Platelet-functionalized three-dimensional poly-Ɛ-epsilon-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors.
PCL three-dimensional fibrous meshes prepared via centrifugal spinning were combined with adhered platelets of five different concentrations. Released growth factors supported cell proliferation and metabolic activity of MG-63 cells in concentrations higher than physiological (300×109/L). Lower concentrations of platelets were not supportive and were comparable to control. Similarly, alkaline phosphatase activity was increased on samples with two most concentrated platelets concentrations.
Mgr. Michala Rampichová, Ph.D., tel.: 296 442 387, firstname.lastname@example.org
University Center of Energy Efficient Buildings, Czech Technical University in Prague, Buštěhrad
Rampichová, M., Buzgo, M., Míčková, A., Vocetková, K., Sovková, V., Lukášová, V., Filová, E., Rustichelli, F., Amler, E.: (2017) Platelet-functionalized three-dimensional polye-epsilon-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors. International Journal of Nanomedicine. 12:347-361.
SEM visualization of platelet adhesion on PCL fibers. Platelets were partially activated and formed a fibrin net 24 h after adhesion (A). Platelets were visibly adhered on fibers even after 14 days of the experiment (B).