Institute of Experimental Medicine CAS

Colorectal adenoma, a protuberance from the epithelium of the intestine, is a precursor of colorectal cancer, the third most common cancer worldwide. Since the molecular mechanisms behind the early transformation into an adenoma are not well understood, we aimed to identify novel candidate genes by whole transcriptomic sequencing (WTS) of a larger cohort of adenomas and their corresponding adjacent tissue. The analysis revealed more than 2,000 differentially expressed genes (DEGs) between the adenoma and the adjacent tissue. Functional consequences of the gene signature were analyzed by proliferation assay, immunohistochemistry and in vivo in the chorioallantoic membrane (CAM) assay.