
Head:
Helena Fulková, Ph.D.
E-mail:
helena.fulkova@iem.cas.cz
Phone: +420 241 062 232
+420 241 062 588
Twitter:
@FulkaLab
E-mail:
helena.fulkova@iem.cas.cz
Phone: +420 241 062 232
+420 241 062 588
Twitter:
@FulkaLab
The group is focused on studying the cell nucleus plasticity namely during early mammalian embryogenesis. The process of differentiation and de-differentiation is accompanied by dramatic changes of the nucleus morphology and epigenetic remodelling of chromatin. The aim of our group is to characterize these changes and to define the role of specific sub-nuclear organelles in the process.
Zuzana Smékalová, MSc.
zuzana.smekalova@iem.cas.cz
Phone: +420 241 062 232
Pavel Čabart, PhD
pavel.cabart@iem.cas.cz
Oksana Shcherbak, PhD
oksana.shcherbak@iem.cas.cz
Tereza Znachorová, MSc.
tereza.znachorova@iem.cas.cz
Nataliia Dudko, MSc.
nataliia.dudko@iem.cas.cz
Rychtarova, J., Langerova, A., Fulka, H., Loi, P., Benc, M., Fulka, J, Jr.: (2021) Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report. Animals. 2021; 11(5):1250. https://doi.org/10.3390/ani11051250
Chapter in: Palermo, G., & Nagy, Z. (Eds.). (2021). Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction: With Other Advanced Micromanipulation Techniques to Edit the Genetic and Cytoplasmic Content of the Oocyte. Cambridge: Cambridge University Press. https://doi.org/10.1017/9781108887595
Publication:
Loubalova, Z., Fulka, H., Horvat, F., Pasulka, J., Malik, R., Hirose, M., Ogura, A., Svoboda, P.: (2021) Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs. Nature Cell Biology. 2021; 23(9): 992-1001. doi: 10.1038/s41556-021-00746-2 – In collaboration with IMG a RIKEN BRC, Japan
Article:
Hirose, M., Honda, A., Fulka, H. et al.: (2020) Acrosin is essential for sperm penetration through the zona pellucida in hamsters, Proceedings of the National Academy of Sciences of the United States of America, 117,5,2513-2518
Somatic cell nucleus transfer experiments show that the oocyte is capable of remodelling the transferred nucleus to such an extent that a new individual can be born. However, what oocyte components are responsible for the process is not entirely clear. Using the selective enucleation we have shown that it is the soluble nuclear fraction, which has the biggest effect on the transferred nucleus. The selective enucleation will allow us to study the replication dependent and independent processes of nuclear reprogramming. The article was selected as an “Outstanding paper 2019” and got a cover of the October 2019 issue of Journal of Reproduction and Development.
Article:
Fulka, H., Ogura, A., Loi, P., et al.: (2019) Dissecting the role of the germinal vesicle nuclear envelope and soluble content in the process of somatic cell remodelling and reprogramming, Journal of Reproduction and Development, 65,5,433-441
https://www.jstage.jst.go.jp/article/jrd/65/5/65_2019-017/_article
Fulka, H ., Loi, P., Czernik, M., Surani, A., Fulka, J. Jr.: Omne vivum ex ovo: the oocyte reprogramming and remodeling activities. Reproduction 2023. 165 (3). R75–R89. doi: https://doi.org/10.1530/REP-22-0124
2021
Loubalova, Z., Fulka, H., Horvat, F., Pasulka, J., Malik, R., Hirose, M., Ogura, A., Svoboda, P.: Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs. Nature Cell Biology. 2021; 23(9): 992-1001. doi: 10.1038/s41556-021-00746-2 - In collaboration with IMG and RIKEN BRC
Dzijak, R., Galeta, J., Vázquez, A., Kozák, J., Matoušová, M., Fulka, H., Dračínský, M., Vrabel, M.: Structurally Redesigned Bioorthogonal Reagents for Mitochondria-Specific Prodrug Activation. JACS Au. 2021 Jan 25;1(1):23-30. doi: 10.1021/jacsau.0c00053. Epub 2020 Dec 15. PMID: 33554213; PMCID: PMC7851953.
Rychtarova, J., Langerova, A., Fulka, H., Loi, P., Benc, M., Fulka, J, Jr.: Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report. Animals. 2021; 11(5):1250. https://doi.org/10.3390/ani11051250
Chapter in a textbook: Palermo, G., & Nagy, Z. (Eds.). Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction: With Other Advanced Micromanipulation Techniques to Edit the Genetic and Cytoplasmic Content of the Oocyte. 2021. Cambridge: Cambridge University Press. doi:10.1017/9781108887595 https://doi.org/10.1017/9781108887595
Benc, M., Martinkova, S., Rychtarova, J., Fulka, J., Bartkova, A., Fulka, H., Laurincik, J.: Assessing the effect of interspecies oocyte nucleolar material dosage on embryonic development, Theriogenology, 2020. DOI: 10.1016/j.theriogenology.2020.06.001
Fulka, H. , Rychtarova, J., Loi, P.: The nucleolus-like and precursor bodies of mammalian oocytes and embryos and their possible role in post-fertilization centromere remodelling, Biochemical Society Transactions, 2020. 48,581-593
Hirose, M., Honda, A., Fulka, H. et al.: Acrosin is essential for sperm penetration through the zona pellucida in hamsters, Proceedings of the National Academy of Sciences of the United States of America, 2020. 117,5,2513-2518
GAČR 20-04465S (1. 1. 2020 – 31. 12. 2022)
Understanding the factors critical for successful paternal genome remodelling: Dissecting the role of oocyte components
GAČR 21-42225L (2021-2024)
Genome stability in mammalian oocytes and somatic cells
Molecular and functional analysis of the nucleolus in 3D genome organization during early embryo development
prof. Jerzy Dobrucki, PhD, DSc
Department of Cell Biophysics
Jagiellonian University of Krakow
Krakow, Poland
Prof. Pasqualino Loi
Faculty of Veterinary Medicine, Laboratory of Embryology
University of Teramo
Teramo, Italy
Dr. Milan Vrábel
Chemistry of Bioconjugates
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences
Prague, Czech Republic
Dr. Tomáš Etrych
Biomedical Polymers
Institute of Macromolecular Chemistry CAS
Prague, Czech Republic
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