We appreciate your interest in studying and working at the Institute of Experimental Medicine of the Czech Academy of Sciences.
We are giving importance to the selection of new students and staff because we realize that our success and the great results of our research depend on the skills and experience of everyone. In addition, we realize that the right choice of study and employment is one of the most important decisions in our lives.
We are looking for ambitious, well-motivated, and talented students, who are interested in science. If you have the passion to make new discoveries, the drive to test new theories, and the willingness to go above and beyond, then you are just what we are looking for:)
We believe that your experience and knowledge will benefit our entire team.
We are looking forward to hearing from you! Please complete the application form. You can apply for up to 3 PhD positions.
Department of Developmental Biology
We use knock-out technologies in mice and zebrafish for studying the role of cranial neural crest cells during the embryonic development of head structures. This project will focus on the function of the Wnt/planar cell polarity pathway during neural crest cell differentiation, mesenchymal condensation and bone formation. The conditional knock-out approach and pehnotypical analysis in the mice will be supplemented with explant cultures of embryonic tissues.
Department of Cell Nucleus Plasticity
The emergence of the nucleus is a major evolutionary event that discriminated eukaryotes from prokaryotes. It is a hallmark of nearly all eukaryotic cells and central to their function. Aberrations in the nuclear composition and structure are linked to diseases such as cancer. The project is focused on how cells build a nucleus from different genomic substrates and what is the consequence of nuclear dysregulation; with the ultimate goal of elucidating the function of the nucleus as an essential cellular structure. While many theories exist, it is extremely difficult to assess these in tissue culture cells. Early embryos offer a unique opportunity to study these phenomena: Oocytes build a nucleus from a practically naked DNA (sperm), and also two morphologically and functionally different nuclei (maternal and paternal) co-exist in a common cytoplasm after fertilization. In contrast to egg extracts, this system offers an unprecedented chance to rigorously test the consequence of targeted nuclear dysregulation through the ability of embryos to give rise to live animals.
Department of Cellular Neurophysiology
The activation of glia as a reaction to brain tissue damage during ischemia or epileptic seizure results in an overexpression of extracellular matrix (ECM) molecules; these accumulate in the extracellular space and, together with the morphological changes of glial cells, affect its diffusion properties and thus extrasynaptic transmission. The presence of an aberrant ECM may not only lead to changes in the volume and tortuosity of the extracellular space, but may also shape post-ischemic tissue regeneration and/or epileptogenesis through the complex interplay between the ECM, astrocytes, and neurons; this could be an important factor in pathogenic plasticity. To evaluate the quantitative/qualitative ECM changes and the role of the distinct types of glial cells in post-ischemic and epileptic tissue with regards to brain diffusion, laboratory techniques, such as the real-time iontophoretic method, imunofluorescence, Western blot, fluorescence-activated cell sorting and RNA-Seq analysis, will be utilized in experimental animal models of transient ischemia and epilepsy.
Department of Neurochemistry
N-methyl-D-aspartate receptors (NMDARs) are a subclass of glutamate receptors that play an essential role in excitatory neurotransmission in the mammalian brain. NMDARs are tetrameric protein complexes composed of GluN1, GluN2, and GluN3 subunits, which can assemble in a variety of combinations. In this Ph.D. project, you will combine state-of-the-art molecular biology, biochemistry, immunocytochemistry and electrophysiology, with unique mice models in order to identify: i) which structural determinants in GluN subunits and NMDAR ligands regulate the surface delivery, mobility, and stability of NMDARs; and ii) how specific NMDARs and their ligands contribute to neurodegenerative conditions.
Department of Tissue Engineering
Materials for wound healing should regenerate the full thickness defects and restore the tissue function. They should support cell growth, production of extracellular matrix, vascularization, and prevent bacterial contamination. The scaffolds should mimic the structure of the tissue and should deliver bioactive substances for the enhancing of regeneration. The scaffolds will be developed as two-dimensional and three-dimensional systems delivering growth factors or other bioactive molecules, and will be tested with skin cells and stem cells. Moreover, a 3D in vitro model of both skin and intestinal tissues will be established for toxicological screening. This topic is being studied among international consortium of H2020 MSCA RISE project ActiTOX, therefore the applicant will have opportunities for secondments in other laboratories in the EU.
Department of Nanotoxicology and Molecular Epidemiology
Although nanoparticles (NP) are widely used in numerous industrial and medical products, there is still limited knowledge on their potential impact on human health. Experimental studies indicate that NP may cause damage to genetic material and negatively affect cellular functions, although the biological effects depend on their physico-chemical characteristics. Stem cells that play an important role in regeneration and wound healing may be particularly sensitive to the deleterious effects of NP. The aim of the project is to investigate the impacts of metal NP with antimicrobial properties, on the processes and function characteristics of mouse mesenchymal stem cells (MSC). Among other parameters, the induction of DNA damage, oxidative stress, apoptosis, cell cycle changes, as well as global mRNA and miRNA expression, will be investigated in MSC treated in vitro with Ag, CuO and ZnO NP. The results will be further evaluated in vivo in mouse experimental models, in which the effects of NP on the processes of wound healing will be investigated.
Organization of the PhD studies
The study is provided by the contracting universities, including Charles University in Prague, the Czech Technical University in Prague, the University of Chemistry and Technology in Prague, and others.
The main role in planning and organizing the study is played by the supervisor from IEM CAS, in cooperation with the relevant university.
The conditions of admission and the organization of studies, are governed by the valid regulations of the particular university.
Detailed information will be provided by your Supervisor.
For general questions about PhD studies, please contact our PhD Coordinator firstname.lastname@example.org
About the Institute of Experimental Medicine CAS
The Institute of Experimental Medicine (IEM CAS), is a recognized center of basic biomedical research in the Czech Republic. At present, the institute consists of 11 separate scientific departments, focusing on research in biochemistry, cell and developmental biology, pathology, molecular embryology, genetic toxicology and nanotoxicology, neurobiology, neurophysiology, neuropathology, oncology, tissue replacement, and nanomedicine.
We develop and verify analytical, diagnostic, and therapeutic methods.
The research program at IEM CAS covers current trends in the field and enables interdisciplinary approaches to solving serious issues in biomedicine. Researchers of the institute actively cooperate with a number of important national and international partners. The results of research carried out at the institute are already applied in the field of environmental protection, neuroscience, regenerative medicine, pharmacology and diagnostic methods.
You can find more information on the official website www.iem.cas.cz/en