Institute of Experimental Medicine CAS

Platelet-functionalized three-dimensional poly-Ɛ-epsilon-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors.

PCL three-dimensional fibrous meshes prepared via centrifugal spinning were combined with adhered platelets of five different concentrations. Released growth factors supported cell proliferation and metabolic activity of MG-63 cells in concentrations higher than physiological (300×109/L). Lower concentrations of platelets were not supportive and were comparable to control. Similarly, alkaline phosphatase activity was increased on samples with two most concentrated platelets concentrations.

Contact person:  Mgr. Michala Rampichová, Ph.D., tel.: 296 442 387, m.rampichova@biomed.cas.cz

Collaboration: University Center of Energy Efficient Buildings, Czech Technical University in Prague, Buštěhrad

Publication:
Rampichová, M., Buzgo, M., Míčková, A., Vocetková, K., Sovková, V., Lukášová, V., Filová, E., Rustichelli, F., Amler, E.: (2017) Platelet-functionalized three-dimensional polye-epsilon-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors. International Journal of Nanomedicine. 12:347-361. 

filova-fig-rampichova-2017

SEM visualization of platelet adhesion on PCL fibers. Platelets were partially activated and formed a fibrin net 24 h after adhesion (A). Platelets were visibly adhered on fibers even after 14 days of the experiment (B).