Institute of Experimental Medicine CAS

A green tea polyphenol epigallocatechin-3-gallate enhances neuroregeneration after spinal cord injury by altering levels of inflammatory cytokines.

The beneficial effect of EGCG on recovery from SCI was evaluated. EGCG treated rats displayed a superior behavioral performance in flat beam test, higher axonal sprouting and positive remodelation of glial scar. Treatment with EGCG also attenuate canonical NFkB pathway and modulate levels of inflammatory cytokines.

Collaboration: N.Y. M.C, Valhala, USA, prof Meena Jhanwar-Uniyal

Contact person: Machová-Urdziková, 241062619, urdzikl@saske.sk

Publication:
Machová-Urdzíková, L., Růžička, J., Kárová, K., Kloudová, A., Svobodová,
B., Anubhav, A., Dubišová, J., Schmidt, M., Kubinová, Š., Jhanwar Uniyal, M., Jendelová, P.: (2017) A green tea polyphenol epigallocatechin-3-gallate enhances neuroregeneration after spinal cord injury by altering levels of inflammatory cytokines. Neuropharmacology. 126: 213-223.

vav-jendelova-2018

The image A shows transveral section of the spinal cord lesion stained with hematoxyline and NF-κB - DAB staining after EGCG treatment. The nuclear translocation of subunit p65 (RelA) of the NF-κB dimer (black arrows), nuclei stained with hematoxylin without NF- B (red arrow) and cells with NF-B in cell cytoplasm (green arrows). Bar A = 500 μm, Bar A1 = 20 μm. EGCG treatment significantly supports axonal sprouting in spinal cord lesion (B). Locomotor improvement (flat beam test) was observed in animals treated with EGCG when compared with control (C). Levels of proinflammatory cytokines 1 and 3 days after SCI in EGCG and saline-treated groups (D).