Institute of Experimental Medicine CAS

The research of the Department is focused on the development of artificial tissues, mainly biodegradable scaffolds for tissue regeneration, such as nanofibers, foams, and hydrogels for the regeneration of cartilage, bone and incisional hernia. We also focus on computer modeling of protein structures. We are developing the technology of controlled drug delivery from nanofibers scaffolds with liposomes for targeted release of drugs into the defect. The work is also concentrated on the development of three-dimensional nanofibers, using novel technique of Forcespinning®. These nanofibres are more suitable for cell growth and differentiation. Moreover, high on our priority list is also the accelerated transfer of newly developed technologies and know-how into clinical practice. We are developing artificial scaffolds for the regeneration of bone and cartilage in clinical practice.

Deputy Head:

Michala Rampicová, MSc, PhD.
E-mail: m.rampichova@biomed.cas.cz
Phone: +420 241 062 387

Research Scientists:

Eva Filová, MSc, PhD.
Andrej Litvinec, MSc, PhD.
Michala Rampichová, MSc, PhD. 
Andrea Staffa, MSc, PhD. (ML)
Jana Daňková, MSc, PhD. (ML)
Giuseppe Bitti, MSc., PhD.
Gracián Tejral, MSc, PhD.
Bruno Sopko, PhD.

PhD. Students:

Martin Královič, MSc
Karolína Vocetková, MD et Dipl. Ing.
Věra Sovková, MSc
Radek Divín, MSc
Věra Lukášová, MSc
Barbora Voltrová, MSc
Veronika Blahnová, CSc.

Undergraduate Students:

Gabriela Korbelová
Erna Šljivnjak

Technican:

Helena Smolová

Important results in 2017

1.Self-assembling nanoparticles encapsulating zoledronic acid inhibit mesenchymal stromal cells (MSCs) differentiation, migration and secretion of proangiogenic factors and their interactions with prostate cancer cells

We developed self-assembling nanoparticles encapsulating zoledronic acid (NZ) that allowed a higher intratumor delivery of the drug compared with free zoledronic acid in in vivo cancer models of prostate cancer ( PCa). The treatment with NZ decreased migration and differentiation into adipocytes and osteoblasts of MSCs and inhibited secretion of proangiogenic factors.  In conclusion, NZ was capable to inhibit the cross talk between MSCs and PCa which explain the anticancer activity of NZ on PCa.

 

filova-2017-1

Treatment of MSCs with nanoparticles encapsulating zoledronic acid (NZ)  or treatment with zoledronic acid solution (ZA) decreased the clonogenic growth of prostate cancer cells 3 (PC3) induced by conditioned medium of MSCs (MSC-CM). 100 PC3 cells were plated in 24-well flat-bottomed plates and allowed to adhere for 24 h, then cultured in the presence of increased concentrations of supernatants from MSCs untreated or treated with ZA or NZ (20 μM). After 7 days, plates were observed under phase-contrast microscopy and colonies counted. Values represent the mean ± SD of N=3 independent experiments.

Publication : Borghese, C., Casagrande, N.,  Pivetta, E., Colombatti, A., Boccellino, M., Amler, E., Normanno, N., Caraglia, M., De Rosa, G., Aldinucci, D.: (2017) C OncoTarget. 8 (26): 42926-42938.

2.Platelet-functionalized three-dimensional poly-Ɛ-epsilon-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors.

PCL three-dimensional fibrous meshes prepared via centrifugal spinning were combined with adhered platelets of five different concentrations. Released growth factors supported cell proliferation and metabolic activity of MG-63 cells in concentrations higher than physiological (300×109/L). Lower concentrations of platelets were not supportive and were comparable to control. Similarly, alkaline phosphatase activity was increased on samples with two most concentrated platelets concentrations.

filova-2017-2

Platelets adhered on poly- ε-caprolactone fibers. SEM visualization of platelet adhesion on PCL fibers. Platelets were partially activated and formed a fibrin net 24 h after adhesion (A). Platelets were visibly adhered on fibers even after 14 days of the experiment (B).

Publication: Rampichová, M., Buzgo, M., Míčková, A., Vocetková, K., Sovková, V., Lukášová, V., Filová, E., Rustichelli, F., Amler, E.: (2017) Platelet-functionalized three-dimensional polye-epsilon-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors. International Journal of Nanomedicine. 12:347-361.

3. Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides

The present study focuses on comparison of bioactive peptides as osteogenesis promoting factor. The chosen peptides were derived from receptor binding sequences of collagen III, BMP-7 and BMP-2. BMP-2 peptide has the best potential to induce osteogenic differentiation of pMSCs.

filova-2017-3

Expression of osteocalcin gene. The expression level of osteocalcin, which is a late marker of osteogenic differentiation, was detected on days 7 and 14. The abbreviations above the bars denote to statistical difference with P < 0.05. Abbreviations: MSCs, mesenchymal stem cells; OCN, osteocalcin; aminoacid sequences: I, IAGVGGEKSGGF; G, GQGFSYPYKAVFSTQ; K, KIPKASSVPTELSAISTLYL; 1, 1 μg/mL concentration of peptides; 5, μg/mL; 10, μg/mL; Cn, control group (with no peptides added).

Publication: Lukasova V., Buzgo M., Sovkova V., Dankova J., Rampichova M., Amler E., Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides. Cell Prolif, 2017. 50(4): p. e12357-n/a.

Important results in 2015

1. We have developed dispersion nanofibers from poly-ε-caprolactone enriched with magnetic nanoparticles prepared by needleless electrospinning

The nanofibers enhanced adhesion and osteogenic proliferation of pig mesenchymal stem cells and are promising for bone regeneration. (Daňková et al. 2015).

amler2015-1

Scanning electron microscopy of the poly-ε-caprolactone nanofiber scaffold with magnetic nanoparticles.

2. We have developed polypropylene (PP) surgical mesh coated with PCL nanofibers with adhered thrombocytes as natural source of growth factors

The composite mesh with thrombocytes showed improved fibroblasts adhesion, proliferation, and metabolic activity compared to PP, PP coated with nanofibers, and PP functionalized with thrombocytes. The system of composite scaffold with growth factors released from thrombocytes is promising approache for tissue engineering.

amler2015-2

Scanning electron microscopy of the implanted scaffolds. (A) PCL nanofibers; (B) PP mesh; (C) PP mesh functionalized with PCL nanofibers.

3. Nanofibers from polyvinyl alcohol (PVA) were functionalized by polyethylene glycol with biotin (PEG-b) linker and sequence-specific binding of avidin- antibody conjugate

PEG-b functionalized nanofibers significantly decreased nanofiber decay in a controlled manner. Moreover, the binding of anti CD-29 antibody to PEG-b linker stimulated mesenchymal stem cell adhesion to PVA-PEG-b nanofibers through β1-integrin receptor. The second system of the selective protein binding on the nanofiber surface represented anti-transferrin-PEG-b nanofibers.

amler2015-3

Photomicrograph and schema of nanofibers from polyvinylalcohol (PVA) functionalized with polyethylene glycol with biotin (PEG-b) linker and sequence-specific binding of avidin- antibody (anti-transferin) conjugate.

Publications:

  • Jana Daňková, Matej Buzgo,Jana Vejpravová, Simona Kubíčková,Věra Sovková, Lucie Vysloužilová, Alice Mantlíková, Alois Nečas, Evžen Amler. Highly efficient mesenchymal stem cell proliferation onPCL nanofibers with embedded magnetic nanoparticles. Int J Nanomedicine. 2015 Dec 7;10:7307-17. IF 4.19
  • Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E. 2015. Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution. Int J Nanomedicine. 2015 Apr 1;10:2635-2646. IF 4.19
  • Buzgo M, Greplová J, Soural M , Dagmar Bezděková, Andrea Míčková, Olga Kofroňová, Oldřich Benada, Jan Hlaváč, Evžen Amler. PVA immunonanofibers with controlled decay. Polymer 2015 Oct 23; 77:387-398 IF = 3.562

 Important results in 2014

1. Functionalized nanofibers for controlled drug delivery

The system of functionalized nanofibers with controlled drug delivery has been developed and optimized. This system has been applied for treatment of incisional hernia. Polypropylene surgical mesh was modified by PCL nanofibers covering and functionalised with adhesion of growth factors. Samples were tested in vivo on a rabbit model as a model for prevention of incisional hernia formation.

amler2014-1

Scanning electron microscopy of the scaffolds used for the abdominal closure. Notes: (A) nanofibers from poly-ε-caprolactone (magnification 230×); (B) polypropylene mesh (magnification 18×); (C) polypropylene mesh functionalized with poly-ε-caprolactone nanofibers (magnification 18×).

Collaboration:  Institute of Biomedical Engineering, Czech Technical University in Prague, Kladno; University Centre for Energy Efficient Buildings

Publications:

  • Amler E, Filová E, Buzgo M, Prosecká E, Rampichová M, Nečas A, Nooeaid P, Boccaccini AR, (2014): Functionalized nanofibers as drug-delivery systems for osteochondral regeneration. Nanomedicine-UK 9(7): 1083–1094, IF 5.9
  • Plencner, M., et al.: Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-ε-caprolactone nanofibers and growth factors for prevention of incisional hernia formation. Int. J. Nanomed. 9: 3263-3277, IF 4.2 
2. Biomechanical testing of the repaired abdominal wall

Abdominal closure was reinforced by application of polypropylene mesh functionalized with poly-ε-caprolactone nanofibers and growth factors. This novel arrangement is going to be used for prevention of incisional hernia formation. However, the system seems to be very general and there is intended for much broader chirurgical and orthopedical application.

amler2014-2

Images of carriers used for closure of abdominal incision switched by scanning electron microscopy. (A) nanofibers of poly-ε-caprolactone (magnification × 230), (B) polypropylene mesh (18 × magnification), (C) polypropylene mesh using functionalized nanofibers of poly-ε-caprolactone.

amler2014-3

Histological evaluation. Collagen, adipose tissue, and granulomatous infiltration in the scaffolds under study. In samples without any mesh (A), the incision was healing with a mixture of collagen (black arrow), adipose connective tissue (red arrow) and inflammatory infiltrate (yellow arrow). Samples with polypropylene (PP) mesh (B) had a high fraction of adipose tissue, but the spaces showing the dissolved mesh (black arrows) were surrounded by only a few inflammatory cells. Remnants of the nanofibers (C, D, E, F) were surrounded by granulomatous leukocyte-rich connective tissue (yellow arrows in C, D, E, F). The highest fraction of collagen (red arrow) was in samples of PCL nanofibers with adhered growth factors (GF) (D), followed by samples with no mesh (A) and by samples of PCL nanofibers (F). Low fractions of adipose tissue were found in samples of PCL nanofibers with adhered GF (D), samples with no mesh (A) and in samples of PCL nanofibers (F).

Publication: Plencner M, East B, Tonar Z, Otáhal M, Prosecká E, Rampichová M, Krejčí T, Litvinec A, Buzgo M, Míčková A, Nečas A, Hoch J, Amler E, (2014): Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-ε-caprolactone nanofibers and growth factors for prevention of incisional hernia formation. Int. J. Nanomed. 9: 3263-3277, IF 4.195

2017

Borghese, C., Casagrande, N., Pivetta, E., Colombatti, A., Boccellino, M., Amler, E., Normanno, N., Caraglia, M., De Rosa, G., Aldinucci, D.: (2017) Self-assembling nanoparticles encapsulating zoledronic acid inhibit mesenchymal stromal cells differentiation, migration and secretion of proangiogenic factors and their interactions with prostate cancer cells. OncoTarget. 8 (26): 42926-42938.

Buzgo, M., Rampichová, M., Vocetková, K., Sovková, V., Lukášová, V., Doupnik, M., Míčková, A., Rustichelli, F., Amler, E.: (2017) Emulsion centrifugal spinning for production of 3D drug releasing nanofibres with core/shell structure. RSC Advances. 7(3): 1215-1228.

Buzgo, M., Filová, E., Staffa, A., Rampichová, M. , Doupnik, M., Vocetková, K., Lukášová, V., Kolcun, R., Lukáš, D., Nečas, A., Amler, E.: (2017) Needleless emulsion electrospinning for the regulated delivery of susceptible proteins. Journal of Tissue Engineering and Regenerative Medicine. May 16. doi: 10.1002/term.2474. [Epub ahead of print]

Gregor, A., Filová, E., Novák, M., Kronek, J., Chlup, H., Buzgo, M., Blahnová, V., Lukášová, V., Bartoš, M., Nečas, A., Hošek, J.: (2017) Designing of PLA scaffolds for bone tissue replacement fabricated by ordinary commercial 3D printer. Journal of Biological Engineering. 11: 31.

Hadraba, D., Janáček, J., Filová, E., Lopot, F., Paesen, R., Fanta, O., Jarman, A., Nečas, A., Ameloot, M., Jelen, K.: (2017) Calcaneal Tendon Collagen Fiber Morphometry and Aging. Microscopy and Microanalysis. 23 (5): 1040-1047.

Lukášová, V., Buzgo, M., Sovková, V., Daňková, J., Rampichová, M., Amler, E.: (2017) Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides. Cell Proliferation. 50 (4): e12357.

Paino, F., Noce, M.L., Giuliani, A., de Rosa, A., Mazzoni, F., Laino, L., Amler, E., Papaccio, G., Desiderio, V., Tirino, V.: (2017) Human DPSCs fabricate vascularized woven bone tissue: A new tool in bone tissue engineering. Clinical science. 131(8): 699-713.

Rampichová, M., Buzgo, M., Míčková, A., Vocetková, K., Sovková, V., Lukášová, V., Filová, E., Rustichelli, F., Amler, E.: (2017) Platelet-functionalized three-dimensional polye-epsilon-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors. International Journal of Nanomedicine. 12:347-361.

Rampichová, M. , Chvojka, J., Jenčová, V., Kubíková, T., Tonar, Z., Erben, J., Buzgo, M., Daňková, J., Litvinec, A., Vocetková, K., Plencner, M., Prosecká, E., Sovková, V., Lukášová, V., Králíčková, M., Lukáš, D., Amler, E.: (2017) The combination of nanofibrous and microfibrous materials for enhancement of cell infiltration and in vivo bone tissue formation. Biomedical Materials. IN PRESS

Rampichová, M. , Kuželová Košťáková, E., Filová, E., Chvojka, J., Šafka, J., Pelcl, M., Daňková, J., Prosecká, E., Buzgo, M., Plencner, M., Lukáš, D., Amler, E.: (2017) Composite 3D printed scaffold with structured electrospun nanofibers promotes chondrocyte adhesion and infiltration. Cell Adhesion and Migration. Nov 13:1-15. doi: 10.1080/19336918.2017.1385713. [Epub ahead of print]

Sovková, V., Vocetková, K., Rampichová, M., Míčková, A., Buzgo, M., Lukášová, V., Daňková, J., Filová, E. , Nečas, A., Amler, E.: (2017) Platelet lysate as a serum replacement for skin cell culture on biomimetic PCL nanofibers. Platelets. Jun 26:1-11. doi: 10.1080/09537104.2017.1316838. [Epub ahead of print]

Szöke, K., Daňková, J., Buzgo, M., Amler, E., Brinchmann, J.E., Østrup, E.: (2017) The effect of medium composition on deposition of collagen type 1 and expression of osteogenic genes in mesenchymal stem cells derived from human adipose tissue and bone marrow. Process Biochemistry. 59(B): 321-328.

Tejral, G., Sopko, B., Nečas, A., Schoner, W., Amler, E.: (2017) Computer modelling reveals new conformers of the ATP binding loop of Na+/K+-ATPase involved in the transphosphorylation process of the sodium pump. PeerJ. 5: 3087.

Vocetková, K. , Buzgo, M., Sovková, V., Rampichová, M., Staffa, A., Filová, E., Lukášová, V., Doupnik, M., Fiori, F., Amler, E.: (2017) A comparison of high throughput core–shell 2D electrospinning and 3D centrifugal spinning techniques to produce platelet lyophilisate-loaded fibrous scaffolds and their effects on skin cells. RSC Advances. 7(85): 53706-53719.

Vysloužilová, L., Buzgo, M., Pokorný, P., Chvojka, J., Míčková, A., Rampichová, M., Kula, J., Pejchar, K., Bílek, M., Lukáš, D., Amler, E.: (2017) Needleless coaxial electrospinning: A novel approach to mass production of coaxial nanofibers. International Journal of Pharmaceutics. 516(1-2):293-300.

2016

Smyslová, P., Popa, I., Lyčka, A., Tejral, G., Hlaváč, J.:(2016) Non-Catalyzed Click Reactions of ADIBO Derivatives with 5-Methyluridine Azides and Conformational Study of the Resulting Triazoles. PLoS One., 10(12): e0144613.

Filová, E., Jakubcová, B. , Danilová, I., Kuželová Košťáková, E., Jarošíková, T., Chernyavskiy, O., Hejda, J., Handl, M., Beznoská, J., Nečas, A., Rosina, J., Amler, E.: (2016) Polycaprolactone foam functionalized with chitosan microparticles - a suitable scaffold for cartilage regeneration. Physiol. Res., 65(1): 121-131.

Vocetková, K., Buzgo, M., Sovková, V., Bezděková, D. , Kneppo, P., Amler, E.: (2016) Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells. Cell Prolif., 49(5):568-78.

2015

Buzgo, M., Greplová, J., Soural, M., Bezděková, D., Míčková, A., Kofroňová, O., Benada, O., Hlaváč, J., Amler, E.: (2015) PVA immunonanofibers with controlled decay. Polymer. 7: 387-398.

Daňková, J., Buzgo, M., Vejpravová, J., Kubíčková, S., Sovková, V., Vysloužilová, L., Mantlíková, A., Nečas, A., Amler, E.: Highly efficient mesenchymal stem cell proliferation on poly-ε-caprolactone nanofibers with embedded magnetic nanoparticles. Int J Nanomedicine. 10:7307-17.

Erben, J., Pilarová, K., Sanetrnik, F., Chvojka, J., Jenčová, V., Blažková, L., Havlíček, J., Novák, O., Mikeš, P., Prosecká, E., Lukaš, D., Kuzelová Kostaková E.: (2015) The combination of meltblown and electrospinning for bone tissue engineering. Materials Letters 143, 172-176.

Filová, E., Jakubcová, B., Danilová, I., Kuželová Košťáková, E., Jarošíková, T., Chernyavskiy, O., Hejda, J., Handl, M., Beznoská, J., Nečas, A., Rosina, J., Amler, E.: (2015) Polycaprolactone foam functionalized with chitosan microparticles - a suitable scaffold for cartilage regeneration. Physiol Res. IN PRESS

Kubíková, T., Filová, E., Prosecká, E., Plencner, M., Králíčková, M., Tonar, Z.: (2015) Histological evaluation of biomaterials administration in vivo on the cartilage, bone and skin healing. Cas Lek Cesk., 154(3):110-4.

Plencner, M., Prosecká, E., Rampichová, M., East, B., Buzgo, M., Vysloužilová, L., Hoch, J., Amler, E.: (2015) Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution. Int J Nanomedicine.10:2635-2646.

Prosecká, E., Rampichová, M., Litvinec, A., Tonar, Z., Králíčková, M., Vojtová, L., Kochová, P., Plencner, M., Buzgo, M., Míčková, A., Jančář, J., Amler, E.: (2015) Collagen/hydroxyapatite scaffold enriched with polycaprolactone nanofibers, thrombocyte-rich solution and mesenchymal stem cells promotes regeneration in large bone defect in vivo. J. Biomed. Mater. Res. Part A., 103(2): 671-682.

Sukhoruková, I.V., Sheveyko, A.N., Kiryukhantsev-Korneev,Ph.V., AnisimováN.Y., Gloushanková, N.A., Zhitnyak, I.Y., Benešová, J., Amler, E., Shtanský, D.V.: (2015) Two approaches to form antibacterial surface: Doping with bactericidal element and drug loading. Applied Surface Science. 330:339–350.

2014

Amler, E., Filová, E., Buzgo, M., Prosecká, E., Rampichová, M., Nečas, A., Nooeaid, P., Boccaccini, A. R.: (2014) Functionalized nanofibers as drug-delivery systems for osteochondral regeneration. Nanomedicine-UK 9(7): 1083-1094.

Fedorová, P., Srnec, R., Pěnčík, J., Schmid, P., Amler, E., Urbanová, L., Nečas, A.: (2014) Mechanical testing of newly developed biomaterial designed for intra-articular reinforcement of partially ruptured cranial cruciate ligament: ex vivo pig model. Acta Vet.BRNO 83(1): 55-60.

Plencner, M., East, B., Tonar, Z., Otáhal, M., Prosecká, E., Rampichová, M., Krejčí, T., Litvinec, A., Buzgo, M., Míčková, A., Nečas, A., Hoch, J., Amler, E.: (2014) Abdominal closure reinforcement by using polypropylene mesh functionalized with poly-ε-caprolactone nanofibers and growth factors for prevention of incisional hernia formation. Int. J. Nanomed. 9: 3263-3277.

Rampichová, M., Buzgo, M., Chvojka, J., Prosecká, E., Kofroňová, O., Amler, E.: (2014) Cell penetration to nanofibrous scaffolds: Forcespinning®, an alternative approach for fabricating 3D nanofibers. Celll Adhes. Migr. 8(1): 36-41.

2013

Amler, E., Míčková, A., Buzgo, M.: (2013) Electrospun core/shell nanofibers: a promising system for cartilage and tissue engineering? Nanomedicine-UK. 8(4): 509-512.

Buzgo, M., Jakubová, R., Míčková, A., Rampichová, M., Prosecká, E., Kochová, P., Lukas, D., Amler, E.: (2013) Time-regulated drug delivery system based on coaxially incorporated platelet alpha granules for biomedical use. Nanomedicine-UK. 8(7): 1137-1154.

Filová, E., Rampichová M., Litvinec, A., Držík, M., Míčková, A., Buzgo, M., Košťáková, E., Martinová, L., Usvald, D., Prosecká, E., Uhlík, J., Motlík, J., Vajner, L., Amler, E.: (2013) A cell-free nanofiber composite scaffold regenerated osteochondral defects in miniature pigs. Int. J. Pharm. 447(1-2): 139-149.

Rampichová, M., Chvojka, J., Buzgo, M., Prosecká, E., Mikeš, P., Vysloužilová, L., Tvrdik, D., Kochová, P., Gregor, T., Lukáš, D.,Amler, E.: (2013) Elastic three-dimensional poly (ε-caprolactone) nanofibre scaffold enhanced migration, proliferation, and osteogenic differentiation of mesenchymal stem cells. Cell Prolif. 46(1): 23-37.

Czech Science Foundation, grant No. 16-14758S (2016-2019) Influence of surface nanotopography on bioactive properties of low modulus titanium alloy

Ministry of Education Youth and Sports of the Czech Republic, Grant No. LO1309 (1.7.2014 – 30.6.2019) Cell Therapy and Tissue Repair

Ministry of Education Youth and Sports of the Czech Republic, Grant No. LO1508 (NANOGEN) (1.7.2015 – 30.6.2020) Genomics and Proteomics for study of mechanism of biological effect of manufactured nanoparticles

Grant Agency of Charles University of Prague, grant No. 512216 (2016-2019) 3D scaffolds prepared using centrifugal spinning for cartilage and bone regeneration

Grantová agentura České republiky 18-09306S (2018-2020) Vytvoření pokročilých 3D in vitro modelů osteoporózy a zkoumání mechanizmu osteointegrace biomateriálů pro kostní regeneraci 

Ministerstvo průmyslu a obchodu České republiky FV30086 (2018-2021) Kryty ran s antioxidační a antibakteriální funkcí pro hojení chronických ran

University Centre for Energy Efficient Buildings, Czech Technical University in Prague

Faculty of Mechanical Engineering, Czech Technical University in Prague

Faculty of Chemical Technology, University of Chemistry and Technology in Prague

Brno University of Technology, Faculty of Chemistry, Institute of Materials Science

Faculty of Medicine in Plzen, Charles University

Biomedical Center of the Faculty of Medicine in Pilsen

CEITEC – Central European Institute of Technology, Advanced Polymers and Composites

Pavol Jozef Šafárik University in Košice, Faculty of Medicine, Institute of Medical Biophysics

Second University of Naples, Department of Experimental Medicine, Naples, Italy

Mail room:
building La 2. floor, room number 2.18
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Data box:
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Contacts

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+420 241 062 230