Institute of Experimental Medicine CAS

Research topics:

Our research is focused on stem cells and biomaterials for tissue reconstruction or regeneration and evaluation their performance using biological models. In collaboration with the Institute of Physics AS CR, research of the effects of low-temperature atmospheric plasmas on biological systems as well as the development of novel devices for medical applications are performed.

Research projects:

  1. Study of therapeutic potential of mesenchymal stem cells from different sources (bone marrow, adipose tissue, umbilical cord) and their secretomes in vitro and in the models in vivo.
  2. Development of technology of stem cell cultivation and cryopreservation for clinical use.
  3. Development of hydrogels based on extracellular matrix and evaluation of their therapeutic potential in the regeneration of damaged nerve tissue.
  4. Development and study of low-temperature atmospheric pressure plasmas for biomedical applications. Preclinical and clinical testing of low-temperature plasmas in wound healing.
  5. Controlling stem cell fate and targeting stem cell delivery with high-gradient magnetic fields.

Deputy Head:

Irena Vacková, MSc, PhD.
E-mail: irena.vackova@biomed.cas.cz
Phone: +420 241 062 635

Research Scientists:

Assoc. Prof. Jitka Čejková, MD, DSc.
Čestmír Čejka, PhD.
Milada Chudíčková, PhD.
Šárka Kubinová, PharmD, PhD.
Yuri Petrenko, MSc, PhD.
Irena Vacková, MSc, PhD.

PhD. Students:

Jana Dubišová, MSc
Klára Hahnová, MSc
Noelia Martinez Varea, MSc
Karel Výborný, MSc
Kristýna Závišková, MSc

Technicans:

Linda Fedorowiczová
Jana Herlová
Lucie Svobodová, PhD.
Lenka Uherková, MSc, PhD.

Important results in 2017

1. Non-thermal air plasma promotes the healing of acute skin wounds in rats

Non-thermal plasma (NTP) has nonspecific antibacterial effects, and can be applied as an effective tool for the treatment of infected wounds and other skin pathologies. In this study we analysed the effect of NTP on the healing of the full-thickness acute skin wound model in rats. NTP treatment had improved the healing efficacy of acute skin wounds without noticeable side effects and concomitant activation of pro-inflammatory signalling. The obtained results highlight the favourability of plasma applications for wound therapy in clinics.

Publication: Kubinova S, Zaviskova K, Uherkova L, Zablotskii V, Churpita O, Lunov O, Dejneka A. Non-thermal air plasma promotes the healing of acute skin wounds in rats. Sci Rep. 2017; 7:45183.

 

  potkan-ntp

Healing of acute skin wound in rat after the treatment with low-temperature plasma.
(A) Experimental scheme. (B) Wound contraction in time intervals 1-14 days. Plasma treatment resulted in significant improvement of wound healing 7 days after the injury. 

 
2. Molecular hydrogen effectively heals the alkali-burned cornea via suppression of oxidative stress, decreases the inflammation in the anterior eye segment and prevents corneal neovascularization

The alkali-burn of the anterior eye segment evokes oxidative stress. The antioxidant mechanisms are exhausted leading to the antioxidant/prooxidant imbalance which is immediately followed by the protease/antiprotease imbalance. This situation results in the excessive inflammation. When the molecular hydrogen is dropped on the anterior eye surface early after the injury, it suppresses oxidative stress and thus decreases the inflammation and promotes the healing process.

Publication:  Čejka, Č., Kössl, J., Heřmánková, B., Holáň, V., Čejková, J.: (2017) Molecular Hydrogen Effectively Heals Alkali-Injured Cornea via Suppression of Oxidative Stress. Oxidative Medicine and Cellular Longevity. 8906027.

    cejk

The healing of the rabbit eye after alkali burn after the treatment with buffer or buffer with H2. The cornea healed with H2 is transparent with no sign of neovascularization (day 20). The treatment with H2 significantly decreased expression of vascular endothelial growth factor, which is characteristic for neovascularization.

 

Important result in 2016

Injectable extracellular matrix hydrogels as scaffolds for spinal cord injury repair.

An important strategy in spinal cord injury (SCI) repair is bridging the lesion with a supportive and stimulatory biomaterial that would support axonal rewiring. In this study, we prepared extracellular matrix (ECM) hydrogels by decellularization of porcine spinal cord or porcine urinary bladder and evaluated their neuroregenerative properties in vivo in the acute model of SCI. The results showed that both ECM hydrogels bridged the lesion cavity, modulated the innate immune response, and provided the benefit of a stimulatory substrate for in vivo neural tissue regeneration. ECM hydrogels thus represent a promising materials for the neural tissue engineering.

Publication: Tukmachev D, Forostyak S, Koci Z, Zaviskova K, Vackova I, Vyborny K, Sandvig I, Sandvig A, Medberry CJ, Badylak SF, Sykova E, Kubinova S. Injectable extracellular matrix hydrogels as scaffolds for spinal cord injury repair. Tissue Eng A. 2016; 22(3-4):306-17. IF 3.892

ecm-hydrogel

Scheme of preparation of hydrogels by decelularization of tissues and their application to spinal cord model.

 

Important result in 2015

An effective strategy of magnetic stem cell delivery for spinal cord injury therapy

In this study, we designed a magnetic system and used it to accumulate stem cells labelled with superparamagnetic iron oxide nanoparticles (SPION) at a specific site of a spinal cord injury lesion. Histological analysis of cell distribution on the spinal cord surface showed a good correlation with the calculated distribution of magnetic forces exerted onto the transplanted cells. The results suggest that focused targeting and fast delivery of stem cells can be achieved using the proposed non-invasive magnetic system.

2015-kubinova

Non-invasive magnetic system for the fast and targeted cell delivery into the lesion area.

Publication:  Tukmachev D, Lunov O, Zablotskii V, Dejneka A, Babic M, Sykova E, Kubinova S. An effective strategy of magnetic stem cell delivery for spinal cord injury therapy. Nanoscale. 2015;7(9):3954-8. IF 7.39

GAČR: 17-03765S. Paracrine effect of mesenchymal stem cell-secretome and its modification for the treatment of spinal cord injury. 2017-2019.

MŠMT, Centre of Reconstructive Neuroscience, NEURORECON, CZ.02.1.01/0.0/0.0/15_003/0000419, 2016-2022.

MŠMT: NPU I, LO1309 Cell Therapy and Tissue Repair. Duration: 2014-2019.

MPO TRIO: FV10081 Low temperature plasma for human medicine. Duration: 2016-2020.

MŠMT EATRIS LM2015064 16-19. Czech National Node of European Infrastructure in Translational Medicine. Duration: 2016-2019, www.umtm.cz/eatris

2017

Čejka, Č., Kössl, J., Heřmánková, B., Holáň, V., Čejková, J.: (2017) Molecular Hydrogen Effectively Heals Alkali-Injured Cornea via Suppression of Oxidative Stress. Oxidative Medicine and Cellular Longevity. 8906027.

Chudičková, M., Brůža, P., Zajícová, A., Trošan, P., Svobodová, L., Javorková, E., Kubinová, Š., Holáň, V.: (2017) Targeted neural differentiation of murine mesenchymal stem cells by a protocol simulating the inflammatory site of neural injury. Journal of Tissue Engineering and Regenerative Medicine. 11(5): 1588-1597.

Kočí, Z., Výborný, K., Dubišová, J., Vacková, I. , Jäger, A., Lunov, O., Jiráková, K., Kubinová, Š.: (2017) Extracellular Matrix Hydrogel Derived from Human Umbilical Cord as a Scaffold for Neural Tissue Repair and Its Comparison with Extracellular Matrix from Porcine Tissues. Tissue Engineering part C- Methods. 23(6): 333-345.

Kubinová, Š. , Závišková, K., Uherková, L., Zablotskyy, V., Churpita, O., Lunov, O., Dejneka, A.: (2017) Non-thermal air plasma promotes the healing of acute skin wounds in rats. Scientific Reports. 7: 45183.

Kubinová, Š.: (2017) Extracellular matrix based biomaterials for central nervous system tissue repair: the benefits and drawbacks. Neural Regeneration Research. 12 (9): 1430-1432.

Lunov, O., Zablotskyy, V., Churpita, O., Lunova, M., Jirsa, M., Dejneka, A., Kubinová, Š.: (2017) Chemically different non-thermal plasmas target distinct cell death pathways. Scientific Reports. 7: 600.

Lunova, M., Prokhorov, A., Jirsa, M., Hof, M., Olžyńska, A., Jurkiewicz, P., Kubinová, Š., Lunov, O., Dejneka, A.: (2017) Nanoparticle core stability and surface functionalization drive the mTOR signaling pathway in hepatocellular cell lines. Scientific Reports. 7(1): 16049.

Macková, H., Plichta, Z., Hlídková, H., Sedláček, O., Konefal, R., Sadakbayeva, Z., Dušková-Smrčková, M., Horák, D., Kubinová, Š.: (2017) Reductively Degradable Poly(2-hydroxyethyl methacrylate) Hydrogels with Oriented Porosity for Tissue Engineering Applications. ACS Applied Materials and Interfaces. 9 (12): 10544-10553.

Machová-Urdzíková, L., Růžička, J., Kárová, K., Kloudová, A., Svobodová, B. , Anubhav, A., Dubišová, J., Schmidt, M., Kubinová, Š., Jhanwar Uniyal, M., Jendelová, P.: (2017) A green tea polyphenol epigallocatechin-3-gallate enhances neuroregeneration after spinal cord injury by altering levels of inflammatory cytokines. Neuropharmacology. 126: 213-223.

Petrenko, Y. , Syková, E., Kubinová, Š.: (2017) The therapeutic potential of three-dimensional multipotent mesenchymal stromal cell spheroids. Stem Cell Research & Therapy. 8: 94.

Petrenko, Y.A. , Petrenko, A.Y., Martin.I, Wendt, D.: (2017) Perfusion bioreactor-based cryopreservation of 3D human mesenchymal stromal cell tissue grafts. Cryobiology. 76: 150-153.

Rogulska, O., Petrenko, Y., Petrenko, A.: (2017) DMSO-free cryopreservation of adipose-derived mesenchymal stromal cells: expansion medium affects post-thaw survival. Cytotechnology. 69(2):265-276.

Růžička, J., Machová Urdziková, L., Gillick, J., Amemori, T., Romayuk, N., Kárová, K., Závišková, K., Dubišová, J., Kubinová, Š., Murali, R., Syková, E., Jhanwar-Uniyal, M., Jendelová, P.: (2017) A Comparative Study of Three Different Types of Stem Cells for Treatment of Rat Spinal Cord Injury. Cell Transplantation. 26(4): 585-603.

Růžička, J., Machová Urdziková, L., Svobodová, B., Amin, A.G., Kárová, K., Dubišová, J., Závišková, K., Kubinová, Š., Schmidt,M., Jhanwar-Uniyal, M., Jendelová, P.: (2017) Combined therapy of Curcumin and Epigallocatechin gallate in an experimental model of spinal cord injury in rats. Neural Regeneration Research. IN PRESS

Uzhytchak, M., Lynnyk, A., Zablotskii, V., Dempsey, N.M., Dias, A. L., Bonfim, M., Lunova, M., Jirsa, M., Kubinová, Š., Lunov, O., Dejneka, A.: (2017) The use of pulsed magnetic fields to increase the uptake of iron oxide nanoparticles by living cells. Applied Physics Letters. 111: 243703.

Články v odborném recenzovaném periodiku bez IF:

Kubinová, Š., Závišková, K. , Lunov, O., Churpita, O., Zablotskyy, V. A., Dejneka, A.:(2016) Nízkoteplotní plazma v hojení ran. Kazuistiky v diabetologii. 1: 20-23.

2016

Čejka, Č., Čejková, J., Trošan, P., Zajícová, A., Syková, E., Holáň, V.: (2016) Transfer of mesenchymal stem cells and cyclosporine A on alkali-injured rabbit cornea using nanofiber scaffolds strongly reduces corneal neovascularization and scar formation. Histol. Histopath.,969-980.

Čejková, J., Čejka, Č., Trošan, P., Zajícová, A., Syková, E., Holáň, V.: (2016) Treatment of alkali-injured cornea by cyclosporine A-loaded electrospun nanofibers - An alternative mode of therapy. Exp. Eye Res., 147:128-37.

Forostyak, O., Butenko, O., Anděrová, M., Forostyak, S., Syková, E., Verkhratsky, A., Dayanithi, G.: (2016) Specific profiles of ion channels and ionotropic receptors define adipose- and bone marrow derived stromal cells. Stem Cell Res., 16(3): 622-634.

Forostyak, O., Forostyak, S., Kortus, Š., Syková, E., Verkhratsky, A., Dayanithi, G.: (2016) Physiology of Ca(2+) signalling in stem cells of different origins and differentiation stages. Cell Calcium, 59(2-3): 57-66.

Herynek, V., Turnovcová, K., Veverka, P., Dědourková, T., Žvátora, P., Jendelová, P., Gálisová, A., Kosinová, L., Jiráková, K., Syková, E.: (2016) Using ferromagnetic nanoparticles with low Curie temperature for magnetic resonance imaging-guided thermoablation. Int. J. Nanomed., 11: 3801-3811.

Chudičková, M. , Brůža, P., Zajícová, A., Trošan, P., Svobodová, L., Javorková, E., Kubinová, Š., Holáň, V.: (2015) Targeted neural differentiation of murine mesenchymal stem cells by a protocol simulating the inflammatory site of neural injury. J. Tissue Eng. Regen. Med., IN PRESS

Jelínek, M., Bačáková, L., Remsa, J., Kocourek, T., Mikšovský, J., Písařík, P.,Vandrovcová, M., Filová, E.,Kubinová, Š.: (2016) Hybrid Laser Technology for Creation of Doped Biomedical Layers. Journal of Materials Science and Chemical Engineering. 4 (1) 98-104.

Jendelová, P., Kubinová, Š., Sandvig, I., Erceg, S., Sandvig, A., Syková, E.: (2016) Current developments in cell - and biomaterial-based approaches for stroke repair. Expert Opin Biol Ther., 16(1): 43-56.

Jiráková, K., Šeneklová, M ., Jirak, D., Turnovcová, K., Vosmanská, M., Babič, M., Horák, D., Veverka, P., Jendelová, P.: (2016) The effect of magnetic nanoparticles on neuronal differentiation of iPS-derived neural precursors Int J Nanomedicine, 6: 6267-6281.

Kaman, O., Dědourková, T., Koktan, J., Kuličková, J., Maryško, M., Veverka, P., Havelek, R., Královec, K., Turnovcová, K., Jendelová, P., Schröfel, A., Svoboda, L.: (2016) Silica-coated manganite and Mn-based ferrite nanoparticles: a comparative study focused on cytotoxicity. J. Nanopart. Res., 18 (4): 100.

Kortus, Š., Srinivasan, C., Forostyak, O., Ueta, Y., Syková, E., Chvátal, A., Zápotocký, M., Verkhratsky, A., Dayanithi, G.: (2016) Physiology of spontaneous [Ca2+]i oscillations in the isolated vasopressin and oxytocin neurones of the rat supraoptic nucleus. Cell Calcium., 59(6): 280-288.

Kortus, Š., Srinivasan, C., Forostyak, O., Zápotocký, M., Ueta, Y., Syková, E., Chvátal, A., Verkhratsky, A., Dayanithi, G.: (2016) Sodium-calcium exchanger and R-type Ca2+ channels mediate spontaneous [Ca2+]i oscillations in magnocellular neurones of the rat supraoptic nucleus. Cell Calcium., 59(6): 289-298.

Lukovic, D., Diez Lloret, A., Stojkovic, P., Rodríguez-Martínez, D., Perez Arago, M.A., Rodriguez-Jimenez F.J., González-Rodríguez, P., López-Barneo, J., Syková, E., Jendelová, P., Kostic, J., Moreno-Manzano, V., Stojkovic,M., Shomi S Bhattacharya, S.S, Erceg S.: (2016) Highly efficient neural conversion of human pluripotent stem cells in adherent and animal-free conditions. Stem Cells Transl. Med., IN PRESS

Lukovic, D., Moreno-Manzano, V., Rodriguez-Jimenez, F.J., Vilches, A., Syková,E., Jendelová, P.,Stojkovic, M., Erceg S.: (2016) hiPSC Disease Modeling of Rare Hereditary Cerebellar Ataxias: Opportunities and Future Challenge. Neuroscientist ,IN PRESS

Lunov, O., Zablotskii, V., Churpita, O., Jaeger, A., Polívka, L ., Syková, E., Terebová, N., Kulikov, A., Kubinová, Š., Dejneka, A.: (2016) Towards the understanding of non-thermal air plasma action: effects on bacteria and fibroblasts. RSC Adv., 6(30) 25286-25292.

Lunov, O., Zablotskii, V., Churpita, O., Jäger, A., Polívka, L., Syková, E., Dejneka, A., Kubinová, Š.: (2016) The interplay between biological and physical scenarios of bacterial death induced by non-thermal plasma. Biomaterials., 82: 71-83.

Lunova, M., Zablotskii, V., Dempsey, N.M., Devillers, T., Jirsa, M., Syková, E., Kubinová, Š., Lunov, O., Dejneka, A.: (2016) Modulation of collective cell behaviour by geometrical constraints. Integr. Biol., 8(11): 1099-1110.

Macková, H., Plichta, Z., Proks, V., Kotelnikov, I., Kučka, J., Hlídková, H., Horák, D., Kubinová, Š, Jiráková, K.: (2016) RGDS- and SIKVAVS-Modified Superporous Poly(2-hydroxyethyl methacrylate) Scaffolds for Tissue Engineering Applications. Macromol. Biosci., IN PRESS.

Machová Urdzíková, L., Kárová, K., Růžička, J., Kloudová, A., Shannon, C., Dubišová, J., Murali, R., Kubinová, Š., Syková, E., Jhanwar-Uniyal, M., Jendelová, P.: (2016) The Anti-Inflammatory Compound Curcumin Enhances Locomotor and Sensory Recovery after Spinal Cord Injury in Rats by Immunomodulation. Int. J. Mol. Sci., 17(1).

Novotná, B., Turnovcová, K., Veverka, P., Rössner, P. Jr., Bagryantsevá, Y., Herynek, V., Zvatora, P., Vosmanská, M., Klementová, M., Syková, E., Jendelová, P.: (2016) The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells. Nanotoxicology, 10(6): 662-670.

Růžička, J., Kulijewicz-Nawrot, M., Rodrigez-Arellano, J.J., Jendelová,P., Syková, E.: (2016) Mesenchymal Stem Cells Preserve Working Memory in the 3xTg-AD Mouse Model of Alzheimer's Disease. Int. J. Mol. Sci., 17(2): 152.

Růžička, J., Machová Urdziková, L ., Gillick, J., Amemori, T., Romayuk, N., Kárová, K., Závišková, K., Dubišová, J., Kubinová, Š., Murali, R., Syková, E., Jhanwar-Uniyal, M., Jendelová, P.: (2016) A comparative study of three different types of stem cells for treatment of rat spinal cord injury. Cell Transplant., IN PRESS

Syková, E ., Rychmach, P., Drahorádová, I., Konrádová, Š., Růžičková, K., Voříšek, I., Forostyak, S., Homola, A., Bojar, M.: (2016) Transplantation of mesenchymal stromal cells in patients with amyotrophic lateral sclerosis: Results of Phase I/IIa clinical trial. Cell Transplant., IN PRESS

Školoudik, L., Chrobok, V., Kalfert, D., Koči, Z., Syková, E., Chumak, T., Popelář, J., Syka. J., Laco, J., Dědková, J., Dayanithi, G., Filip, S.: (2016) Human multipotent mesenchymal stromal cells in the treatment of postoperative temporal bone defect: an animal model. Cell Transplant., 25(7): 1405-1414.

Šponer, P., Filip, S., Kučera, T., Brtková, J., Urban,K., Palička, V., Kočí, Z., Syka, M., Bezrouk, A., Syková, E.: (2016) Utilizing Autologous Multipotent Mesenchymal Stromal Cells and -Tricalcium Phosphate Scaffold in Human Bone Defects: A Prospective, Controlled Feasibility Trial. Biomed Res. Int., 2076061.

Tukmachev, D., Forostyak, S., Kočí, Z., Závišková, K., Vacková, I., Výborný, K., Sandvig, I., Sandvig, A., Medberry, C.J., Badylak, S.F., Syková, E., Kubinová, Š.: (2016) Injectable Extracellular Matrix Hydrogels as Scaffolds for Spinal Cord Injury Repair. Tissue Eng., 22(3-4): 306-317.

Voříšek, I., Syka, M., Vargová, L.: (2016) Brain Diffusivity and Structural Changes in the R6/2 Mouse Model of Huntington Disease. J. Neurosci. Res., IN PRESS.

Zablotskii, V., Lunov, O., Kubinová, Š., Polyaková, T., Syková, E., Dejneka, A.: (2016) Effects of high-gradient magnetic fields on living cell machinery. J. Phys. D-Appl. Phys., 49: 493003.

Články v odborném recenzovaném periodiku bez IF:

Kubinová Š. New trends in spinalcordtissueengineering. FutureNeurol. 2015; 10 (2):129-145.

Kubinová Š, Drahošová M, Bezděk D, Churpita O, Musil R, Hanzálek T, Soldánová M, Berka P, Dejneka A. Nízkoteplotní plazma ve veterinární medicíně: Kazuistiky. Veterinární lékař. 2016; 14(2):69-75.

Vacková I, Kubinová Š. Stem cell conditioned medium for cell-free therapies. Československá fyziologie. 2016; 65(1):25-31.

2015

Amemori, T., Jendelová, P., Růžička, J., Urdziková, L.M., Syková, E.: (2015) Alzheimer's Disease: Mechanism and Approach to Cell Therapy. Int J Mol Sci. 16(11): 26417-26451.

Amemori, T., Růžička, J., Romanyuk, N., Jhanwar-Uniyal, M., Syková, E., Jendelová, P.: (2015) Comparison of intraspinal and intrathecal implantation of induced pluripotent stem cell-derived neural precursors for the treatment of spinal cord injury in rats. Stem Cell Res Ther. 6(1): 257.

Babič, M., Schmiedtová, M., Poledne, R., Herynek V., Horák D.: (2015) In vivo monitoring of rat macrophages labeled with poly(l-lysine)-iron oxide nanoparticles. J Biomed Mater Res B Appl Biomater. 103(6): 1141-1148.

Forostyak, O., Romanyuk, N., Verkhratsky, A., Syková, E., Dayanithi G.: (2015) Plasticity of calcium signaling cascades in human embryonic stem cell-derived neural precursors. Stem Cells Dev., 22(10): 1506-1521.

Havlas, V., Kotaška, J., Koníček, P., Trč, T., Konrádová, Š., Kočí, Z., Syková, E.: (2015) Use of cultured human autologous bone marrow stem cells in repair of a rotator cuff tear: preliminary results of a safety study. Acta Chir Orthop Traumatol Cech. 82(3): 229-234.

Hejtčl, A., Jendelová, P., Sameš, M., Syková, E.: (2015) Experimental Treatment of Spinal Cord Injuries. Česká A Slovenska neurologie a neurochirurgie 78(4): 377-392.

Jelínek, M., Kocourek, T., Zemek, J., Mikšovský, J., Kubinová, Š., Remsa, J., Kopeček, J., Jurek, K.: (2015) Chromium-doped DLC for implants prepared by laser-magnetron deposition. Mater. Sci. Eng. C-Mater. Biol. Appl. 46: 381-386.

Klíma, K., Vaněček, V., Kohout, A., Jiroušek, O., Foltán, R., Stulík, J., Machoň, V., Pavlíková, G., Jendelová, P., Syková, E., Šedý, J.: (2015) Stem cells regenerative properties on new rat spinal fusion model. Physiol. Res. 64(1): 119-128.

Kubinová, Š., Horák, D., Hejčl, A., Plichta, Z., Kotek, J., Proks, V., Forostyak, S., Syková, E.: (2015) SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair. J Tissue Eng Regen Med. 9(11): 1298-1309.

Kwiecien, J. M., Jarosz, B., Machová-Urdziková, L., Rola, R., Dabrowski, W.: (2015) Subdural infusion of dexamethasone inhibits leukomyelitis after acute spinal cord injury in a rat model. Folia Neuropathol., 64(1): 41-45.

Lukovic, D., Moreno-Manzano, V., Lopez-Mocholi, E., Rodriguez-Jiménez, F.J., Jendelová, P., Syková, E., Oria, M., Stojkovic, M., Erceg, S.: (2015) Complete rat spinal cord transection as a faithful model of spinal cord injury for translational cell transplantation. Sci Rep., 5: 19640.

Lukovic, D., Stojkovic, M., Moreno-Manzano, V., Jendelová, P., Syková, E., Bhattacharya, S. S., Erceg S.: (2015) Reactive astrocytes and stem cells in spinal cord injury: good guys or bad guys?Stem Cells. 33(4) :1036-1041.

Lunov, O., Churpita, O., Zablotskii, V., Deyneka, I.G., Meshkovskii, I.K., Jäger, A., Syková, E., Kubinová,Š., Dejneka, A.: (2015) Non-thermal plasma mills bacteria: Scanning electron microscopy observations. Applied Physics Letters. 106 (5) : 053703.

Raha-Chowdhury, R., Raha, A.A., Forostyak, S., Zhao, J.W., Stott, S.R., Bomford, A.: (2015) Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci., 16: 24.

Romanyuk, N., Amemori, T., Turnovcová, K., Procházka, P., Onteniente, B., Syková, E., Jendelová, P.: (2015) Beneficial effect of human induced pluripotent stem cell-derived neural precursors in spinal cord injury repair.Cell Transplant.24(9): 1781-1797.

Syka, M., Keller, J., Klempíř, J., Rulseh, A.M., Roth, J., Jech, R., Vořišek, I., Vymazal, J.: (2015) Correlation between relaxometry and diffusion tensor imaging in the globus pallidus of Huntington's disease patients. PLoS One., 10(3): e0118907.

Tukmachev, D. , Lunov, O., Zablotskii, V., Dejneka, A., Babič, M., Syková, E., Kubinová, Š.: (2015) An effective strategy of magnetic stem cell delivery for spinal cord injury therapy. Nanoscale.7(9): 3954-3958.

2014

Machová-Urdziková L, Růžička J, LaBagnara M, Kárová K, Kubinová Š, Jiráková K, Murali R, Syková E, Jhanwar-Uniyal M, Jendelová P. Humanmesenchymal stem cellsmodulateinflammatorycytokinesafterspinalcordinjury in rat. Int J Mol Sci. 2014; 15(7):11275-93. IF 2.862

Zablotskii V, Lunov O, Novotná B, Churpita O, Trošan P, Holáň V, Syková E, Dejneka A, Kubinová Š. Down-regulationofadipogenesisofmesenchymal stem cells by oscillatinghighgradientmagneticfields and mechanicalvibration. ApplPhysLett. 2014; 105, 103702. IF 3.302

Kočí Z, Turnovcová K, Dubský M, Baranovičová L, Holáň V, Chudíčková M, Syková E, Kubinová S. Characterizationofhumanadiposetissue-derivedstromalcellsisolatedfromdiabeticpatient'sdistallimbswithcriticalischemia. Cell BiochemFunct. 2014;32(7):597-604. IF 2.005

Lunov O, Zablotskii V, Churpita O, Chanova E, Sykova E, Dejneka A, Kubinova S, Cell deathinduced by ozone and various non-thermalplasmas: therapeuticperspectives and limitations, SciRep. 2014;4 : 7129. IF 5.578

Institute of Physics of the Czech Academy of Sciences

Institute of Macromolecular Chemistry of the Czech Academy of Sciences

Institute for Clinical and Experimental Medicine

Biomedical Center of the Faculty of Medicine in Pilsen

Patents

2017
Petrenko, Y., Syková, E., Čejková, J., Vacková, I., Groh, T.: (2017) Prostředek pro uchování, transport a aplikaci kmenových buněk. (A device for storage, transport and application of stem cells.) Vlastník/Owner: Ústav experimentální medicíny AV ČR, v. v. i.
Číslo patentu/Number: 306800

Užitné vzory / Utility patterns

2018

Čejková, J., Čejka, Č., Kubinová, Š., Vacková, I.: (2018) Přípravek k léčení zánětlivých a degenerativních onemocnění oka a oční kapky adjustované ve vialkách (EN: A preparation for the treatment of inflammatory and degenerative eye diseases and eye drops adjusted in vials) Vlastník: Ústav experimentální medicíny AV ČR, v.v.i.
Číslo vzoru/Number: 31773

2017

Groh, T.,  Petrenko, Y., Kosnarová, E., Bauer, P.: (2017) Prostředek pro uchování lidských nebo zvířecích buněk při velmi nízkých teplotách (EN: A preparation for preserving human or animal cells at very low temperatures). Vlastník/Owner: Bioinova, s.r.o., Ústav experimentální medicíny AV ČR, v. v. i.
Číslo vzoru/Number: 31206

Syková, E., Čejková, J., Čejka, Č.,  Forostyak, S.: (2017) Prostředek obsahující kmenové buňky k léčení zánětlivých onemocnění, posttraumatických reakcí a degenerativních onemocnění. (EN: A preparation containing stem cells for treatment of inflammatory diseases, post-traumatic reactions and degenerative diseases.) Vlastník/Owner: Ústav experimentální medicíny AV ČR, v.v. i.
Číslo vzoru/Number: 30239

Petrenko, Y., Syková, E., Čejková, J., Vacková, I., Groh, T.: (2017) Prostředek pro uchování, transport a aplikaci kmenových buněk. (A means for storage, transportation and application of stem cells.) Vlastník/Owner: Ústav experimentální medicíny AV ČR, v. v. i.
Číslo vzoru/Number: 30270

Churpita, O., Dejneka, A., Kubinová, Š. : (2017) Zdroj nízkoteplotního plazmatu, zejména pro generaci plazmatu při využití v medicínských bioaplikacích (EN: A low-temperature plasma source, especially for plasma generation when used in medical bio-applications) Vlastník/Owner: Fyzikální ústav AV ČR, v.v.i., Ústav experimentální medicíny AV ČR, v.v.i.
Číslo vzoru/Number: 310034

Ověřená technologie / Verified technology:

Vacková, I., Turnovcová, K.: (2016) Proces izolace mezenchymálních stromálních buněk z kryoprezervované lidské pupečníkové tkáně. (The process of isolation of mesenchymal stromal cells from cryopreserved human umbilical cord tissue.)

Vacková, I., Turnovcová, K., Růžičková, K, Holáň, V.: (2016) Optimální koncentrace mezenchymálních stromálních buněk při kryokonzervaci. (The optimal concentration of mesenchymal stromal cells during cryopreservation.)                                                        

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