Institute of Experimental Medicine CAS

Published 07. 05. 2021

Researchers from the Department of Tissue Engineering have recently published two studies examining the effect of different types of substances that promote the growth and survival of stem cells and connective tissue cells.

The first article, published in March 2021 in the journal Biomolecules, is the study The Effect of Alendronate on Osteoclastogenesis in Different Combinations of M-CSF and RANKL Growth Factors.

In this study, the effect of different concentrations of Alendronate, a drug for the treatment of osteoporosis, on the formation and proper function of osteoclasts, i.e. cells degrading bone, was observed. The effect was examined in the presence of different combinations of growth factors necessary for osteoklast formation. The study was carried out on rat and human cells. It was found out that the highest Alendronate concentration (10^-6 M) had a cytotoxic effect, but the lower concentrations (10^-7 – 10^-9 M) did not exert any effect on osteoclast formation and function. Moreover, differences in morphology, proliferation, metabolic activity and expression of osteoclast markers were observed between the rat and human cells.

A second study was published in the International Journal of Molecular Sciences last week,with the title Cellular Response to Individual Components of the Platelet Concentrate.

In it, the researchers focused on a more detailed study of the platelet lysate and its components, which are used to cultivate cells instead of serum. Mesenchymal stem cells and fibroblasts were cultured in the presence of platelet proteins in plasma, pure platelet proteins or pure plasma. The results indicated that the highest cell viability was achieved by the use of platelet proteins and plasma. Contrary to that, the lowest cell viability was observed in the presence of pure platelet proteins. Plasma alone supported the cell viability in the same way as normally used serum.

Link to the published articles:

The Effect of Alendronate on Osteoclastogenesis in Different Combinations of M-CSF and RANKL Growth Factors.
https://www.mdpi.com/2218-273X/11/3/438

Cellular Response to Individual Components of the Platelet Concentrate
https://www.mdpi.com/1422-0067/22/9/4539