglio, S., Nici, C., Maiorana, M. V., Naccarati, A., Vodičková, L., Vymetálková, V., Veneroni, S., Daidone, M. G., Ravagnani, F., Bianchi, T., Bujenda, L., Carracedo, A., Castells, A., Ruiz-Ponte, C., Morreau, H., Howarth, K., Jones, A., Castellvı´-Bel, S., Li L., Tomlinson, I., Van Wezel, T., Vodička, P., Radice, P., Peterlongo, P., the EPICOLON Consortium: (2014) Association between CASP8 –652 6N Del Polymorphism (rs3834129) and Colorectal Cancer Risk: Results from a Multi-Centric Study. PLoS ONE 9(3): e91310.

Pardini, B., Bermejo, J. L., Naccarati, A., Di Gaetano, C., Rosa, F., Legrand, C., Novotny, J., Vodička, P., Kumar, R.: (2014) Inherited variability in a master regulator polymorphism (rs4846126) associates with survival in 5-FU treated colorectal cancer patients.Mutat. Res.-Fundam. Mol. Mech. Mutagen. 766-767: 7-13.

Svoboda, M., Slyšková, J., Schneiderova, M., Makovicky, P., Bielik, L., Levy, M., Lipska, L., Hemmelova, B., Kala, Z., Protivankova, M., Vycital, O., Liska, V., Schwarzova, L., Vodičková, L, Vodička P.: (2014) HOTAIR long non-coding RNA is a negative prognostic factor not only in primary tumors, but also in the blood of colorectal cancer patients. Carcinogenesis. 35(7): 1510-1515.

Slyšková, J., Lorenzo, Y., Karlsen, A., Carlsen, M. H., Novosadová, V., Blomhoff, R., Vodička, P., Collins, A. R.: (2014) Both genetic and dietary factors underlie individual differences in DNA damage levels and DNA repair capacity. DNA Repair (Amst). 16 :66-73.

Slyšková, J., Langie, S, A. , Collins, A. R., Vodička, P.: (2014) Functional evaluation of DNA repair in human biopsies and their relation to other cellular biomarkers. Front Genet. 5: 116.

Švec, J., Schwarzová, L., Janošíková, B., Štekrová, J., Mandys, V., Kment, M., Vodička, P.: (2014) Synchronous gastric and sebaceous cancers, a rare manifestation of MLH1-related Muir-Torre syndrome. Int. J. Clin. Exp. Pathol. 7(8): 5196-5202.

Vymetálková, V., Pardini, B., Rosa, F., Di Gaetano, C., Novotny, J., Levy, M., Buchler, T., Slyšková, J., Vodičková, L., Naccarati, A., Vodička, P.: (2014) Variations in mismatch repair genes and colorectal cancer risk and clinical outcome. Mutagenesis. 29(4): 259-265.

Vymetálková, V., Slyšková, J., Korenková, V., Bielik, L., Langerová, L., Procházka, P., Rejhová, A., Schwarzová, L., Pardini, B.,Naccarati, A., Vodička, P.: (2014) Molecular characteristics of mismatch repair genes in sporadic colorectal tumors in Czech patients BMC Med. Genet. 15(1) :17.

Wolpin, B. M., Rizzato, C., Kraft, P., Kooperberg, C., Petersen, G. M., Wang, Z., Arslan, A. A., Beane-Freeman, L., Bracci, P.M., Buring, J., Canzian, F., Duell, E. J., Gallinger, S., Giles, G. G., Goodman, G. E., Goodman, P. J., Jacobs, E. J., Kamineni, A., Klein, A. P., Kolonel, L. N., Kulke, M. H., Li, D., Malats, N., Olson, S. H., Risch, H. A., Sesso, H. D., Visvanathan, K., White, E., Zheng, W., Abnet, C. C., Albanes, D., Andreotti, G., Austin, M. A., Barfield, R., Basso, D., Berndt, S. I., Boutron-Ruault, M. C., Brotzman, M., Büchler, M. W., Bueno-de-Mesquita, H. B., Bugert, P., Burdette, L., Campa, D., Caporaso, N. E., Capurso, G., Chung, C., Cotterchio, M., Costello, E., Elena, J., Funel, N., Gaziano, J. M., Giese, N. A., Giovannucci, E. L., Goggins, M., Gorman, M. J., Gross, M., Haiman, C. A., Hassan, M., Helzlsouer, K. J., Henderson, B. E., Holly, E. A., Hu, N., Hunter, D. J., Innocenti, F., Jenab, M., Kaaks, R., Key, T. J., Khaw, K. T., Klein, E. A., Kogevinas, M., Krogh, V., Kupcinskas, J., Kurtz, R. C., LaCroix, A., Landi, M. T., Landi, S., Le Marchand, L., Mambrini, A., Mannisto, S., Milne, R. L., Nakamura, Y., Oberg, A. L., Owzar, K., Patel, A. V., Peeters, P. H., Peters, U., Pezzilli, R., Piepoli, A., Porta, M., Real, F. X., Riboli, E., Rothman, N., Scarpa, A., Shu, X. O., Silverman, D. T., Soucek, P., Sund, M., Talar-Wojnarowska, R., Taylor, P. R., Theodoropoulos, G. E., Thornquist, M., Tjønneland, A., Tobias, G. S., Trichopoulos, D., Vodička, P., Wactawski-Wende, J., Wentzensen, N., Wu, C., Yu, H., Yu, K., Zeleniuch-Jacquotte, A., Hoover, R., Hartge, P., Fuchs, C., Chanock, S. J., Stolzenberg-Solomon, R. S., Amundadottir, L. T.: (2014) Genome-wide association study identifies multiple susceptibility loci for pancreatic cancer. Nature Genet. 46(9): 994-1000.

2013

Azqueta, A., Langie, S. A., Slyšková, J., Collins, A. R.: (2013) Measurement of DNA base and nucleotide excision repair activities in mammalian cells and tissues using the comet assay - A methodological overview. DNA Repair (Amst) 12: 1007-1010.

Campa, D., Rizzato, C., Capurso, G., Giese, N., Funel, N., Greenhalf, W., Souček, P., Gazouli, M., Pezzilli, R, Pasquali, C., Talar-Wojnarowska, R., Cantore, M., Andriulli, A., Scarpa, A., Jamroziak, K., Delle Fave, G., Costello, E., Khaw, K. T., Heller, A., Key, T. J., Theodoropoulos, G., Malecka-Panas, E., Mambrini, A., Bambi, F., Land, S., Pedrazzoli, S., Bassi, C., Pacetti, P., Piepoli, A., Tavano, F., di Sebastiano, P., Vodičková, L., Basso, D., Plebani, M., Fogar, P., Büchler, M. W., Bugert, P., Vodička, P., Boggi, U., Neoptolemos, J. P., Werner, J., Canzian, F.: (2013) Genetic susceptibility to pancreatic cancer and its functional characterisation: The PANcreatic Disease ReseArch (PANDoRA) consortium. Dig. Liver Dis. 45(2): 95-99.

Campa, D., Rizzato, C., Bauer, A. S., Werner, J., Capurso, G., Costello, E., Talar-Wojnarowska, R., Jamroziak, K., Pezzilli, R., Gazouli, M., Khaw, K. T., Key, T. J., Bambi, F., Mohelnikova-Duchonova, B., Heller, A., Landi, S., Vodičková, L., Theodoropoulos, G., Bugert, P.,Vodička, P., Hoheisel, J., Delle Fave, G., Neoptolemos, J., Souček, P., Buchler, M. W., Giese, N. A., Canzian F.: (2013) Lack of replication of seven pancreatic cancer susceptibility lociidentified in two Asian populations. Cancer Epidemiol. Biomarkers Prev.22(2): 320-323.

Lu, S., Bevier, M., Huhn, S., Sainz, J., Lascorz, J., Pardini, B., Naccarati, A., Vodičková, L., Novotný, J., Hemminki, K., Vodička, P., Försti A.: (2013) Genetic variants in C-type lectin genes are associated with colorectal cancer susceptibility and clinical outcome.Int. J. Cancer. 133(10): 2325-2333.

Makovicky, P., Rimarova, K., Boor, A., Makovicky, P., Vodička, P., Samasca, G., Kruzliak, P.: (2013) Correlation between antibodies and histology in celiac disease: Incidence of celiac disease is higher than expected in the pediatric population. Mol. Med. Rep.8(4): 1079-1083.

Mušák, L., Šmerhovský, Z., Halasová, E., Osina, O., Letková, L., Vodičková, L., Poláková, V., Buchancová, J., Hemminki, K.,Vodička, P.: (2013) Chromosomal damage among medical staff occupationally exposed to volatile anesthetics, antineoplastic drugs, and formaldehyde. Scand. J. Work Environ. Health. 39(6): 618-630.

Pardini, B., Rosa, F., Barone, E., Di Gaetano, C., Slyšková, J., Novotný, J., Levy, M., Garritano, S., Vodičková, L., Buchler, T., Gemignani, F., Landi, S., Vodička, P., Naccarati, A.: (2013) Variation within 3' UTRs of base excision repair genes and response to therapy in colorectal cancer patients: a potential modulation of microRNAs binding. Clin. Cancer Res. 19(21): 6044-6056.

Picelli, S., Bermejo, J. L., Change-Claude, J., Hoffmeister, M., Fernández-Rozadilla, C., Carracedo, A., Castells, A., Castellví-Bel, S.,Naccarati, A., Pardini, B., Vodičková, L., Hüller, H., Talseth-Palmer, B. A., Stibbard, G., Peterlongo, P., Nici, C., Veneroni, S., Li, L., Casey, G., Tenesa, A., Farrington, S. M., Tomlinson, I., Moreno, V., van Wezel, T., Wijnen, J., Dunlop, M., Radice, P., Scott, R. J.,Vodička, P., Ruiz-Ponte, C., Brenner, H., Buch, S., Völzke, H., Hampe, J., Schafmayer, C., Lindblom, A.: Meta-Analysis of Mismatch Repair Polymorphisms within the Cogent Consortium for Colorectal Cancer Susceptibility; PLoS One 8(9): e72091.

Poláková-Vymetálková, V., Vannucci, L., Korenková, V., Procházka, P., Slyšková, J., Vodičková, L., Rusňáková, V., Bielik, L., Burocziová, M., Rossmann., P., Vodička, P.: (2013) Evaluation of tumor suppressor gene expressions and aberrant methylation in the colon of cancer-induced rats: a pilot study. Mol. Biol. Rep. 40(10): 5921-5929.

Procházka, P., Hrabeta, J., Vicha, A., Cipro, S., Stejskalová, E., Musil, Z., Vodička, P., Eckschlager, T.: (2013) Changes in MYCN expression in human neuroblastoma cell lines following cisplatin treatment may not be related to MYCN copy numbers. Oncol. Rep. 29(6):2415-2421.

Rizzato, C., Campa, D., Pezzilli, R., Souček, P., Greenhalf, W., Capurso, G., Talar-Wojnarowska, R., Heller, A., Jamroziak, K., Khaw, K. T., Key, T. J., Bambi, F., Landi, S., Mohelniková-Duchoňová, B., Vodičková, L., Büchler, M. W., Bugert. P., Vodička, P., Neoptolemos, J. P., Werner, J., Hoheisel, J. D., Bauer, A. S., Giese, N., Canzian, F.: (2013) ABO blood groups and pancreatic cancer risk and survival: Results from the PANcreatic Disease ReseArch (PANDoRA) consortium. Oncol. Rep. 29(4): 1637-1644.

AZV 15-27580A Taste perception, oxidative damage and colon microenvironment in colorectal carcinogenesis: impacts on the disease risk, prognosis and prevention. PI: Dr. Vodička, (2015-2019).

AZV 15-26535A The role of genetic variations in microRNA genes and in microRNA binding sites in colorectal cancer in relation to personalized therapy. PI: Ing. Vymetálková, (2015-2019).

AZV 17-30920A Characterization of acquired chemoresistance by DNA repair gene profiling in colon cancer patients through non-invasive repeated analysis. PI: Ing. Vymetálková, (2017-2019).

GACR 17 – 16857S Identification and comparison of plasma/stool miRNA signature in colorectal cancer by next generation sequencing. PI: Dr. Naccarati, (2017 – 2019).

GACR 18- 09709S  Mapping the traits involved in malignant transformation of colorectal adenoma into carcinoma. PI: Dr. Vodička, (2018-2020).

NV 18-03-00199 Identification of diagnostic markers in colorectal cancer by profiling of trascriptome, DNA methylome and microbiome in adenoma-carcinoma transition. PI: Dr. Vodička, (2018-2022).

Thomayer hospital Prague

IKEM, Prague

General teaching hospital, Prague

Teaching hospital Pilsen, Pilsen

Teaching hospital Kralovske Vinohrady, Prague

Masaryk University, Brno

First Medical Faculty, Charles University, Prague

Third Medical Faculty, Charles University, Prague

Biomedical Centre, Faculty of Medicine, Pilsen, Charles University, Prague

National Institute of Public Health, Prague

Institute of Biotechnology, CAS v.v.i., Vestec

Institute of Molecular Genetics, ASCR, Prague

Institute of Microbiology, ASCR, Prague

CEITEC - central european institute of technology, Brno

DKFZ (German Cancer Research Center) Heidelberg, Germany (prof. Hemminki, prof. Kumar, Dr. Canzian, Dr. Főrsti)

University of Pisa, Italy (prof. Landi, prof. Barale, dr. Campa)

Italian Institute for Genomic Medicine, Torino, Italy (Dr. Naccarati, Dr. Pardini)

Leiden University Medical Center, Leiden, Holland (prof. Van Wezel)

IDIBAPS / CIBERehd / Hospital Clínic, Barcelona, Spain (Dr. Castellvi-Bel)

Umeå University, Sweden (Prof. Torbjőrn Nilsson)

Vienna Medical University, Austria (prof. Knasműller, Dr.Gsur)

RCSI Royal College of Surgeons in Ireland, Ireland (Dr. Hughes)

Genetics and Epidemiology of Colorectal cancer Consortium (GECCO)

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Contacts

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