Department of Genetic Toxicology and Epigenetics (DGTE) is focused on the mechanisms of toxic effects of engineered nanoparticles as well as combustion originated fine and ultrafine particles. Major attention is paid to the interaction of particles and their components with biomolecules in human cell lines. Currently, DGTE is focused on the role of ultrafine particles in the etiology of neurodegenerative diseases. The methods include various tests of cytotoxicity, the detection of reactive oxygen species and oxidative damage of DNA, proteins and lipids (ELISA), analyzes of genotoxicity (Comet assay, DNA adducts) and clastogenic effects (micronucleus test and cytogenetic analysis by FISH), as well as analyzes of protein expression (western blotting) and DNA repair activity (NER and NHEJ). To get more insight into the mechanism of biological effects of particles or their extracts, the toxicogenomic methods (microarrays and next generation sequencing) and RT-PCR are used. Most of these tests are also available to external users from research, universities, industry and academia via the Research Infrastructure NanoEnviCz (www.nanoenvicz.cz).
Deputy Head:
Ing. Táňa Závodná, Ph.D.
E-mail: tana.zavodna@iem.cas.cz
Tel.: +420 241 062 663
Research Scientists:
Ing. Jan Topinka, DSc.
Ing. Hana Barošová, Ph.D.
Kateřina Hoňková, Ph.D.
Ing. Zdeněk Krejčík, Ph.D.
Assoc. Prof. Stanislav Pavelka, CSc.
Jitka Sikorová, Ph.D.
Ing. Táňa Závodná, Ph.D.
PhD. Students:
Tereza Červená, MSc.
Jitka Pavlíková, MSc.
Research Assistants:
RNDr. Irena Chvojková
Hasmik Margaryan, MSc.
Alena Milcová, MSc.
Michaela Pokorná, MSc.
Ing. Věra Topinková
Important results in 2020
1. DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles
The risk of human exposure to nanoparticles has rapidly increased during the last decade. We investigated DNA methylation profiles in a group of long-term exposed research workers and in controls. Both groups were sampled twice/day (pre-shift, post-shift). The obtained array data showed significant differences in methylation related to long-term exposure (341 CpG loci hypomethylated and 364 hypermethylated). In contrast, short-term acute exposure was not accompanied by DNA methylation changes.
The impact of the nanoparticle exposure on the DNA methylation pattern. (a) Comparison between exposed pre-shift and post-shift groups. (b) Comparison between control pre-shift and post-shift groups. (c) Comparison between exposed and control pre-shift groups. (d) Comparison between exposed and control post-shift groups.
Publications:
Rossnerova
, A.; Honkova, K.; Pelclova, D.; Zdimal, V.; Hubacek, J.A.; Chvojkova, I.; Vrbova, K.; Rossner, P.; Topinka, J.; Vlckova, S.; et al. DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles. Int. J. Mol. Sci. 2020, 21, 2420.
2. The Molecular Mechanisms of Adaptive Response Related to Environmental Stress
Numerous studies indicate that living organisms possess the ability to adapt to adverse environmental conditions, as illustrated by the fact that DNA damage in populations living in the environment with high levels of air pollution do not correspond to the concentrations of pollutants. The molecular mechanisms, including suggestion of the model of epigenetic adaptation by the DNA methylation pattern modification and its preservation by the process of epigenetic memory, were described.
The model of the process of adaptation to environmental exposure and its “storage” by epigenetic memory (EM) in stem cells.
Different exposure scenarios during the life and their consequences: (a) New adaptation to chronic exposure A and preservation by EM. (b) New adaptation to chronic exposure B and preservation by EM. (c) No adaptation to acute exposure C and no preservation by EM. (d) Already adapted to chronic re-exposure A via EM. (e) Already adapted to acute re-exposure B via EM.
Publications:
Rossnerova, A.; Izzotti, A.; Pulliero, A.; Bast, A.; Rattan, S.I.S.; Rossner, P. The Molecular Mechanisms of Adaptive Response Related to Environmental Stress. Int. J. Mol. Sci. 2020, 21, 1-15.
Important results in 2019
1. Benzo[a]pyrene is associated with dysregulated myelo-lymphoid hematopoiesis in asthmatic children
Exposure to B[a]P might contribute to concurrent suppression of pro-inflammatory (e.g. NF-κB mediated Natural Killer T cells), and activation of anti-inflammatory pathways (e.g. IL10-secreting CD8+ T cells) in the urban asthmatic children. In addition, B[a]P appears to elevate heme biosynthesis, which in turn, promotes neutrophilic metamyelocyte expansion and reduction of CD71+ erythroid cells.
Overview of integrative network analysis
Publications:
Choi, H., Song, WM., Wang, M., Dostal, M., Pastorkova, A., Libalova, H., Tulupova, E., Rossnerova, A., Rossner, P. Jr., Sram, RJ., Zhang, B. (2019): Benzo[a]pyrene is associated with dysregulated myelo-lymphoid hematopoiesis in asthmatic children. Environ Int. 128: 218-232. doi: 10.1016/j.envint.2019.04.052. IF=7.943
2. The biological effects of complete gasoline engine emissions exposure in a 3D human airway model (MucilAirTM) and in human bronchial epithelial cells (BEAS-2B)
The aim of this study was real-time exposure of complete combustion emissions in two cell models. The models used were the BEAS-2B lung epithelial cell line and the MucilAirTM 3D lung model. To allow direct contact of combustion emissions and cells, both models were cultured on porous membranes. A unique exposure system has been created to allow real time direct expose to diluted exhaust gases. One-day and five-day repeated exposure showed higher tolerance and more realistic results in complex 3D models than commonly used lung cells.
BEAS-2B lung epithelial cell line and the MucilAirTM 3D lung model
Publications:
Rossner, P., Jr., Cervena, T., Vojtisek-Lom, M., Vrbova, K., Ambroz, A., Novakova, Z., Elzeinova, F., Margaryan, H., Beranek, V., Pechout, M., Macoun, D., Klema, J., Rossnerova, A., Ciganek, M., Topinka, J.: (2019) The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAirTM) and in Human Bronchial Epithelial Cells (BEAS-2B). International Journal of Molecular Sciences. 20(22). pii: E5710. doi: 10.3390/ijms20225710. IF=4.331
3. Molecular responses in THP-1 macrophage-like cells exposed to diverse nanoparticles
Nanomaterials (NM), as foreign structures, are recognized by immune cells, especially by macrophages. In this study, we compared the effects of diverse NM (TiO2, ZnO, SiO2, Ag) on THP-1 macrophage-like cells. While no significant effects were observed in cells exposed to TiO2, the other NM induced production of proinflammatory molecules, with Ag exhibiting also immunosupressive effects. The obtained results contribute to the understanding of the molecular mechanisms of NM toxicity, emphasing the role of immune cells and differences among various types of NM.
Scheme of the effects of nanomaterials on the immune function of macrophages
Publications:
Brzicova, T., Javorkova, E., Vrbova, K., Zajicova, A., Holan, V., Pinkas, D., Philimonenko, V., Sikorova, J., Klema, J., Topinka, J., Rossner, P., Jr.: (2019) Molecular Responses in THP-1 Macrophage-Like Cells Exposed to Diverse Nanoparticles. Nanomaterials (Basel). 9(5). pii: E687. doi: 10.3390/nano9050687. IF=3.811
Important results in 2018
1. Transcriptional response to organic compounds from diverse gasoline and biogasoline fuel emissions in human lung cells
We collected and characterized gasoline exhaust particles (GEPs) produced by Gasoline Direct Injection (GDI) engine running neat gasoline fuel (E0) and its blends with 15% ethanol (E15), 25% n-butanol (n- But25) and 25% isobutanol (i-But25). To study the toxic effects of organic compounds extracted from GEPs, we analyzed gene expression profiles in human lung BEAS-2B cells. Our results indicate that i-But25 extract possessed the weakest genotoxic potency possibly due to the low PAH content.
Scheme of the study evaluating the transcriptional response to organic compounds from four diverse gasoline and biogasoline fuel emissions in human lung epithelial cells BEAS-2B.
Publications:
Libalova, H., Rossner, P. Jr., Vrbova, K., Brzicova, T., Sikorova, J., Vojtisek-Lom, M., Beranek, V., Klema, J., Ciganek, M., Machala, M., Topinka, J.: (2018) Transcriptional response to organic compounds from diverse gasoline and biogasoline fuel emissions in human lung cells. Toxicology in Vitro. 48: 329-341. doi: 10.1016/j.tiv.2018.02.002. IF=3.067
2. Gene expression profiling in healthy newborns from diverse localities of the Czech Republic
We analyzed whole genome expression in cord blood leukocytes of 202 newborns from the Czech Republic, differing localities and levels of air pollution. We aimed to identify differentially expressed genes (DEGs) and pathways in relation to locality. The highest number of DEGs was found in samples from Karvina. A pathway analysis revealed a deregulation of processes associated with cell growth, apoptosis or cellular homeostasis, immune response-related processes or oxidative stress response.
Heatmap of the most differentially expressed genes
Publications:
Honkova, K., Rossnerova, A., Pavlikova, J., Svecova, V., Klema, J., Topinka, J., Milcova, A., Libalova, H., Choi, H., Veleminsky, M., Sram, R.J., Rossner, P. Jr.: (2018) Gene Expression Profiling in Healthy Newborns from Diverse Localities of the Czech Republic. Environmental and Molecular Mutagenesis.59: 401-415; doi: 10.1002/em.22184. IF=2.528
3. Inhalation of ZnO nanoparticles: splice junction expression and alternative splicing in mice
Biological effects of ZnO nanoparticles inhalation were investigated in mice. The inhalation affected splice junctions expression in genes participating in oxidative stress, apoptosis, immune response, inflammation and DNA repair. Further, alternative splicing in oxidative stress and inflammation-related genes was affected. In summary, ZnO nanoparticles have a potential negative effect on biological systems.
mRNA expression levels assessed by RT-qPCR for selected genes.
Publications:
Rössner, Jr.P., Vrbová, K., Strapáčová, S., Rössnerová, A., Ambrož, A., Brzicová, T., Libalová, H., Javorková, E., Kulich, P., Vecera, Z., Mikuska, P., Coufalik, P., Krumal, K., Capka, L., Docekal, B., Moravec, P., Sery, O., Misek, I., Fictum, P., Fiser, K., Machala, M., and Topinka, J.: (2018) Inhalation of ZnO nanoparticles: splice junction expression and alternative splicing in mice. Toxicological Sciences. 168(1): 190-200. doi: 10.1093/toxsci/kfy288. IF=3.654
Important results in 2017
1. Ultrafine and Fine Particles and Hospital Admissions in Central Europe. Results from the UFIREG Study
We investigated effects of ultrafine and fine particulate matter on cause-specific hospital admissions in Central and Eastern European cities. We found that a 2,750 particles/cm3 increase of ultrafine particles caused an increase in the risk of respiratory hospital admissions. We also found increases in the risk of cardiovascular and respiratory admissions associated with exposure to particulate matter of aerodynamic diameter < 2.5 µm.
Praticles size and their deposition in the respiratory tract ( http://www.ufireg-central.eu).
Publikace:
Lanzinger S, Schneider A, Breitner S, Stafoggia M, Erzen I, Dostal M, Pastorkova A, Bastian S, Cyrys J, Zscheppang A, Kolodnitska T, Peters A; UFIREG study group: (2016) Ultrafine and Fine Particles and Hospital Admissions in Central Europe. Results from the UFIREG Study. Am J Respir Crit Care Med.194(10):1233-1241. IF 13.204
2. Adaptation of the human population to the environment: Current knowledge, clues from Czech cytogenetic and "omics" biomonitoring studies and possible mechanisms
The main aim of this review was to provide a comprehensive overview of the last decade of Czech biomonitoring research, concerning the effect of various levels of air pollution and radiation, on the differently exposed population groups. A detailed analysis of data leads to a hypothesis of the versatile mechanism of adaptation to environmental stressors via DNA methylation settings which may even originate in prenatal development, and help to reduce the resulting DNA damage levels.
A model of epigenetic mechanism providing the link between changes of environmental factors during life and the levels of DNA damage.
Publikace:
Rössnerová, A., Pokorná, M., Švecová, V., Šrám, R., Topinka, J., Zölzer, F., Rössner, P.: (2017) Adaptation of the human population to the environment: Current knowledge, clues from Czech cytogenetic and "omics" biomonitoring studies and possible mechanisms. Mutation Research-Reviews in Mutation Research. 773: 188-203. IF=5.5. doi: 10.1016/j.mrrev.2017.07.002. Epub 2017 Jul 12. Review.
3. Blends of butanol and hydrotreated vegetable oils as drop-in replacement for diesel engines: Effects on combustion and emissions
We investigated the performance of a mix of two emerging biofuels, butanol and hydrotreated vegetable oil (HVO), in an Iveco Tector diesel engine. HVO, a possible drop-in fuel for diesel engines features excellent combustion and emissions characteristics. All biofuels exhibited, relative to diesel fuel, a decrease in the concentration of particulate PAHs and n-alkanes in emissions, while the concentrations of hopanes and steranes originated from lubricating oil were comparable across all fuels. Cold starts yielded about 15% higher concentrations of both particulate matter and particulate organic compounds in emissions than hot startsparticulate matter and particulate organic compounds in emissions than hot starts.
Sampling instrumentation Digitel for particulate engine emissions
Publikace:
Vojtíšek-Lom, M., Beránek, V., Mikuška, P., Křůmal, K., Coufalík, P., Sikorová, J., Topinka, J.: (2017) Blends of butanol and hydrotreated vegetable oils as drop-in replacement for diesel engines: Effects on combustion and emissions. Fuel, 197: 407-421. IF 4,601
Important results in 2016
1. Comparative Analysis of Toxic Responses of Organic Extracts from Diesel and Selected Alternative Fuels Engine Emissions in Human Lung BEAS-2B Cells
Exhaust particles from four different diesel/biodiesel fuels were collected, organic compounds were extracted and human lung cells were exposed to each extract. Chemical analysis revealed that extract from neat hydrotreated vegetable oil NexBTL contained the lowest content of carcinogenic PAHs and gene expression profiling identified deregulation of numerous genes and processes common for all extract as well as specific for each extract. Despite subtle differences in overall toxic effects induced by different diesel or alternative fuels, NexBTL extract exhibited the weakest toxicity comparing to others.
Sampling, organic extraction and toxicogenomic analysis of different diesel/alternative fuel exhaust particles.
Exhaust particles from standard diesel (B0), biodiesel (methylesters of rapeseed oil) in its neat form (B100) and 30% by volume blend with diesel fuel (B30), and neat hydrotreated vegetable oil (NEXBTL100) was collected and characterized. Global gene expression changes were analyzed in BEAS-2B cells following 4 h and 24 h treatment with orgaic compounds extracted from particles. The subtoxic concentrations of 50 µg extract/mL induced a similar molecular response. The common processes induced after 4 h treatment included antioxidant defense, metabolism of xenobiotics and lipids, suppression of pro-apoptotic stimuli, or induction of plasminogen activating cascade; 24 h treatment affected fewer processes, particularly those involved in detoxification of xenobiotics, including PAHs. The majority of distinctively deregulated genes detected after both 4 h and 24 h treatment were induced by NEXBTL100; the deregulated genes included, e.g., those involved in antioxidant defense and cell cycle regulation and proliferation.
Publication:
Helena Libalova, Pavel Rossner Jr., Kristyna Vrbova, Tana Brzicova, Jitka Sikorova, Michal Vojtisek-Lom, Vit Beranek, Jiri Klema, Miroslav Ciganek, Jiri Neca, Katerina Pencikova, Miroslav Machala, Jan Topinka:(2016) Comparative Analysis of Toxic Responses of Organic Extracts from Diesel and Selected Alternative Fuels Engine Emissions in Human Lung BEAS-2B Cells. International Journal of Molecular Sciences. IF 3.257
2. Urinary 8-oxo-7,8-dihydro-2‘-deoxyguanosine analysis by an improved ELISA: An inter-laboratory comparison study
We performed an inter-laboratory comparison of 8-oxo-7,8-dihydro-2‘-deoxyguanosine by ELISA using standardized conditions, compared the ELISA results with high-performance liquid chromatography-tandem mass spectrometry and performed identification of compounds that may contribute to the discrepancy between methods. Assay standardization significantly improved inter-laboratory agreement, although some variability caused by aromatic and heterocyclic compounds and saccharides is still observed.
An improved ELISA to assess urinary 8-oxo-7,8-dihydro-2‘-deoxyguanosine (8-oxodG)
Using standardized conditions urinary 8-oxodG levels were analyzed. Assay standardization significantly decreased inter-laboratory variability of the assay.
Publication:
Rössner, P., Orhan, H., Koppen, G., Sakai, K., Santella, R.M., Ambrož, A., Rossnerová, A., Šrám, R.,Ciganek, M., Neca, J., Arzuk, E., Mutlu, N., Cooke, M.S.: (2016) Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine analysis by an improved ELISA: An inter-laboratory comparison study. Free Radic. Biol. Med., 22;95: 169-179. IF 5.784
3. The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells
Cobalt-zinc-ferrite nanoparticles (NPs) encapsulated by amorphous silica were tested to assess their effect on DNA, lipids and proteins in rat mesenchymal stem cells. No significant cytotoxic effects were observed, except for the highest dose of CZF-NPs, that slowed down cell proliferation and induced damage to DNA, lipids and proteins. The results suggest that the silica-coated CZF-NPs, when applied at a non-toxic dose, represent a promising contrast agent for cell labeling for magnetic resonance analysis.
Publication:
Novotná, B., Turnovcová, K., Veverka, P., Rössner, P. Jr., Bagryantsevá, Y., Herynek, V., Zvatora, P., Vosmanská, M., Klementová, M., Syková, E., Jendelová, P.: (2016)The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells. Nanotoxicology, 10(6): 662-670. IF 7.913
Important results in 2015
1. Analysis of genetic damage in lymphocytes of former uranium processing workers
The frequency of cells containing micronuclei (MN) and the presence of centromeres in the MN were analyzed in lymphocytes of men from Southern Bohemia (former workers of a former uranium processing plant „MAPE Mydlovary“ and controls). No differences were found between formerly exposed workers and the control group. Moreover former workers with X-ray examination had significantly lower level of DNA damage than the control group. Possible reason of this results may be adaptation of the organism.
Examples of cytochalasin-B-blocked human peripheral blood lymphocytes stained with DAPI (blue) and Pan Centromeric probes with FITC (green): (a) Overall view (100x), (b) binucleated cell (BNC) with one centromere-positive (CEN+) micronucleus (MN) (1000x), (c) BNC with one centromere-negative (CEN-) MN (1000x) and (d) BNC with two MN, both CEN- (1000x).
Publication:
Zölzer, F., Havránková, R., Freitinger Skalická, Z., Rössnerová, A., Šrám, R.J.: (2015) Analysis of Genetic Damage in Lymphocytes of Former Uranium Processing Workers. Cytogenet Genome Res. 2015; 147 (1): 17-23
2. Day-to-day variability of toxic events induced by organic compounds bound to size segregated atmospheric aerosol
The temporal variability of size-segregated aerosol mass collected on a daily basis during the winter period in highly polluted districts of Ostrava city as well as toxic effects of organic compounds extracted from each size fraction were quantified. We revealed that upper accumulation mode fraction (0.5 < dae < 1 μm) comprises most of the aerosol mass and contained 44% of total c-PAHs while ultrafine fraction (< 0.17 μm) contained only 11% of c-PAHs. DNA adduct levels and dioxin-like activity strongly correlated with both aerosol mass and c-PAH concentrations suggesting genotoxicity as a major toxic effect of organic compounds bound to size-segregated aerosol.
(a)
Sum of c-PAH concentrations in four aerosol fractions per mg of the size fraction mass. c-PAHs include benz[a]anthracene, chrysene, benzo[k]fluoranthene, benzo[a]pyrene, dibenz[a,h]anthracene and indeno[1,2.3-cd]pyrene.
(b) Total DNA adduct levels induced by organic extracts from size-segregated aerosols in an acellular system (calf thymus DNA) per mg of the size fraction mass. A strong positive correlation was found between c-PAH content in different aerosol size fractions and DNA adducts (R: 0.62 – 0.86).
Publication:
Topinka, J., Rossner, P. Jr., Milcová, A., Schmuczerová, J., Pěnčíková, K., Rossnerová, A., Ambrož, A., Štolcpartová, J., Bendl, J., Hovorka, J., Machala, M.: (2015) Day-to-day variability of toxic events induced by organic compounds bound to size segregated atmospheric aerosol. Environ. Pollut. 202: 135-145.
3. Reduced gene expression levels after chronic exposure to high concenrations of air pollutants
We analyzed the effect of air pollution on gene expression in subjects living in Prague and in the polluted Ostrava region. We expected to observe changes in expression of DNA repair genes in subjects from the Ostrava region. Unexpectedly, the changes were found in Prague subjects, particularly in genes associated with immune response and neurodegenerative diseases. The results suggest that chronic exposure to air pollution may result in adaptation of the organism with possible negative health effects.
Pathways associated with air pollution in subjects from Prague and the Ostrava region. The figure shows both the pathways associated with location only and the pathways associated with exposure to air pollution (B[a]P – benzo[a]pyrene, PM2.5 – particulate matter of aerodynamic diameter < 2.5 µm).
Publication:
Rossner, Jr. P., Tulupová, E., Rossnerová, A., Líbalová, H., Hoňková, K., Gmuender, H., Pastorková, A., Švecová, V., Topinka, J., Šrám, R.J.: (2015) Reduced gene expression levels after chronic exposure to high concentrations of air pollutants. Mutat Res. 780:60-70.
2022
Hermanova B, Riedlova P, Dalecka A, Jirik V, Janout V, Sram RJ: (2022) Air pollution and molecular changes in age-related diseases. Int J Environ Health Res. 32(4):772-790. doi: 10.1080/09603123.2020.1797643. Epub 2020 Jul 29. PMID: 32723182
2021
Vojtisek-Lom, M .; Suta, M.; Sikorova, J.; Sram, R.J. (2021): High NO2 Concentrations Measured by Passive Samplers in Czech Cities: Unresolved Aftermath of Dieselgate? Atmosphere 2021, 12, 649. https://doi.org/10.3390/atmos12050649
Kroupa M, Rachakonda S, Vymetalkova V, Tomasova K, Liska V, Vodenkova S, Cumova A, Rossnerova A, Vodickova L, Hemminki K, Soucek P, Kumar R, Vodicka P. (2021): Telomere length in peripheral blood lymphocytes related to genetic variation in telomerase, prognosis and clinicopathological features in breast cancer patients. Mutagenesis. 2020 Dec 31;35(6):491-497. doi: 10.1093/mutage/geaa030. PMID: 33367858
2020
Jiraková, K., Moskvin, M., Machová Urdzíková, L., Rössner, P., Elzeinová, F., Chudíčková, M., Jirák, D., Ziolkowska, N., Horák, D., Kubinová, Š., Jendelová, P.: (2020) The negative effect of magnetic nanoparticles with ascorbic acid on peritoneal macrophages. Neurochem Res. 45(1): 159-170. Epub 2019. doi: 10.1007/s11064-019-02790-9. IF= 2.782
Blazkova, B., Pastorkova, A., Solansky, I., Veleminsky, Jr. M., Veleminsky, M., Urbancova, K., Vondraskova, V., Hajslova, J., Pulkrabova, J., Sram, R.J.: (2020) Effect of Polycyclic Aromatic Hydrocarbons Exposure on Cognitive Development in 5 Years Old Children. Brain Sci. 10(9): E619. doi: 10.3390/brainsci10090619. IF= 3.332
Blazkova, B., Pastorkova, A., Solansky, I., Veleminsky, M., Jr., Veleminsky, M., Rossnerova, A., Honkova, K., Rossner, Jr., P., Sram, R.J.: (2020) The Impact of Cesarean and Vaginal Delivery on Results of Psychological Cognitive Test in 5 Year Old Children. Medicina. 56(10): 554. doi: 10.3390/medicina56100554. IF= 1.205
Cejka, C., Kossl, J., Holan, V., Zhang, J.H., Cejkova, J.: (2020) An Immunohistochemical Study of the Increase in Antioxidant Capacity of Corneal Epithelial Cells by Molecular Hydrogen, Leading to the Suppression of Alkali-Induced Oxidative Stress. Oxid. Med. Cell. Longev. 7435260. doi: 10.1155/2020/7435260. IF= 5.076
Cervena, T., Vojtisek-Lom, M., Vrbova, K., Ambroz, A., Novakova, Z., Elzeinova, F., Sima, M., Beranek, V., Pechout, M., Macoun, D., Klema, J., Rossnerova, A., Ciganek, M., Topinka, J., Rossner, P. Jr.: (2020) Ordinary Gasoline Emissions Induce a Toxic Response in Bronchial Cells Grown at Air-Liquid Interface. Int. J Mol. Sci. 23;22(1):79. doi: 10.3390/ijms22010079. IF= 4.556
Novotna, B., Pelclova, D., Rossnerova, A., Zdimal, V., Ondracek, J., Lischkova, L., Vlckova, S., Fenclova, Z., Klusackova, P., Zavodna, T., Topinka, J., Komarc, M., Dvorackova, S., Rossner, P.: (2020) The Genotoxic Effects in the Leukocytes of Workers Handling Nanocomposite Materials. Mutagenesis. 12;35(4):331-340. doi:10.1093/mutage/geaa016. IF= 3.379
Polachova, A., Gramblicka, T., Parizek, O., Sram, R.J., Stupak, M., Hajslova, J., Pulkrabova, J.: (2020) Estimation of Human Exposure to Polycyclic Aromatic Hydrocarbons (Pahs) Based on the Dietary and Outdoor Atmospheric Monitoring in the Czech Republic. Environ. Res.. 182:108977. doi: 10.1016/j.envres.2019.108977. IF= 5.715
Prichystalova, R., Caron-Beaudoin, E., Richardson, l., Dirkx, E., Amadou, A., Zavodna, T., Cihak, R., Cogliano, V., Hynes J., Pelland-St-Pierre, L., Verner,M.A., van Tongeren, M., Ho, V.: (2020) An Approach to Classifying Occupational Exposures to Endocrine Disrupting Chemicals by Sex Hormone Function Using an Expert Judgment Process. J. Expo. Sci. Environ. Epidemiol. Jul 23. doi: 10.1038/s41370-020-0253-z. IF= 3.351
Rossner, P., Libalova, H., Vrbova, K., Cervena, T., Rossnerova, A., Elzeinova, F., Milcova, A., Novakova, Z., Topinka, J.: (2020) Genotoxicant Exposure, Activation of the Aryl Hydrocarbon Receptor, and Lipid Peroxidation in Cultured Human Alveolar Type II A549 Cells. Mutat. Res. 853:503173. doi: 10.1016/j.mrgentox.2020.503173. IF= 2.506
Rossnerova, A., Izzotti, A., Pulliero, A., Bast, A., Rattan, S. I. S., Rossner, P.: (2020) The Molecular Mechanisms of Adaptive Response Related to Environmental Stress. Intl. J. Mol. Sci. 21(19): E7053. doi: 10.3390/ijms21197053. IF= 4.556
Simkova, S., Veleminsky, M., Sram, J.R.: (2020) The Impact of Air Pollution to Obesity. Neuro. Endocrinol. Lett. 41(3): 101-108. IF= 0.750
Vondracek, J., Pencikova, K., Ciganek, M., Pivnicka, J., Karasova, M., Hyzalova, M., Strapacova, S., Palkova, L., Neča, L., Matthews, J., Vojtisek Lom, M., Topinka, J., Milcova, A., Machala, M.: (2020) Environmental Six-Ring Polycyclic Aromatic Hydrocarbons Are Potent Inducers of the Ahr-Dependent Signaling in Human Cells.Environ. Pollut. 266(Pt 2): 115125. doi: 10.1016/j.envpol.2020.115125. IF= 6.792
Xu, J., Sram, R.J., Cebulska-Wasilewska, A., Miloradov, M.V., Sardas, S., Au, W.W.: (2020) Challenge-Comet Assay, a Functional and Genomic Biomarker for Precision Risk Assessment and Disease Prevention Among Exposed Workers. Toxicol. Appl. Pharmacol. 397:115011. 10.1016/j.taap.2020.115011. IF= 3.347
2019
Brzicova, T., Sikorova, J., Milcova, A., Vrbova, K., Klema, J. , Pikal, P., Lubovska, Z., Philimonenko, A., Franco, F., Topinka, J., Rossner, P., Jr.: (2019) Nano-TiO2 stability in medium and size as important factors of toxicity in macrophage-like cells. Toxicology in Vitro. 54: 178-188. doi: 10.1016/j.tiv.2018.09.019. IF= 3.067
Cervena, T., Vrbova, K., Rossnerova, A., Topinka, J., Rossner, P., Jr. : (2019) Short-term and Long-term Exposure of the MucilAir™ Model to Polycyclic Aromatic Hydrocarbons. Altern Lab Anim. 47(1): 9-18. doi: 10.1177/0261192919841484. IF= 3.014
Choi, H., Song, WM., Wang, M., Dostal, M., Pastorkova, A., Libalova, H., Tulupova, E., Rossnerova, A., Rossner, P. Jr., Sram, RJ., Zhang, B. (2019): Benzo[a]pyrene is associated with dysregulated myelo-lymphoid hematopoiesis in asthmatic children. Environ Int. 128: 218-232. IF= 7.943
Holan, V., Javorkova, E., Vrbova, K., Vecera, Z., Mikuska, P., Coufalik, P., Kulich, P., Skoupy, R., Machala, M., Zajicova, A., Rossner, P.: (2019) A murine model of the effects of inhaled CuO nanoparticles on cells of innate and adaptive immunity – a kinetic study of a continuous three-month exposure. Nanotoxicology.13(7): 952-963. doi: 10.1080/17435390.2019.1602679. IF= 5.811
Novotná, B ., Sikorová, J., Milcová, A., Pechout, M., Dittrich, L., Vojtíšek-Lom, M, Rössner, P., Brzicová, T., Topinka, J.: (2019) The genotoxicity of organic extracts from particulate truck emissions produced at various engine operating modes using diesel or biodiesel (B100) fuel: A pilot study. Mutation Research – Genetic Toxicology. 845: 403034. doi: 10.1016/j.mrgentox.2019.03.007. IF= 2.256
Rossner, P., Libalova, H., Cervena, T., Vrbova, K., Elzeinova, F., Milcova, A., Rossnerova, A., Novakova, Z., Ciganek, M., Pokorna, M., Ambroz, A., Topinka, J.: (2019) The processes associated with lipid peroxidation in human embryonic lung fibroblasts, treated with polycyclic aromatic hydrocarbons and organic extract from particulate matter. Mutagenesis. 34: 153-164. pii: gez004. doi: 10.1093/mutage/gez004. IF= 2.638
Rossnerova, A ., Pelclova, D., Zdimal, V., Rossner, P, Elzeinova, F., Vrbova, K., Topinka, J., Schwarz, J., Ondracek, J., Kostejn, M., Komarc, M., Vlckova, S., Fenclova, Z., Dvorackova, S.: (2019) The repeated cytogenetic analysis of subjects occupationally exposed to nanoparticles: a pilot study. Mutagenesis. 34(3): 253-363. IF= 2.898
Svobodová, J., Procházková, J., Kabátková, M., Krkoška, M., Šmerdová, L., Líbalová, H., Topinka, J., Kléma, J., Kozubík, A., Machala, M., Vondráček, J.: (2019) 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) disrupts control of cell proliferation and apoptosis in a human model of adult liver progenitors. Toxicological Sciences. 172(2): 368-384. doi: 10.1093/toxsci/kfz202. IF= 3.564
Tylichová, Z., Neča, J., Topinka, J., Milcová, A., Hofmanová, J., Kozubik, A., Machala, M., Vondráček, J.: (2019) n-3 Polyunsaturated fatty acids alter benzo[a]pyrene metabolism and genotoxicity in human colon epithelial cell models. Food and Chemical Toxicology. 124: 374-384. IF= 3.775
2018
Procházková, J., Střapacova, S., Svrzkova, L., Andrysík, Z., Hýžd'alová, M., Hrubá, E., Pěnčíková, K., Líbalová, H., Topinka, J., Kléma, J., Espinosa, J. M., Vondráček, J., Machala, M.: (2018) Adaptive changes in global gene expression profile of lung carcinoma A549 cells acutely exposed to distinct types of AhR ligands. Toxicology Letters. 292: 162-174. doi: 10.1016/j.toxlet.2018.04.024. IF=3.749
Choi, H., Dostál, M., Pastorková, A., Rössner, P., Šrám, R., Ho, S.M.: (2018) Greater susceptibility of girls to airborne Benzo[a]pyrene for obesity-associated childhood asthma. Environment International. 121: 308-316. doi: 10.1016/j.envint.2018.08.061. IF=8.763
Hoňková, K., Rössnerová, A., Pavlíková, J., Švecová, V., Kléma, J.,Topinka, J., Milcová, A., Líbalová, H. , Choi, H., Veleminský Jr., M., Šrám, R., Rössner ml., P.: (2018) Gene expression profiling in healthy newborns from diverse localities of the Czech Republic. Environmental and Molecular Mutagenesis. 59 (5): 401-415. doi: 10.1002/em.22184. IF=2.528
Leoni, C., Pokorná, P., Hovorka, J., Masiol, M., Topinka, J., Zhao, Y., Křůmal, K., Cliff, S., Mikuška, P., Hopke, P.K.: (2018) Source apportionment of aerosol particles at a European air pollution hot spot using particle number size distributions and chemical composition. Environmental Pollution. 234: 145-154. doi: 10.1016/j.envpol.2017.10.097. IF=5.099
Libalova, H ., Costa, P.M., Olsson M., Farcal, L., Ortelli, S., Blosi, M., Topinka, J., Costa, A.L., Fadeel, B.: (2018) Toxicity of surface-modified copper oxide nanoparticles in a mouse macrophage cell line: Interplay of particles, surface coating and particle dissolution. Chemosphere. 196: 482-493. doi: 10.1016/j.chemosphere.2017.12.182. IF=4.208
Libalova, H., Milcova, A., Cervena, T., Vrbova, K., Rossnerova, A., Novakova, Z., Topinka, J., Rossner, P., Jr. : (2018) Kinetics of ROS generation induced by polycyclic aromatic hydrocarbons and organic extracts from ambient air particulate matter in model human lung cell lines. Mutat Res Gen Tox En. 827:50-58. doi: 10.1016/j.mrgentox.2018.01.006. IF=2.133
2017
Broday, D. M., Arpaci, A., Bartoňová, A., Castell-Balaguer, N., Cole-Hunter, T., Dauge, F.R., Fishbain, B., Jones, R.L., Galea, K., Jovasevic-Stojanovic, M., Kocman, D., Martinez-Iniguez, T., Nieuwenhuijsen, M., Robinson, J., Švecová, V., Thai, P.: (2017) Wireless distributed environmental sensor networks for air pollution measurement-the promise and the current reality. Sensors. 17 (10): 2263. IF= 3.032
Červená, T., Rössnerová, A., Sikorová, J. , Beránek, V., Vojtíšek-Lom, M., Ciganek, M., Topinka, J., Rössner, P. Jr.: (2017) DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells. Basic & Clinical Pharmacology & Toxicology. 121 (SI): 102-108. IF=2.452
Choi, H., Tabashidze, N., Rössner, P., Dostál, M., Pastorková, A., Kong, S.W., Gmuender, H., Šrám, R.: (2017) Altered vulnerability to asthma at various levels of ambient Benzo[a]Pyrene by CTLA4, STAT4 and CYP2E1 polymorphisms. Environmental Pollution. 231: 1134-1144. IF= 6.152
Rössnerová, A., Pokorná, M., Švecová, V., Šrám, R., Topinka, J. , Zölzer, F., Rössner, P.: (2017) Adaptation of the human population to the environment: Current knowledge, clues from Czech cytogenetic and "omics" biomonitoring studies and possible mechanisms. Mutation Research-Reviews in Mutation Research. 773: 188-203. IF= 6.633
Urbancová, K., Lanková, D., Rössner ml., P., Rössnerová, A., Švecová, V., Tomaniová, M., Veleminský Jr., M., Šrám, R., Hajslová, J., Pulkrabová, J.: (2017) Evaluation of 11 polycyclic aromatic hydrocarbon metabolites in urine of Czech mothers and newborns. Science of the Total Environment. 577: 212-219. IF= 5.727
Veleminský, M., Hanzl, M., Šrám, R.: (2017) The impact of air pollution in the Southern Bohemia Region on fetuses and newborns. Neuroendocrinology Letters. 37(2): 52-57. IF=0.918
Vojtíšek-Lom, M., Beránek, V., Mikuška, P., Křůmal, K., Coufalík, P., Sikorová, J., Topinka, J.: (2017) Blends of butanol and hydrotreated vegetable oils as drop-in replacement for diesel engines: Effects on combustion and emissions. Fuel. 197: 407-421. IF=5.223
Zapletal, O, Tylichová, Z., Neča, J., Kohoutek, J., Machala, M., Milcová, A., Pokorná, M., Topinka, J., Moyer, M.P., Hofmanová, J., Kozubík, A., Vondráček, J.: (2017) Butyrate alters expression of cytochrome P450 1A1 and metabolism of benzo[a]pyrene via its histone deacetylase activity in colon epithelial cell models. Archives of Toxicology. 91(5): 2135-2150. IF=6.044
Zölzer, F., Křížová, M., Skalická, Z.F., Rössnerová, A., Šrám, R.: (2017) Micronucleus frequency and content in healthy relatives of cancer patients. Biomarkers. 22(7): 667-673. IF=1.976
Čejka, Č., Kössl, J., Heřmánková, B., Holáň, V., Čejková, J.: (2017) Molecular hydrogen effectively heals alkali-injured cornea via suppression of oxidative stress. Oxidative Medicine and Cellular Longevity. 8906027. IF=4.936
2016
Ambrož, A., Vlková, V., Rössner, P. Jr., Rössnerová, A., Švecová, V., Milcová, A. , Pulkrabová, J., Hajslová, J., Veleminský, M. Jr., Solanský, I., Šrám, R.: (2016) Impact of air pollution on oxidative DNA damage and lipid peroxidation in mothers and their newborns. Int. J. Hyg. Environ. Health., 219 (6): 545-556.
Andersen, Z. J., Šrám, R. J., Ščasný, M., Gurzau, E. S., Fucic, A., Gribaldo, L., Rossner, P. Jr., Rossnerová, A., Braun Kohlová, M., Máca, V., Zvěřinová, I., Gajdosová, D., Moshammer, H., Rudnai, P., Knudsen, L. E.: (2016) Newborns health in the Danube Region: Environment, biomonitoring, interventions and economic benefits in a large prospective birth cohort study. Environ. Int., 88: 112-122.
Šrám, R.J., Rössner, P., Rössnerová, A., Dostál, M., Milcová, A., Švecová, V. , Pulkrabová, J., Hajšlová, J., Velemínský, M.: (2016) Impact of Air Pollution to Genome of Newborns. Am. J. Respir. Crit. Care Med., 24: S40-S44. IF=15.196
Veleminský, M., Ambrož, A., Rössner, P., Rössnerová, A., Švecová, V., Milcová, A., Dostál, M., Pastorková, A., Pulkrabová, J., Hajslová, J., Solanský, I., Hanzl, M., Šrám, R.: (2016) Oxidative stress in newborns by different modes of delivery. Neuroendocrinology Letters. 37(6): 445-451. IF=0.918
Lanzinger, S., Schneider A., Breitner, S., Stafoggia, M., Erzen, I., Dostál, M., Pastorková, A., Bastian, S., Cyrys, J., Zscheppang. A., Kolodnitska, T., Peters, A.: (2016) Ultrafine and Fine Particles and Hospital Admissions in Central Europe. Results from the UFIREG Study. American Journal of Respiratory and Critical Care Medicine. 194(10): 1233-1241. IF= 15.196
Fucic, A., Bonassi, S., Gundy, S., Lazutka, J., Šrám, R., Ceppi, M., Lucas, J.N.: (2016) Frequency of Acentric Fragments Are Associated with Cancer Risk in Subjects Exposed to Ionizing Radiation. Anticancer Res., 36(5): 2451-2457.
Fishbain, B., Lerner, U., Castell, N., Cole-Hunter, T., Popoola, O., Broday, D.M., Iñiguez, T.M., Nieuwenhuijsen, M., Jovasevic-Stojanovic, M., Topalovic, D., Jones, R.L., Galea. K.S., Etzion, Y., Kizel, F., Golumbic, Y.N., Baram-Tsabari, A., Yacobi, T., Drahler, D., Robinson, J.A., Kocman, D., Horvát, M., Švecová, V., Arpaci, A., Bartonová, A.: (2016) An evaluation tool kit of air quality micro-sensing units. Science of the Total Environment. 575: 639-648. IF= 5.727
Ghosh, R., Rossner, P., Hoňková, K., Dostál, M., Šrám, R., Hertz-Picciotto, I.: (2016) Air pollution and childhood bronchitis: Interaction with xenobiotic, immune regulatory and DNA repair genes. Environ. Int., 87: 94-100.
Lanková, D., Urbancová, K., Šrám, R., Hajslová, J., Pulkrabová, J.: (2016) A novel strategy for the determination of polycyclic aromatic hydrocarbon monohydroxylated metabolites in urine using ultra-high-performance liquid chromatography with tandem mass spectrometry. Anal. Bioanal. Chem., 408(10): 2515-2525.
Lanzinger, S., Schneider, A., Breitner, S., Stafoggia, M., Erzen, I., Dostál, M., Pastorková, A., Bastian, S., Cyrys, J., Zscheppang, A., Kolodnitská, T., Peters, A.: (2016) Associations between ultrafine and fine particles and mortality in five central European cities - Results from the UFIREG study. Environ. Int., 88: 44-52.
Novotná, B., Turnovcová, K. , Veverka, P., Rössner, P. Jr., Bagryantsevá, Y., Herynek, V., Zvatora, P., Vosmanská, M., Klementová, M., Syková, E., Jendelová, P.: (2016) The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells. Nanotoxicology, 10(6): 662-670.
Pulkrabová, J., Stupak, M., Švarcová, A., Rössner, P., Rössnerová, A., Ambrož, A., Šrám, R., Hajslová, J.: (2016) Relationship between atmospheric pollution in the residential area and concentrations of polycyclic aromatic hydrocarbons (PAHs) in human breast milk. Sci. Total Environ., 562: 640-647.
Rössner, P., Orhan, H., Koppen, G., Sakai, K., Santella, R.M., Ambrož, A., Rossnerová, A., Šrám, R., Ciganek, M., Neca, J., Arzuk, E., Mutlu, N., Cooke, M.S.: (2016) Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine analysis by an improved ELISA: An inter-laboratory comparison study. Free Radic. Biol. Med., 22;95: 169-179.
Rössnerová, A., Hoňková,K., Pavlíková, J. , Freitinger-Skalická, Z., Havránková,R., Solanský, I., Rössner, P. Jr., Šrám, R.J., Zölzer, F.: (2016) Mapping the factors affecting the frequency and types of micronuclei in an elderly population from Southern Bohemia. Mutat. Res. -Fundam. Mol. Mech. Mutagen., 793 - 794: 32-40.
Šrám, R.J., Švecová, V., Rössnerová, A.:
(2016) Systematic review of the use of the lymphocyte cytokinesis-block micronucleus assay to measure DNA damage induced by exposure to polycyclic aromatic hydrocarbons. Mutat. Res. -Fundam. Mol. Mech. Mutagen., 770(Pt A):162-169.
Urbancová, K., Lanková, D., Rössner, P., Rössnerová, A., Švecová, V., Tomaniová, M., Veleminský, M. Jr., Šrám, R.J., Hajslová, J., Pulkrabová, J.: (2016) Evaluation of 11 polycyclic aromatic hydrocarbonmetabolites in urine of Czech mothers and newborns. Sci. Total Environ., 212-219.
Čejka, Č., Čejková, J., Trošan, P., Zajícová, A., Syková, E., Holáň, V.: (2016) Transfer of mesenchymal stem cells and cyclosporine A on alkali-injured rabbit cornea using nanofiber scaffolds strongly reduces corneal neovascularization and scar formation. Histology and Histopathology, 31: 969-980.
Čejková, J., Čejka, Č., Trošan, P., Zajícová, A., Syková, E., Holáň, V.: (2016) Treatment of alkali-injured cornea by cyclosporine A-loaded electrospun nanofibers – An alternative mode of therapy. Experimental Eye Research, 147: 128-137.
Fucic, A., Bonassi, S., Gundy, S., Lazutka, J., Šrám, R., Ceppi, M., Lucas, J.N.: (2016) Frequency of Acentric Fragments Are Associated with Cancer Risk in Subjects Exposed to Ionizing Radiation. Anticancer Res., 36(5): 2451-2457.
Andersen, Z. J., Šrám, R. J., Ščasný, M., Gurzau, E. S., Fucic, A., Gribaldo, L., Rossner, P. Jr., Rossnerová, A., Braun Kohlová, M., Máca, V., Zvěřinová, I., Gajdosová, D., Moshammer, H., Rudnai, P., Knudsen, L. E.: (2016) Newborns health in the Danube Region: Environment, biomonitoring, interventions and economic benefits in a large prospective birth cohort study. Environ. Int., 88: 112-122.
Lanková, D., Urbancová, K., Šrám, R., Hajslová, J., Pulkrabová, J.: (2016) A novel strategy for the determination of polycyclic aromatic hydrocarbon monohydroxylated metabolites in urine using ultra-high-performance liquid chromatography with tandem mass spectrometry. Anal. Bioanal. Chem., 408(10): 2515-2525.
2015
Zölzer, F., Havránková, R., Freitinger Skalická, Z., Rössnerová, A., Šrám, R.J.: (2015) Analysis of Genetic Damage in Lymphocytes of Former Uranium Processing Workers. Cytogenet. Genome Res.
2014
Cejkova, J., Olmiere, C., Cejka, C., Trosan, P., Holan, V.: (2014) The healing of alkali-injured cornea is stimulated by a novel matrix regenerating agent (RGTA, CACICOL20) – a biopolymer mimicking heparan sulfates reducing proteolytic, oxidative and nitrosative damage. Histol. Histopathol. 29, 457-478.
Líbalová, H., Krčková, S., Uhlířová, K., Milcová, A., Schmuczerová, J., Ciganek, M., Kléma, J., Machala, M., Šrám, R., Topinka, J.: (2014) Genotoxicity but not the AhR-mediated activity of PAHs is inhibited by other components of complex mixtures of ambient air pollutants. Toxicol Lett. 225(3): 350-357.
Líbalová H., Krčková, S., Uhlířová, K., Klema, J., Ciganek, M., Rössner, P. Jr., Šrám, R., Vondráček, J., Machala, M., Topinka, J.: (2014) Analysis of gene expression changes in A549 cells induced by organic compounds from respirable air particles. Mutat. Res. -Fundam. Mol. Mech. Mutagen. 770: 94-105.
Průcha, M., Zazula, R., Herold, I., Dostál, M., Hyanek, T., Bellingan, G.: (2014) Presence of hypogammaglobulinemia in patients with severe sepsis, septic shock, and SIRS is associated with increased mortality. J. Infect. 68(3): 297-299.
Rössner, P., Jr, Rössnerová, A., Beskid, O., Tabashidze, N., Líbalová, H., Uhlířová, K., Topinka, J., Šrám, R.: (2014) Nonhomologous DNA end joining and chromosome aberrations in human embryonic lung fibroblasts treated with environmental pollutants. Mutat Res. (763-764): 28-38.
Zablotskii, V., Lunov, O., Novotná, B., Churpita, O., Trošan, P., Holáň, V., Syková, E., Dejneka, A., Kubinová, Š.: (2014) Down-regulation of adipogenesis of mesenchymal stem cells by oscillating high-gradient magnetic fields and mechanical vibration. Appl. Phys. Lett. 105(10): 103702
2013
Cejkova, J., Tosan, P., Cejka, C., Lencova, A., Zajicova, A., Javorkova, E., Kubinová, S., Sykova, E., Holan, V.: (2013) Suppression of alkali-induced oxidative injury to the cornea by mesenchymal stem cells growing on nanofiber scaffolds and transferred onto the damaged corneal surface. Exp. Eye Res. 116, 312-323.
Fenech, M., Kirsch-Volders, M., Rössnerová, A., Šrám, R., Romm, H., Bolognes,i C., Ramakumar, A., Soussaline, F., Schunck, C., Elhajouji, A., Anwar, W., Bonassi, S.: (2013) HUMN project initiative and review of validation, quality control and prospects for further development of automated micronucleus assays using image cytometry systemsInt. J. Hyg. Environ. Health. 216(5): 541-552.
Ghosh, R., Topinka, J., Joad, J. P., Dostál, M., Šrám R., Hertz-Picciotto, I.: (2013) Air pollutants, genes and early childhood acute bronchitis. Mutat. Res. -Fundam. Mol. Mech. Mutagen. 749(1-2): 80-86.
Hyánková, L., Novotná, B.: (2013) Divergent selection for shape of growth curve in Japanese quail: 7. Effect of egg storage at high temperature on embryo development and hatchability. Br. Poult. Sci. 54(6): 695-703.
Larsen, P. S., Kamper-Jørgensen, M., Adamson, A., Barros, H., Bonde, J. P., Brescianini, S., Brophy, S., Casas, M., Devereux, G., Eggesbø, M., Fantini, M. P., Frey, U., Gehring, U., Grazuleviciene, R., Henriksen, T. B., Hertz-Picciotto, I., Heude, B., Hryhorczuk, D. O., Inskip, H., Jaddoe, V. W., Lawlor, D. A., Ludvigsson, J., Kelleher, C., Kiess, W., Koletzko, B., Kuehni, C. E., Kull, I., Kyhl, H. B., Magnus, P., Momas, I., Murray, D., Pekkanen, J., Polanska, K., Porta, D., Poulsen, G., Richiardi, L., Roeleveld, N., Skovgaard, A. M.,Šrám, R., Strandberg-Larsen, K., Thijs, C., Van Eijsden, M., Wright, J., Vrijheid, M., Andersen, A.M.: (2013) Pregnancy and birth cohort resources in europe: a large opportunity for aetiological child health research. Paediatr. Perinat. Epidemiol. 27(4): 393-414.
Pauk, N., Klimešová, S., Kara, J., Topinka, J., Labaj, J.: (2013) The relevance of monitoring of antibodies against the polycyclic aromatic hydrocarbon (PAH) and PAH-DNA adducts in serum in relation to lung cancer and chronic obstructive pulmonary disease (COPD). Neoplasma 60(2): 182-187.
Rössner, P. Jr., Mistry, V., Singh, R., Šrám, R., Cooke M. S.: (2013) Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS. Biochem. Biophys. Res. Commun. 440(4): 725-730.
Rössner, P. Jr., Švecová, V., Schmuczerová, J., Milcová, A., Tabashidze, N., Topinka, J., Pastorková, A., Šrám, R.: (2013) Analysis of biomarkers in a Czech population exposed to heavy air pollution. Part I. Bulky DNA adducts. Mutagenesis. 28(1): 89-95.
Rössner, P. Jr., Rössnerová, A., Špátová, M., Beskid, O., Uhlířová, K., Líbalová, H., Solanský, I., Topinka, J., Šrám, R.: (2013) Analysis of biomarkers in a Czech population exposed to heavy air pollution. Part II. Chromosomal aberrations and oxidative stress. Mutagenesis. 28(1): 97-106.
Rössnerová, A., Tulupová, E., Tabashidze, N., Schmuczerová, J., Dostál, M., Rössner, P. Jr., Gmuender, H., Šrám, R.: (2013) Factors affecting the 27K DNA methylation pattern in asthmatic and healthy children from locations with various environments.Mutat. Res. -Fundam. Mol. Mech. Mutagen. 741-742: 18-26.
Slama, R., Bottagisi, S., Solanský, I., Lepeule, J., Giorgis-Allemans, L., Šrám, R.: (2013) Short-term impact of atmospheric pollution on fecundability. Epidemiology. 24(6): 871-879.
Šmerdová, L., Neca, J., Svobodová, J., Topinka, J., Schmuczerová, J., Kozubík, A., Machala, M., Vondrácek, J.: (2013) Inflammatory mediators accelerate metabolism of benzo[a]pyrene in rat alveolar type II cells: the role of enhanced cytochrome P450 1B1 expression. Toxicology. 314: 30-38.
Šrám, R., Binková, B., Dostál, M., Merkerová-Dostalová, M., Líbalová, H., Milcová, A., Rössner, P. Jr., Rössnerová, A., Schmuczerová, J., Švecová, V., Topinka, J., Votavová, H.: (2013) Health impact of air pollution to children. Int. J. Hyg. Environ. Health. 216(5): 533-540.
Švecová, V., Topinka, J., Solanský, I., Rössner, P. Jr., Šrám, R.: (2013) Personal exposure to carcinogenic polycyclic aromatic hydrocarbons in the Czech Republic. J. Expos. Sci. Environ. Epidemiol. 23(4): 350-355.
Topinka, J., Milcová, A., Schmuczerová, J., Krouzek, J., Hovorka, J.: (2013) Ultrafine particles are not major carriers of carcinogenic PAHs and their genotoxicity in size-segregated aerosols. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 754(1-2): 1-6.
Yauk, C. L., Bishop, J., Dearfield, K. L., Douglas, G. R., Hales, B. F., Luijten, M., O´Brien, J. M., Robaire, B., Šrám, R., van Bethem, J., Wade, M. G., White, P. A. Marchetti, F.: (2013) The development of adverse outcome pathways for mutagenic effects for the organization for economic co-operation and development. Environ. Mol. Mutagen. 54(2): 79-81.
2020–2022 EATRIS-CZ II Czech National Node of European Infrastructure in Translational Medicine (Ministry of Education, Youth and Sports, LM2018133)
2020–2022 ADAIR From air pollution to brain pollution – novel biomarkers to unravel the link of air pollution and Alzheimer’s disease
2020–2022 ProNanoEnviCz II Modernization VVI Nanomaterials and Nanotechnologies for Environmental Protection and Sustainable Future (Ministry of Education, Youth and Sports)
2020–2022 NanoEnviCz Research Infrastructure (Ministry of Education, Youth and Sports, LM2018124)
2019–2023 TUBE Transport derived Ultrafines and the Brain Effects (Horizon 2020, 814978-2)
2019–2023 COST action HARMONY – „Novel tools for test evaluation and disease prevalence estimation, https://www.cost.eu/actions/CA18208/#tabs|Name:overview
2018–2022 HAIE Healthy Aging in Industrial Environment (European Union – European Structural and Investment Funds, Operational Program Research, Development and Education; Ministry of Education, Youth and Sports, CZ.02.1.01/0.0/0.0/16_019/0000798)
2018–2021 GASODAM Cellular Response Mechanisms of Organic Matter Genotoxicity in Petrol and Alternative Fuel Emissions on the Human Pulmonary Cell Model (Czech Science Foundation, 18-06548Y)
2018–2020 MUCILTOX Mechanisms of toxicity of emissions from petrol engines in 3D tissue cultures and in model bronchial epithelial cell line (Czech Science Foundation, 18-04719S)
2018–2020 NANOEX The effect of nanoparticle exposure on DNA changes in the human population (Czech Science Foundation, 18-02079S)
2016–2021 Strategie AV21-QUALITAS research programme „Wellbeing in health and disease“
2016–2020 COST Action hCOMET The comet assay as a human biomonitoring tool (Cost Action CA 15132 hCOMET)
2015–2020 NANOGEN Genomics and Proteomics in the Study of Mechanisms of Biological Effects of Manufactured Nanoparticles (Ministry of Education, Youth and Sports, LO1508)
2015–2020 Nanoparticle Genotoxicity Measurement System (OPPK – Operational Program Prague Competitiveness, European Fund for Regional Development Prague & EU We invite you to your future, CZ.2.16/3.1.00/24507)
The National Institute of Occupational Health, Norway
Norwegian Institute for Air Research, Norway
Karolinska Institutet, Sweden
Institute of Occupational Medicine, Scotland
University of Manchester, UK
Flemish Institute for Technological Research, Belgium
University of Oslo, Norway
King’s College London, UK
Florida International University, MI, USA
University of Eastern Finland
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