We study the lateral organization of biological membranes into microdomains, i.e., areas with specific shape, composition and function. We put the emphasis on their involvement in the regulation of cellular processes in response to stimuli from the environment. Taking the maximum advantage of the genetically accessible yeast model, we particularly investigate the role of membrane microdomains in stress perception and adaptation, in signaling and regulation of cellular metabolism. These membrane functions presuppose communication between different microdomains, both within one particular membrane and between different specialized membranes within the membrane system of a eukaryotic cell. For example, we have recently described how a protein native to plasma membrane domains affects the morphology and functional properties of microdomains in the membrane of the vacuole.
Deputy Head:
Ing. Petra Veselá
E-mail: petra.vesela@iem.cas.cz
Tel.: +420 241 062 119
Research Scientists:
Assoc. Prof. Jan Malínský, Ph.D.
Jakub Zahumenský, MSc, Ph.D.
Research Assistant:
Ing. Petra Veselá
PhD. Students:
Ing. Petra Veselá
Satyendra Mondal, M.Sc.
Alena E. Shulutkova, M.Sc.
Technicans:
Jitka Eisensteinová
Lenka Hlavínová (ML)
Important results in 2020
Plasma membrane protein Nce102 modulates morphology and function of the yeast vacuole
A putative plasma membrane sphingolipid sensor Nce102 migrates to the vacuole upon gradual ageing of a yeast culture, when sphingolipid demand increases. Within the vacuolar membrane it stably localizes to V-ATPase-poor, i.e., ergosterol-enriched, domains, analogous to its plasma membrane localization. Together with its homologue Fhn1, Nce102 modulates size and shape of membrane domains, dynamics of vacuole fusion, and stability of the V-ATPase.
Increasing Nce102 amount and vacuole localization with culture age. Bar: 5µm (A); Changes in vacuole morphology in nce102Δ and fhn1Δ mutants. Bar: 5µm (B);Western blot analysis of increased degradation of Vph1-GFP (V-ATPase subunit) in nce102Δ, fhn1Δ and nce102Δfhn1Δ (C); Electron micrograph of a freeze-fracture replica with proposed trajectory of Nce102 protein migration in an ageing culture. Bar: 300 nm (D).
Publication: Vaškovičová K., Veselá P., ZahumenskýJ., Folková, D., Balážová M., Malínský J.: (2020) Plasma Membrane Protein Nce102 Modulates Morphology and Function of the Yeast Vacuole. Biomolecules, 10(11), 1476; doi: 10.3390/biom10111476. IF 4.694
Important results in 2017
Regulation of mRNA decay at the plasma membrane
Evolutionarily conserved 5´-3´exoribonuclease Xrn1 binds to eisosome
under conditions of glucose deprivation. Association of Xrn1 with the plasma membrane represents a novel mechanism of the mRNA decay regulation. Subcellular Xrn1 localization also reflects the nutrient supply and metabolic differentiation within the yeast colony. Central section of the whole 5 days old colony of S. cerevisiae expressing Xrn1-GFP (A), stratification of specific Xrn1-GFP patterns (B), and large magnification details (C) of cells showing Xrn1 distributed in cytosol (1), accumulated in P-bodies (2) and associated with eisosome (3) are presented. Fluorescence Recovery After Photobleaching (FRAP) analysis revealed that in contrast to P-body binding, the enzyme association with the plasma membrane at eisosome is stable in time (D). Bars: 300 μm (A), 10 μm (C).
Publication:
Vaškovičová K., Awadová T., Veselá P. , Balážová M., Opekarová M., Malínský J.: (2017) mRNA decay is regulated via the spatial sequestration of the conserved 5 '-3 ' exoribonuclease Xrn1 at a specific microdomain of the yeast plasma membrane. European Journal of Cell Biology. 96(6): 591-599. IF 3,712.
2023
Filipi T, Matusova Z, Abaffy P, Vanatko O, Tureckova J, Benesova S, Kubiskova M, Kirdajova D, Zahumensky J, Valihrach L, Anderova M. Cortical glia in SOD1(G93A) mice are subtly affected by ALS-like pathology. Scientific Reports 2023.
Vesela P , Zahumensky J, Malinsky J. Lsp1 partially substitutes for Pil1 function in eisosome assembly under stress conditions. J Cell Sci. 2023 Feb 1;136(3):jcs260554. doi: 10.1242/jcs.260554. Epub 2023 Feb 6. PMID: 36601791.
2022
Balazova M, Babelova L, Durisova I, Vesela P, Kanovicova P, Zahumensky J, Malinsky J. Two different phospholipases C, Isc1 and Pgc1, cooperate to regulate mitochondrial function. bioRxiv preprint doi: https://doi.org/10.1101/2022.03.18.484854; this version posted March 18, 2022.
Zahumenský J ,Mota Fernandes C, Veselá P, Del Poeta M, Konopka JB, Malínský J. Microdomain Protein Nce102 Is a Local Sensor of Plasma Membrane Sphingolipid Balance. Microbiol Spectr. 2022 Jun 27:e0196122. doi: 10.1128/spectrum.01961-22. Epub ahead of print. PMID: 35758748.
Káňovičová P, Čermáková P, Kubalová D, Bábelová L, Veselá P, Valachovič M, Zahumenský J, Horváth A, Malínský J, Balážová M. Blocking phosphatidylglycerol degradation in yeast defective in cardiolipin remodeling results in a new model of the Barth syndrome cellular phenotype. J Biol Chem. 2022 Jan;298(1):101462. doi: 10.1016/j.jbc.2021.101462. Epub 2021 Dec 2. PMID: 34864056; PMCID: PMC8728584.
2020
Vaskovicova K , Vesela P, Zahumensky J, Folkova D, Balazova M, Malinsky J. Plasma Membrane Protein Nce102 Modulates Morphology and Function of the Yeast Vacuole. Biomolecules. 2020 Oct 23;10(11):E1476. doi: 10.3390/biom10111476. PMID: 33114062.
Chen Y, Zhao G, Zahumensky J, Honey S, Futcher B. Differential Scaling of Gene Expression with Cell Size May Explain Size Control in Budding Yeast. Mol Cell. 2020;78(2):359-370.e6. doi:10.1016/j.molcel.2020.03.012
2019
Zahumensky J, Malinsky J .:(2019) Role of MCC/Eisosome in Fungal Lipid Homeostasis. Biomolecules. 2019 Jul 25;9(8). pii: E305. doi: 0.3390/biom9080305. Review.
Kubalová D, Káňovičová P, Veselá P, Awadová T, Džugasová V, Daum G, Malínský J, Balážová M.:(2019) The lipid droplet protein Pgc1 controls the subcellular distribution of phosphatidylglycerol. FEMS Yeast Res. 2019 Aug 1;19(5). pii: foz045. doi: 10.1093/femsyr/foz045.
2018
Pivoňková, H. , Heřmanová, Z. , Kirdajová, D. , Awadová, T., Malínský, J., Valihrach, L., Žucha, D., Kubista, M., Gálisová, A., Jirák, D., Anděrová, M.: (2018) The Contribution of TRPV4 Channels to Astrocyte Volume Regulation and Brain Edema Formation. Neuroscience. 2018 Dec 1;394:127-143. doi: 10.1016/j.neuroscience.2018.10.028. Epub 2018 Oct 24.
Awadová, T ., Pivoňková, H., Heřmanová, Z., Kirdajová, D., Anděrová, M., Malínský, J.: (2018) Cell volume changes as revealed by fluorescence microscopy: Global vs local approaches. J Neurosci Methods. 2018 Aug 1;306:38-44. doi: 10.1016/j.jneumeth.2018.05.026. Epub 2018 Jun 7.
2017
Vaškovičová, K., Awadová, T., Veselá, P. , Balážová, M., Opekarová, M., Malinsky, J.: (2017) mRNA decay is regulated via the spatial sequestration of the conserved 5 '-3 ' exoribonuclease Xrn1 at a specific microdomain of the yeast plasma membrane. European Journal of Cell Biology. 96(6): 591-599.
2016
Chum, T., Glatzová, D., Kvíčalová, Z., Malínský, J., Brdička, T., Cebecauer, M.: (2016) The role of palmitoylation and transmembrane domain in sorting of transmembrane adaptor proteins. J. Cell Sci., 129(1): 95-107.
Malinsky, J., Opekarová, M.: (2016) New Insight Into the Roles of Membrane Microdomains in Physiological Activities of Fungal Cells. Int. Rev. Cell Mol. Biol., 325: 119-180.
Malinsky, J., Tanner, W., Opekarová, M.: (2016) Transmembrane voltage: Potential to induce lateral microdomains. Acta Mol. Cell Biol. Lipids, 1861(8 Pt B): 806-811.
Plecitá-Hlavatá, L., Engstová, H., Alán, L., Špaček, T., Dlasková, A., Smolková, K., Špačková, J., Tauber, J., Strádalová, V., Malínský, J., Lessard, M., Bewersdorf, J., Ježek, P.: (2016) Hypoxic HepG2 cell adaptation decreases ATP synthase dimers and ATP production in inflated cristae by mitofilin down-regulation concomitant to MICOS clustering. Faseb J., 30(5): 1941-1957.
Pokorná, L., Čermáková, P., Horváth, A., Baile, M.G., Claypool, S.M., Griač, P., Malínský, J., Balážová, M.: (2016) Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function. Biochim. Biophys. Acta-Bioenerg., 1857(1): 34-45.
Wang, H.X., Douglas, L.M., Veselá, P., Rachel, R., Malinsky, J., Konopka, J.B.: (2016) Eisosomes promote the ability of Sur7 to regulate plasma membrane organization in Candida albicans. Mol Biol Cell., 27(10): 1663-1675.
2015
Grousl, T., Opekarová, M., Strádalová, V., Hašek, J., Malinsky, J.: (2015) Evolutionarily Conserved 5'-3' Exoribonuclease Xrn1 Accumulates at Plasma Membrane-Associated Eisosomes in Post-Diauxic Yeast. PLoS One. 10(3): e0122770.
Herman, P., Večeř, J., Opekarová, M., Veselá, P., Jančíková, I., Zahumenský, J., Malinsky, J.: (2015) Depolarization affects lateral microdomain structure of yeast plasma membrane. FEBS J. 282(3): 419-434.
Pokorná, L., Čermáková, P., Horváth, A., Baile, M.G., Claypool, S.M., Griač, P., Malínský, J., Balážová, M.: (2015) Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function. Biochim Biophys Acta. 1857(1): 34-45.
Vaškovičová, K., Strádalová, V., Efenberk, A., Opekarová, M., Malínský, J. : (2015) Assembly of fission yeast eisosomes in the plasma membrane of budding yeast: Import of foreign membrane microdomains. Eur. J. Cell Biol. 94(1): 1-11.
2014
Vecer, J., Veselá, P., Malínský, J., Herman, P.: (2014) Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast. FEBS Lett. 588(3): 443-449.
Malinsky, J., Opekarová, M. , Grossmann, G., Tanner, W.: (2013) Membrane microdomains, rafts, and detergent-resistant membranes in plants and fungi. Annu. Rev. Plant Biol. 64: 501-529.
Rinnerthaler, M., Slaba, R., Grousl, T., Strádalová, V., Heeren, G., Richter, K., Breitenbach-Koller, H., Malinsky, J., Hasek, J., Breitenbach, M.: (2013) Mmi1, the yeast homologue of mammalian TCTP associates with stress granules in Heat-Shocked Cells and Modulates proteasome activity. PLoS One. 8(10): e77791.
SAV-18-25 Barth syndrome - Study of mitochondrial morphology in a novel yeast model, 2018-2021
19-04052S Function of specific membrane microdomains in lipid homeostasis, 2019-2021
20-04987S The role of eisosome in the regulation of stress response, 2020-2022
Institute of Animal Biochemistry and Genetics SAS
University of Regensburg
Institute of Physics of Charles University
Stony Brook University
Prague Membrane Discussions (PMD)
Informal scientific discussions focused on cellular membranes (not only).
https://praguemembranediscussions.weebly.com/
Spring Event
March 19, 2020 (Thursday)
J. Heyrovsky Institute of Physical Chemistry (JHI) of the Czech Academy of Sciences (Ladvi)
Where:
Brdicka Lecture Hall
9:30 am - 14:00 am
Program
9:30-10:00
Meeting Friends – wake-up discussions, coffee, refreshments.
10:00-11:00
Dominik Wöll, RWTH Aachen University, Germany
“ Functional microgels – new insight into their structure and function with super-resolution fluorescence microscopy ”
11:0-12:00
Christian Franke, Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
“ Elucidating the endosomal machinery with correlative multi-colour SMLM and volumetric electron tomography ”
12:00-12:30
COFFEE/SNACKS BREAK
(drinks, coffee and small snacks available)
12:30-13:15
Piotr Jurkiewicz, JHI
„ When BOBBING does not mean BOBOVÁNÍ ('bobsleigh' in Czech) “
13:15-14:00
Joana Ricardo, JHI
„ Characterization of lipid bilayer nanodiscs “
14:00 ->
General discussions
Spring PMD 2018
March 8th, 2018 ; 10 am - 2 pm
@ Jaroslav Heyrovsky Institute of the CAS, Dolejskova 3, Prague 8
Brdicka lecture room
Program:
10:00 - 11:00 Plenary lecture 1.
Claudia Steinem - Universitaet Goettingen, Germany
Pore-spanning membranes: Is this model system suited for addressing biochemical questions?
11:00 - 12:00 Plenary lecture 2.
Andreas Janshoff - Universitaet Goettingen, Germany
Membrane biophysics in confined geometry
12:00-12:30 COFFEE/SNACKS BREAK (drinks, coffee and snacks available)
12:30 – 13:15
Robert Vacha, MU Brno
Translocation of peptides across phospholipid membranes
13:15 – 14:00
Petra Riegrova, JHI
The oxidized phospholipids modify Bax protein ability to induce permeabilization of mitochondrial membranes
2017
Poslední PMD v roce 2017 se uskutečnilo ve čtvrtek 14. 12. a hostitelem byl tentokrát Ústav experimentální botaniky AV ČR.
Konferenci zahájil doktor Jan Petrášek.
December 14th, 2017; 10 am - 3 pm
@ Institute of Experimental Botany of the CAS, Prague 6 - Lysolaje
Building B1, Ground floor, Library seminar room
Program:
10:00 - 10:30 Charo I. Del Genio
In silico modelling of auxin plasma membrane carrier
Centre for Complexity Science, University of Warvick, UK
10:30 - 11:00 Roman Pleskot
Membrane targeting of Arabidopsis TPLATE complex
Group of Advanced Live Cell Imaging, VIB Center for Plant Systems Biology, Ghent, Belgium
Roman Pleskot, Klaas Yperman, Jie Wang and Daniel Van Damme
11:00-11:20 COFFEE BREAK (drinks, coffee and snacks available)
11:20 – 11:50 Martin Potocký
Phosphatidic acid is involved in clathrin-mediated endocytosis in tip-growing plant cells
Laboratory of Cell Biology, Institute of Experimental Botany CAS
Přemysl Pejchar, Juraj Sekereš, Roman Pleskot, Viktor Žárský and Martin Potocký
11:50-12:20 Mária Balážová
Subcellular distribution of phosphatidylglycerol is regulated by a lipid droplet protein
Department of Membrane Biochemistry, Institute of Animal Biochemistry and Genetics, Centre of Biosciences SAS, Bratislava, Slovakia
12:20-13:30 Lunch - canteen of IEB CAS or pizza at the site
13:30 – 14:00 Radovan Fišer
Interaction of lipopeptide Daptomycin with the membrane and the pore-formation
Laboratory of Bacterial Physiology, Department of Genetics and Microbiology, Faculty of Science, Charles University
14:00 – 14:30 Michal Daněk
Flotillin2 in Arabidopsis or Do membrane microdomains reflect cell wall integrity?
Laboratory of Signal Transduction, Institute of Experimental Botany CAS
Michal Daněk, Jan Petrášek, Jindřiška Angelini, Kateřina Malínská
14:30-15:00 Katarzyna Retzer
SRRF based analysis of the distribution of a mutated variant of the plasma membrane located auxin carrier AtPIN2
Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany CAS
Úvodní přednášku měl Charo del Genio z britske University of Warwick.
...kromě přednášek bylo na akci také dost času pro neformální diskuse a setkání s kolegy.
December 14th, 2017; 10 am - 3 pm
@ Institute of Experimental Botany of the CAS, Prague 6 - Lysolaje
Building B1, Ground floor, Library seminar room
Program:
10:00 - 10:30 Charo I. Del Genio
In silico modelling of auxin plasma membrane carrier
Centre for Complexity Science, University of Warvick, UK
10:30 - 11:00 Roman Pleskot
Membrane targeting of Arabidopsis TPLATE complex
Group of Advanced Live Cell Imaging, VIB Center for Plant Systems Biology, Ghent, Belgium
Roman Pleskot, Klaas Yperman, Jie Wang and Daniel Van Damme
11:00-11:20 COFFEE BREAK (drinks, coffee and snacks available)
11:20 – 11:50 Martin Potocký
Phosphatidic acid is involved in clathrin-mediated endocytosis in tip-growing plant cells
Laboratory of Cell Biology, Institute of Experimental Botany CAS
Přemysl Pejchar, Juraj Sekereš, Roman Pleskot, Viktor Žárský and Martin Potocký
11:50-12:20 Mária Balážová
Subcellular distribution of phosphatidylglycerol is regulated by a lipid droplet protein
Department of Membrane Biochemistry, Institute of Animal Biochemistry and Genetics, Centre of Biosciences SAS, Bratislava, Slovakia
12:20-13:30 Lunch - canteen of IEB CAS or pizza at the site
13:30 – 14:00 Radovan Fišer
Interaction of lipopeptide Daptomycin with the membrane and the pore-formation
Laboratory of Bacterial Physiology, Department of Genetics and Microbiology, Faculty of Science, Charles University
14:00 – 14:30 Michal Daněk
Flotillin2 in Arabidopsis or Do membrane microdomains reflect cell wall integrity?
Laboratory of Signal Transduction, Institute of Experimental Botany CAS
Michal Daněk, Jan Petrášek, Jindřiška Angelini, Kateřina Malínská
14:30-15:00 Katarzyna Retzer
SRRF based analysis of the distribution of a mutated variant of the plasma membrane located auxin carrier AtPIN2
Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany CAS
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