The department is focused on the evaluation of toxic effects of engineered nanoparticles and combustion generated ultrafine particles in the human cell lines that originate from the surface epithelium of the respiratory tract as well as in the 3D human airway epithelia models reconstituted in vitro (MucilAirTM system). Another topic includes molecular epidemiology studies analyzing the effects of environmental pollution on human health.
The methods include various tests of cytotoxicity, the detection of reactive oxygen species and oxidative damage of DNA, proteins and lipids. Direct genotoxicity is analyzed using Comet assay and by detection of DNA adducts. Structural and numerical chromosomal aberrations are evaluated by micronucleus test and by flusorescent staining (FISH). The toxicogenomic methods are used for evaluation of the effects of nanoparticles on gene expression levels or DNA methylation changes. Analyses of protein expression and DNA repair activity are also performed. The aim of the research is to assess the toxicity of nanoparticles for biomedicine applications, and the evaluation of the potential adverse effects on human health in employees professionally exposed to nanomaterials and in the common population exposed to air pollution.
The research of the departent is further focused on the study of stem cells and thei rrole in the healing and regeneration of damaged organs and tissues. Stem cells are isolated from various organs, propagated in tissue cultures, characterized and transferred into the site of tissue damage or inflammation. Various types of nanofiber scaffolds are used for the transfer of stem cell into the site of damage. Stem cells are used in experimental models for the treatment of severe damages of ocular surface or in attempts to treat so far incurable degenerative diseases of the retina. The final aim of these studies is recognition and characteization of mechanisms of therapeutic effects of stem cells and demonstration of their potential use for the impovement of of human life.
Deputy head:
RNDr. Michal Šíma, Ph.D.
E-mail: michal.sima@iem.cas.cz
Tel.: +420 241 062 669
Research scientists:
Pavel Rössner, Ph.D.
Antonín Ambrož, Ph.D.
Barbora Heřmánková, Ph.D.
Vladimír Holáň DrSc.
Eliška Javorková, Ph.D.
Helena Líbalová, Ph.D.
Andrea Rössnerová, Ph.D.
Michal Šíma, Ph.D.
Alena Zajícová, Ph.D.
PhD students:
Tereza Červená, MSc.
Barbora Echalar, MSc.
Kateřina Palacká, MSc.
Jitka Pavlíková, MSc.
Zuzana Šímová, MSc.
Research assistants:
Fatima Elzeinová, MSc.
Zdeněk Novák, MSc.
Zuzana Nováková, MSc.
Anežka Vimrová, MSc.
Kristýna Vrbová, MSc.
Technicans:
Lucie Holáňová
Important results in 2022
1. The impact of extractable organic matter from gasoline and alternative fuel emissions on bronchial cell models (BEAS-2B, MucilAir™)
Air pollution caused by road traffic has an unfavorable impact on the environment and also on human health. We focused on extractable organic matter (EOM) from particulate matter from fuels with different ethanol content. We performed cytotoxicity evaluation, quantification of mucin and extracellular reactive oxygen species production, DNA breaks detection, and selected gene deregulation analysis, after one and five days of exposure of human bronchial epithelial model (BEAS-2B) and a 3D model of the human airway (MucilAir™). Our data suggest that the longer exposure had more pronounced effects on the parameters of cytotoxicity and mucin production, while the impacts on ROS generation and DNA integrity were limited.
Number of genes with specific deregulation when samples after 5-day exposure were compared with samples obtained from 1-day exposure.
In MucilAir™ no significant differences were detected for E5 between 5-day and 1-day exposure, this EOM caused a differential expression of 13 genes in BEAS-2B cells. The expression of five and eight genes differed after exposure to E20 EOM in MucilAir™ or BEAS-2B cells, respectively, when the 5-day and 1-day treatment was compared.
Publications:
Sima M, Cervena T, Elzeinova F, Ambroz A, Beranek V, Vojtisek-Lom M, Klema J, Ciganek M, Rossner P Jr. The impact of extractable organic matter from gasoline and alternative fuel emissions on bronchial cell models (BEAS-2B, MucilAir™). Toxicol In Vitro. 2022 Apr;80:105316. doi: 10.1016/j.tiv.2022.105316. Epub 2022 Jan 21. PMID: 35066112.
2. Oxidative Stress and Antioxidant Response in Populations of the Czech Republic Exposed to Various Levels of Environmental Pollutants
The publication discusses an occupational exposure affecting oxidative stress in policemen living in diverse city localities differing by air pollution. Personal characteristics, levels of air pollutants, antioxidant enzymes, pro-inflammatory cytokines, persistent organic pollutants in plasma, as well as urinary metabolites of polycyclic aromatic hydrocarbons were investigated as parameters potentially associated with markers of DNA oxidation (8-oxodG) and lipid peroxidation (15-F2t-IsoP). In conclusion, oxidative damage was associated with inflammation, however, a variable responsible for pro-inflammatory response was not identified.
Multivariate estimates of associations between oxidative stress markers and selected parameters.
Urinary 8-oxodG levels increased with decreasing antioxidant capacity (ORAC) and increasing plasma concentration of IL-6, a pro-inflammatory cytokine. In addition, a borderline positive association of GPx activity with 8-oxodG was noted. The effect of environmental pollutants on oxidatively damaged DNA was minor, as concentrations of only two compounds (o,p‘-DDE, BDE 154) were found to be associated with the excretion of 8-oxodG to urine. Lipid peroxidation was influenced by the activity of CAT and plasma concentration of IL-6. Exposure to tobacco smoke was another parameter associated with the levels of 15-F2t-IsoP. Finally, the effects of persistent organic pollutants were identified: BDE 154 was negatively linked with lipid peroxidation, while for plasma BDE 99 levels, a positive correlation was observed.
Publications:
Ambroz A, Rossner P Jr, Rossnerova A, Honkova K, Milcova A, Pastorkova A, Klema J, Pulkrabova J, Parizek O, Vondraskova V, Zelenka J, Vrzáčková N, Schmuczerova J, Topinka J, Sram RJ. Oxidative Stress and Antioxidant Response in Populations of the Czech Republic Exposed to Various Levels of Environmental Pollutants. Int J Environ Res Public Health. 2022 Mar 18;19(6):3609. doi: 10.3390/ijerph19063609. PMID: 35329296; PMCID: PMC8955578.
3. Studies of the effect of metal nanoparticles on phenotype and function properties of mesenchymal stem cells.
Mesenchymal stem cells (MSCs) were isolated from mouse adipose tissue and were cultured with four types of metal nanoparticles (ZnO, Ag, CuO, TiO2). The effect of these nanoparticles on the phenotype, metabolic activity, differentiation potential, gene expression and production of cytokines was tested. All types of nanoparticles exerted negative effects on of MSCs. However, apparent differences in the impact of individual types of nanoparticles on various characteristics of MSCs were observed.
Isolation of mesenchymal stem cells and their incubation with nanoparticles.
Mesenchymal stem cells were isolated from adipose tissue, cultured for 14 days and characterized. Then, the cells were incubated for 48 hours with metal nanoparticles and the impacts of nanoparticles of phenotypic and function properties of cells were characterized.
Publications:
Echalar B., Dostalová D., Palacká K., Javorková E., Červena T., Zajícová A., Holáň V., Rössner P.: Effects of antibacterial metal nanoparticles on characteristics and functions of mouse mesenchymal stem cells. Toxicology In Vitro, in press. Accepted.
Important results in 2021
1. Markers of lipid oxidation and inflammation in bronchial cells exposed to complete gasoline emissions and their organic extracts
We investigated the inflammation-related markers in human bronchial epithelial cells and a 3D model of the human airways after exposure to complete emissions and extractable organic matter from these emissions from two gasoline types. Exposure of the 3D model to emissions from ordinary gasoline induces the pro-inflammatory response. This observation highlights the potential negative impacts of ordinary gasoline, while the effects of an alternative fuel (with higher ethanol content) are relatively weak.
A comparison of production of immune response-relevant molecules by MucilAir™ and BEAS-2B cells exposed to complete emissions and EOMs from complete emissions from E5 and E20 fuels at time points T1 and T5.
A - Production of immune response-relevant molecules by MucilAir™ and BEAS-2B cells exposed to complete emissions from E5 and E20 fuels at time points T1 and T5. B - Production of immune response-relevant molecules by MucilAir™ and BEAS-2B cells exposed to EOMs from complete emissions from E5 and E20 fuels at time points T1 and T5. Red and green colors denote increased and decreased levels of the respective marker, when compared with the control at the individual time point. ND, not detectable.
Publications:
Rossner P, Cervena T, Vojtisek-Lom M, Neca J, Ciganek M, Vrbova K, Ambroz A, Novakova Z, Elzeinova F, Sima M, Simova Z, Holan V, Beranek V, Pechout M, Macoun D, Rossnerova A, Topinka J. Markers of lipid oxidation and inflammation in bronchial cells exposed to complete gasoline emissions and their organic extracts. Chemosphere. 2021 Oct;281:130833. doi: 10.1016/j.chemosphere.2021.130833.
2. Testing strategies of the in vitro micronucleus assay for the genotoxicity assessment of nanomaterials in BEAS-2B cells
We tested the genotoxicity of five types of nanomaterials using four variants of micronuclei protocols, differing in the time of exposure and the application of cytochalasin-B combined with the simultaneous and delayed co-treatment with nanomaterials. The comparison of the testing strategies revealed that the level of DNA damage induced by nanomaterials is affected by the selected methodological approach. This fact should be considered in the interpretation of the results of genotoxicity tests.
Microscopic analysis of BEAS-2B cells cultivated with various nanomaterials.
Examples of binucleated cells without (A) or with micronuclei (B, C) with tested nanomaterials (red arrows) as detected by the optical microscope: A - TiO2, concentration 10 µg/mL, 28 h co-treatment with cytochalasin-B; B - SiO2, concentration 1 µg/mL, 48 h delayed co-treatment with cytochalasin-B; C - Ag, concentration 25 µg/mL, 28 h co-treatment with cytochalasin-B.
Publications:
Cervena T, Rossnerova A, Zavodna T, Sikorova J, Vrbova K, Milcova A, Topinka J, Rossner P Jr. Testing Strategies of the In Vitro Micronucleus Assay for the Genotoxicity Assessment of Nanomaterials in BEAS-2B Cells. Nanomaterials (Basel). 2021 Jul 27;11(8):1929. doi: 10.3390/nano11081929.
Important results in 2020
1. Gene Expression and Epigenetic Changes in Mice Following Inhalation of Copper(II) Oxide Nanoparticles
The transcriptomic response and epigenetic changes in murine lungs were investigated using next generation sequencing after exposure to CuO nanoparticles for four various time periods. The inhalation of CuO NPs affected both mRNA and miRNA expression. A significant transcriptomic response was observed already after 3 days of exposure. The affected biological processes and pathways indicated the negative impacts on the immune system and potential role in carcinogenesis.
The distribution of common and unique genes differentially expressed after copper(II) oxide nanoparticles (CuO NPs). Venn diagram indicates the number of common/unique differentially expressed genes after mouse exposure to CuO nanoparticles in four various time periods. The common deregulation for all exposure conditions was detected for two genes. In contrast, most unique deregulated genes were observed after three months exposure.
Publications:
Rossner, P., Vrbova, K., Rossnerova, A., Zavodna, T., Milcova, A., Klema, J., Vecera, Z., Mikuska, P., Coufalik, P., Capka, L., Krumal, K., Docekal, B., Holan, V., Machala, M., Topinka, J.: (2020) Gene expression and epigenetic changes in mice following inhalation of copper(II) oxide nanoparticles. Nanomaterials. 10(3): 550. doi: 10.3390/nano10030550. IF= 4.034
2. Genotoxicant exposure, activation of the aryl hydrocarbon receptor, and lipid peroxidation in cultured human alveolar type II A549 cells
We investigated the processes associated with lipid peroxidation in human alveolar epithelial cells (A549) exposed for 4 h or 24 h to model polycycklic aromatic hydrocarbons (PAH) (benzo[a]pyrene, BaP; 3- nitrobenzanthrone, 3-NBA) and organic extracts from ambient air particulate matter (EOM), collected in two seasons in a polluted locality. In summary, our data suggest that EOM, and partly BaP, reduce lipid peroxidation by a mechanism that involves aryl hydrocarbon receptor-dependent inhibition of prostaglandin endoperoxide synthase 2 expression.
Selected processes related to interaction of the aryl hydrocarbon receptor (AhR) with lipid peroxidation. The following parameters were analyzed in our study: expression of genes activating PAH [cytochrome P450 1A1 (CYP1A1), 1B1 (CYP1B1)]; prostaglandin-endoperoxide synthase-2 (PTGS- 2) expression; levels of AA and 15-F2t-isoprostane (IsoP) as a marker of lipid peroxidation; activity of NF-κB. PAH/EOM are metabolized via AhR-dependent (CYP1A1, CYP1B1) or AhR-independent mechanism and may cause reactive oxygen species (ROS) generation. ROS attack AA and induce its conversion to IsoP either non-enzymatically, or via a PTGS-2-catalyzed mechanism, in which AA is first removed from the membrane by the activity of phospholipase A2 (PLA2). AhR activation may further induce or inhibit PTGS-2 expression, thus affecting lipid peroxidation levels. ROS activate NF-κB transcription factor that regulates numerous cellular processes including PTGS-2 or CYP expression. This transcription factor may also be activated by AhR-dependent mechanism involving CYP expression.
Publications:
Rossner, P., Libalova, H., Vrbova, K., Cervena, T., Rossnerova, A., Elzeinova, F., Milcova, A., Novakova, Z., Topinka, J. (2020): Genotoxicant exposure, activation of the aryl hydrocarbon receptor, and lipid peroxidation in cultured human alveolar type II A549 cells. Mutat Res. 853: 503173. doi: 10.1016/j.mrgentox.2020.503173. IF= 2.506
Important results in 2019
1. Benzo[a]pyrene is associated with dysregulated myelo-lymphoid hematopoiesis in asthmatic children
Exposure to B[a]P might contribute to concurrent suppression of pro-inflammatory (e.g. NF-κB mediated Natural Killer T cells), and activation of anti-inflammatory pathways (e.g. IL10-secreting CD8+ T cells) in the urban asthmatic children. In addition, B[a]P appears to elevate heme biosynthesis, which in turn, promotes neutrophilic metamyelocyte expansion and reduction of CD71+ erythroid cells.
Overview of integrative network analysis
Publications:
Choi, H., Song, WM., Wang, M., Dostal, M., Pastorkova, A., Libalova, H., Tulupova, E., Rossnerova, A., Rossner, P. Jr., Sram, RJ., Zhang, B. (2019): Benzo[a]pyrene is associated with dysregulated myelo-lymphoid hematopoiesis in asthmatic children. Environ Int. 128: 218-232. doi: 10.1016/j.envint.2019.04.052. IF=7.943
2. The biological effects of complete gasoline engine emissions exposure in a 3D human airway model (MucilAirTM) and in human bronchial epithelial cells (BEAS-2B)
The aim of this study was real-time exposure of complete combustion emissions in two cell models. The models used were the BEAS-2B lung epithelial cell line and the MucilAirTM 3D lung model. To allow direct contact of combustion emissions and cells, both models were cultured on porous membranes. A unique exposure system has been created to allow real time direct expose to diluted exhaust gases. One-day and five-day repeated exposure showed higher tolerance and more realistic results in complex 3D models than commonly used lung cells.
BEAS-2B lung epithelial cell line and the MucilAirTM 3D lung model
Publications:
Rossner, P., Jr., Cervena, T., Vojtisek-Lom, M., Vrbova, K., Ambroz, A., Novakova, Z., Elzeinova, F., Margaryan, H., Beranek, V., Pechout, M., Macoun, D., Klema, J., Rossnerova, A., Ciganek, M., Topinka, J.: (2019) The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAirTM) and in Human Bronchial Epithelial Cells (BEAS-2B). International Journal of Molecular Sciences. 20(22). pii: E5710. doi: 10.3390/ijms20225710. IF=4.331
3. Study of therapeutic potential of stem cells for the treatment of degenerative retinal diseases
Degenerative retinal diseases represent the main cause of a decreased quality of vision or even blindness. A perspective for the treatment of these so far uncurable diseases is offered by mesechymal stem cells (MSCs). We showed that MSCs are potent producers of numerous growth and trophic factors supporting survival of retinal cells, inhibit harmful inflammatory reactions in the eye and are able to differentiate into cells expressing markers and properties of retinal cells.
The main mechanisms of the therapeutic effect of mesenchymal stem cells.
Publications:
Heřmánková, B., Kössl, J., Boháčová, P., Javorková, E., Hájková, M., Krulová, M., Zajícová, A., Holáň, V.: The immunoregulatory potential of mesenchymal stem cels in a retinal inflammatory environment. Stem Cell Rev. Rep., DOI 10,1007/s12015-019-09908-0, 2019.
Holáň, V., Heřmánková, B, Krulová, M., Zajícová, A.: Cytokine interplay among the diseased retina, inflammatory cells and mesenchymal stem cells – a clue to stem cell-based therapy. World J. Stem Cells 11, 957-967, 2019.
Important results in 2018
1. Transcriptional response to organic compounds from diverse gasoline and biogasoline fuel emissions in human lung cells
We collected and characterized gasoline exhaust particles (GEPs) produced by Gasoline Direct Injection (GDI) engine running neat gasoline fuel (E0) and its blends with 15% ethanol (E15), 25% n-butanol (n- But25) and 25% isobutanol (i-But25). To study the toxic effects of organic compounds extracted from GEPs, we analyzed gene expression profiles in human lung BEAS-2B cells. Our results indicate that i-But25 extract possessed the weakest genotoxic potency possibly due to the low PAH content.
Scheme of the study evaluating the transcriptional response to organic compounds from four diverse gasoline and biogasoline fuel emissions in human lung epithelial cells BEAS-2B.
Publications:
Libalova, H., Rossner, P. Jr., Vrbova, K., Brzicova, T., Sikorova, J., Vojtisek-Lom, M., Beranek, V., Klema, J., Ciganek, M., Machala, M., Topinka, J.: (2018) Transcriptional response to organic compounds from diverse gasoline and biogasoline fuel emissions in human lung cells. Toxicology in Vitro. 48: 329-341. doi: 10.1016/j.tiv.2018.02.002. IF=3.067
2. Gene expression profiling in healthy newborns from diverse localities of the Czech Republic
We analyzed whole genome expression in cord blood leukocytes of 202 newborns from the Czech Republic, differing localities and levels of air pollution. We aimed to identify differentially expressed genes (DEGs) and pathways in relation to locality. The highest number of DEGs was found in samples from Karvina. A pathway analysis revealed a deregulation of processes associated with cell growth, apoptosis or cellular homeostasis, immune response-related processes or oxidative stress response.
Heatmap of the most differentially expressed genes
Publications:
Honkova, K., Rossnerova, A., Pavlikova, J., Svecova, V., Klema, J., Topinka, J., Milcova, A., Libalova, H., Choi, H., Veleminsky, M., Sram, R.J., Rossner, P. Jr.: (2018) Gene Expression Profiling in Healthy Newborns from Diverse Localities of the Czech Republic. Environmental and Molecular Mutagenesis.59: 401-415; doi: 10.1002/em.22184. IF=2.528
3. Inhalation of ZnO nanoparticles: splice junction expression and alternative splicing in mice
Biological effects of ZnO nanoparticles inhalation were investigated in mice. The inhalation affected splice junctions expression in genes participating in oxidative stress, apoptosis, immune response, inflammation and DNA repair. Further, alternative splicing in oxidative stress and inflammation-related genes was affected. In summary, ZnO nanoparticles have a potential negative effect on biological systems.
mRNA expression levels assessed by RT-qPCR for selected genes.
Publications:
Rössner, Jr.P., Vrbová, K., Strapáčová, S., Rössnerová, A., Ambrož, A., Brzicová, T., Libalová, H., Javorková, E., Kulich, P., Vecera, Z., Mikuska, P., Coufalik, P., Krumal, K., Capka, L., Docekal, B., Moravec, P., Sery, O., Misek, I., Fictum, P., Fiser, K., Machala, M., and Topinka, J.: (2018) Inhalation of ZnO nanoparticles: splice junction expression and alternative splicing in mice. Toxicological Sciences. 168(1): 190-200. doi: 10.1093/toxsci/kfy288. IF=3.654
Important results in 2017
1. Ultrafine and Fine Particles and Hospital Admissions in Central Europe. Results from the UFIREG Study
We investigated effects of ultrafine and fine particulate matter on cause-specific hospital admissions in Central and Eastern European cities. We found that a 2,750 particles/cm3 increase of ultrafine particles caused an increase in the risk of respiratory hospital admissions. We also found increases in the risk of cardiovascular and respiratory admissions associated with exposure to particulate matter of aerodynamic diameter < 2.5 µm.
Praticles size and their deposition in the respiratory tract ( http://www.ufireg-central.eu).
Publikace:
Lanzinger S, Schneider A, Breitner S, Stafoggia M, Erzen I, Dostal M, Pastorkova A, Bastian S, Cyrys J, Zscheppang A, Kolodnitska T, Peters A; UFIREG study group: (2016) Ultrafine and Fine Particles and Hospital Admissions in Central Europe. Results from the UFIREG Study. Am J Respir Crit Care Med.194(10):1233-1241. IF 13.204
2. Adaptation of the human population to the environment: Current knowledge, clues from Czech cytogenetic and "omics" biomonitoring studies and possible mechanisms
The main aim of this review was to provide a comprehensive overview of the last decade of Czech biomonitoring research, concerning the effect of various levels of air pollution and radiation, on the differently exposed population groups. A detailed analysis of data leads to a hypothesis of the versatile mechanism of adaptation to environmental stressors via DNA methylation settings which may even originate in prenatal development, and help to reduce the resulting DNA damage levels.
A model of epigenetic mechanism providing the link between changes of environmental factors during life and the levels of DNA damage.
Publikace:
Rössnerová, A., Pokorná, M., Švecová, V., Šrám, R., Topinka, J., Zölzer, F., Rössner, P.: (2017) Adaptation of the human population to the environment: Current knowledge, clues from Czech cytogenetic and "omics" biomonitoring studies and possible mechanisms. Mutation Research-Reviews in Mutation Research. 773: 188-203. IF=5.5. doi: 10.1016/j.mrrev.2017.07.002. Epub 2017 Jul 12. Review.
3. Mesenchymal stem cells have been proved as a promising tool for cell-based therapy of currently uncurable degenerative diseases of the retina
Degenerative diseases of the retina represent the most common cause of decreased quality of vision or even blindness. So far, there is no treatment protocols for these disorders. We showed that mesenchymal stem cells can be differentiated into cells expressing markers of the retinal cells, possess potent immunoregulatory properties and produce numerous neuroprotective and growth factors. These properties make them a prospective cell type for the treatment of degenerative retinal disorders.
Differentiation of mesenchymal stem cells into cells expressing retinal markers.
Publication:
Heřmánková B., Kössl J., Javorková E., Boháčová P., Hájková M., Zajícová A., Krulová M., Holáň V.: The identification of interferon-gama as a supportive factor for retinal differentiation of murine mesenchymal stem cells. Stem cells Dev. 26, 1399-1408, 2017. IF 3.562
Holáň V. Heřmánková B., Kössl J.: Perspectives of stem cell-based therapy for age-related retinal degenerative diseases. Cell Transplant. 26, 138-141, 2017. IF 3.006
Important results in 2016
1. Urinary 8-oxo-7,8-dihydro-2‘-deoxyguanosine analysis by an improved ELISA: An inter-laboratory comparison study
We performed an inter-laboratory comparison of 8-oxo-7,8-dihydro-2‘-deoxyguanosine by ELISA using standardized conditions, compared the ELISA results with high-performance liquid chromatography-tandem mass spectrometry and performed identification of compounds that may contribute to the discrepancy between methods. Assay standardization significantly improved inter-laboratory agreement, although some variability caused by aromatic and heterocyclic compounds and saccharides is still observed.
An improved ELISA to assess urinary 8-oxo-7,8-dihydro-2‘-deoxyguanosine (8-oxodG)
Using standardized conditions urinary 8-oxodG levels were analyzed. Assay standardization significantly decreased inter-laboratory variability of the assay.
Publication:
Rössner, P., Orhan, H., Koppen, G., Sakai, K., Santella, R.M., Ambrož, A., Rossnerová, A., Šrám, R.,Ciganek, M., Neca, J., Arzuk, E., Mutlu, N., Cooke, M.S.: (2016) Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine analysis by an improved ELISA: An inter-laboratory comparison study. Free Radic. Biol. Med., 22;95: 169-179. IF 5.784
2. The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells
Cobalt-zinc-ferrite nanoparticles (NPs) encapsulated by amorphous silica were tested to assess their effect on DNA, lipids and proteins in rat mesenchymal stem cells. No significant cytotoxic effects were observed, except for the highest dose of CZF-NPs, that slowed down cell proliferation and induced damage to DNA, lipids and proteins. The results suggest that the silica-coated CZF-NPs, when applied at a non-toxic dose, represent a promising contrast agent for cell labeling for magnetic resonance analysis.
Publication:
Novotná, B., Turnovcová, K., Veverka, P., Rössner, P. Jr., Bagryantsevá, Y., Herynek, V., Zvatora, P., Vosmanská, M., Klementová, M., Syková, E., Jendelová, P.: (2016) The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells. Nanotoxicology, 10(6): 662-670. IF 7.913
3. The effects of cytokines on immunomodulatory properties of mesenchymal stem cells
Immunoregulatory and therapeutic properties of mesenchymal stem cells (MSCs) can be modified by cytokines. We described that the production of immunoregulatory molecules by MSCs depends on their activation and that their secretory profile significantly modulates immunological reactions. A novel mechanism used by MSCs to inhibit production of interleukin-10 by activated B cells was characterized. Thus, MSCs can regulate harmful inflammatory reactions in dependence on the cytokine environment.
Immunoregulatory properties of mesechymal stem cells. Mesenchymal stem cells (MSCs) regulate the reactivity of cells of specific immunity (Th- helper T cell, Tc – cytotoxic T cell, Treg – regulatory T cell, Breg – regulatory B cell) by multiple mechanisms in both positive and negative manner.
Publications:
Heřmánková B., Zajícová A., Javorková E., Chudíčková M., Trošan P., Hájková M., Krulová M., Holáň V.: Suppression of IL-10 production by activated B cells via a cell contact-dependent cyclooxygenase-2 pathway upregulated in IFN-gama-treated mesenchymal stem cells. Immunobiology, 221, 129-136, 2016. IF 2.720
Holáň V., Heřmánková B., Boháčová P., Kössl J., Chudíčková M., Hájková M., Krulová M., Zajícová A., Javorková E.: Distinct immunoregulatory mechanisms in mesenchymal stem cells: Role of the cytokine environment. Stem Cells Rev. Rep. 12, 654-663, 2016. IF 2.967
Important results in 2015
1. Day-to-day variability of toxic events induced by organic compounds bound to size segregated atmospheric aerosol
The temporal variability of size-segregated aerosol mass collected on a daily basis during the winter period in highly polluted districts of Ostrava city as well as toxic effects of organic compounds extracted from each size fraction were quantified. We revealed that upper accumulation mode fraction (0.5 < dae < 1 μm) comprises most of the aerosol mass and contained 44% of total c-PAHs while ultrafine fraction (< 0.17 μm) contained only 11% of c-PAHs. DNA adduct levels and dioxin-like activity strongly correlated with both aerosol mass and c-PAH concentrations suggesting genotoxicity as a major toxic effect of organic compounds bound to size-segregated aerosol.
(a) Sum of c-PAH concentrations in four aerosol fractions per mg of the size fraction mass. c-PAHs include benz[a]anthracene, chrysene, benzo[k]fluoranthene, benzo[a]pyrene, dibenz[a,h]anthracene and indeno[1,2.3-cd]pyrene.
(b) Total DNA adduct levels induced by organic extracts from size-segregated aerosols in an acellular system (calf thymus DNA) per mg of the size fraction mass. A strong positive correlation was found between c-PAH content in different aerosol size fractions and DNA adducts (R: 0.62 – 0.86).
Publication:
Topinka, J., Rossner, P. Jr., Milcová, A., Schmuczerová, J., Pěnčíková, K., Rossnerová, A., Ambrož, A., Štolcpartová, J., Bendl, J., Hovorka, J., Machala, M.: (2015) Day-to-day variability of toxic events induced by organic compounds bound to size segregated atmospheric aerosol. Environ. Pollut. 202: 135-145.
2. Reduced gene expression levels after chronic exposure to high concenrations of air pollutants
We analyzed the effect of air pollution on gene expression in subjects living in Prague and in the polluted Ostrava region. We expected to observe changes in expression of DNA repair genes in subjects from the Ostrava region. Unexpectedly, the changes were found in Prague subjects, particularly in genes associated with immune response and neurodegenerative diseases. The results suggest that chronic exposure to air pollution may result in adaptation of the organism with possible negative health effects.
Pathways associated with air pollution in subjects from Prague and the Ostrava region. The figure shows both the pathways associated with location only and the pathways associated with exposure to air pollution (B[a]P – benzo[a]pyrene, PM2.5 – particulate matter of aerodynamic diameter <2.5 µm).
Publication:
Rossner, Jr. P., Tulupová, E., Rossnerová, A., Líbalová, H., Hoňková, K., Gmuender, H., Pastorková, A., Švecová, V., Topinka, J., Šrám, R.J.: (2015) Reduced gene expression levels after chronic exposure to high concentrations of air pollutants. Mutat Res. 780:60-70.
3. Comparative study on the therapeutic potential of mesenchymal stem cells and tissue-specific limbal stem cells
The ability of mesenchymal stem cels (MSC) and limbal stem cells (LSC) to produce various immunoregulatory molecules and to modulate immune response was compared. The therapeutic potential of these stem cells was evaluated in the model of treatment of chemically damaged ocular surface in the rabbit. It was found that stem cells produce a number of immunoregulatory molecules and that MSC have comparable therapeutic properties as do tissue-specific LSC. These results bring the evidence that MSC can replace tissue-specific LSC in the cases when LSC are absent or difficult to obtain.
Characterization of MSC. The ability of MSC to express genes for immunomodulatory molecules was determined by real-time PCR. The cells were cultured untreated or were stimulated with LPS, IFN-γ with LPS plus IFN-γ
Therapeutic potential of MSC and LSC. Stem cells were transferred using nanofiber scaffold onto damaged ocular surface and their therapeutic potential was evaluated. The figure shows healthy rabbit eye, the eye shortly after chemical damage, damaged eye covered with nanofiber scaffold, untreated damaged eye two weeks after the injury and damaged eye treated with MSC or LSCC nebo LSC.
Publications:
Holáň V., Trošan P., Čejka Č., Javorková E., Zajícová A., Heřmánková B., Chudíčková M, Čejková J.: A comparative study of the therapeutic potential of mesenchymal stem cells and limbal epithelial stem cells for ocular surface reconstruction. Stem Cells Translat. Med. 4, 1052-1063, 2015. IF 5.709
2020–2022 EATRIS-CZ II Czech National Node of European Infrastructure in Translational Medicine (MŠMT, LM2018133)
2021–2023 The impact of metal nanoparticles on properties and functions of stem cells: an in vitro and in vivo study (Czech Science Foundation, 21-17720S)
2016–2019, 2020–2022 NanoEnviCz Research Infrastructure (Ministry of Education, Youth and Sports, LM2018124)
2016–2021 Strategie AV21-QUALITAS research programme „Wellbeing in health and disease“
2018–2021 GASODAM Cellular Response Mechanisms of Organic Matter Genotoxicity in Petrol and Alternative Fuel Emissions on the Human Pulmonary Cell Model (Czech Science Foundation, No. 18-06548Y)
2018–2022 HAIE Healthy Aging in Industrial Environment (European Union - European Structural and Investment Funds, Operational Program Research, Development and Education; Ministry of Education, Youth and Sports, CZ.02.1.01/0.0/0.0/16_019/0000798)
2019–2021 GACR– The use of stem cells for introduction of specific transplantation tolerance (No. 19-02290S)
2019–2022 INTER-EXCELENCE, LTAUSA18110 – Selection of diagnostic markers of the long-term effect of multifunctional peptide agonists and antagonists targeting µ-, δ- and ƙ-opioid receptors – search for new possibilities for treatment of chronic pain
2019–2023 TUBE Transport derived Ultrafines and the Brain Effects (Horizon 2020, No. 814978-2)
2020–2022 ADAIR European infrastructure for translational medicine (EATRIS) From air pollution to brain pollution – novel biomarkers to unravel the link of air pollution and Alzheimer’s disease
2022
Holan V, Echalar B, Palacka K, Kossl J, Bohacova P, Porubská B, Krulova M, Javorkova E, Zajicova A.: (2022) The Inability of Ex Vivo Expanded Mesenchymal Stem/Stromal Cells to Survive in Newborn Mice and to Induce Transplantation Tolerance. Stem Cell Rev Rep. doi: 10.1007/s12015-022-10363-7. Epub ahead of print. PMID: 35288846
2021
Holan V , Echalar B, Palacka K, Kossl J, Bohacova P, Krulova M, Brejchova J, Svoboda P, Zajicova A. (2021): The Altered Migration and Distribution of Systemically Administered Mesenchymal Stem Cells in Morphine-Treated Recipients. Stem Cell Rev Rep. 2021 Feb 13. doi: 10.1007/s12015-021-10126-w. Epub ahead of print. PMID: 33582958
Kossl J , Bohacova P, Hermankova B, Javorkova E, Zajicova A, Holan V. (2021): Antiapoptotic Properties of Mesenchymal Stem Cells in a Mouse Model of Corneal Inflammation. Stem Cells Dev. 2021 Apr;30(8):418-427. doi: 10.1089/scd.2020.0195. Epub 2021 Mar 23. PMID: 33607933
2020
Jirakova, K., Moskvin, M., Machova Urdzikova, L., Rossner, P., Elzeinova, F., Chudickova, M., Jirak, D., Ziolkowska, N., Horak, D., Kubinova, S., Jendelova, P.: (2020) The Negative Effect of Magnetic Nanoparticles with Ascorbic Acid on Peritoneal Macrophages. Neurochem Res. 45(1): 159-170. Epub 2019. doi: 10.1007/s11064-019-02790-9. IF= 2.782
Rossnerova, A., Honkova, K., Pelclova, D., Zdimal, V., Hubacek, J.A., Chvojkova, I., Vrbova, K., Rossner, P., Topinka, J., Vlckova, S., Fenclova, Z., Lischkova, L., Klusackova, P., Schwarz, J., Ondracek, J., Ondrackova, L., Kostejn, M., Klema, J., Dvorackova, S.: (2020) DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles. Int J Mol Sci 31;21(7):2420. doi: 10.3390/ijms21072420. IF= 4.556
Rossner, P ., Libalova, H., Vrbova, K., Cervena, T., Rossnerova, A., Elzeinova, F., Milcova, A., Novakova, Z., Topinka, J.: (2020) Genotoxicant Exposure, Activation of the Aryl Hydrocarbon Receptor, and Lipid Peroxidation in Cultured Human Alveolar Type II A549 Cells. Mutat Res 853:503173. doi: 10.1016/j.mrgentox.2020.503173. IF= 2.506
Novotna, B., Pelclova, D., Rossnerova, A., Zdimal, V., Ondracek, J., Lischkova, L., Vlckova, S., Fenclova, Z., Klusackova, P., Zavodna, T., Topinka, J., Komarc, M., Dvorackova, S., Rossner, P.: (2020) The Genotoxic Effects in the Leukocytes of Workers Handling Nanocomposite Materials. Mutagenesis 12;35(4):331-340. doi:10.1093/mutage/geaa016. IF= 3.379
Ujcikova, H., Cechova, K., Roubalova, L., Brejchova, J., Kaufman J., Holan, V., Svoboda P.: (2020) The High-Resolution Proteomic Analysis of Protein Composition of Rat Spleen Lymphocytes Stimulated by Concanavalin A; A Comparison With Morphine-Treated Cells. J. Neuroimmunol. 341: 577191. doi: 10.1016/j.jneuroim.2020.577191. IF= 3.125
Hyzdalova, M., Prochazkova, J., Strapacova, S., Svrzkova, L., Vacek, O., Fedr, R., Andrysik, Z., Hruba, E., Libalova, H., Klema, J., Topinka, J., Masek, J., Soucek, K., Vondracek, J., Machala, M.: (2020) A Prolonged Exposure of Human Lung Carcinoma Epithelial Cells to Benzo[A]Pyrene Induces P21-Dependent Epithelial-to-Mesenchymal Transition (EMT)-Like Phenotype. Chemosphere. 263: 128126.doi.org/10.1016/j.chemosphere.2020.128126 IF=5.788
2019
Brzicova, T., Sikorova, J., Milcova, A., Vrbova, K., Klema, J. , Pikal, P., Lubovska, Z., Philimonenko, A., Franco, F., Topinka, J., Rossner, P., Jr.: (2019) Nano-TiO2 stability in medium and size as important factors of toxicity in macrophage-like cells. Toxicology in Vitro. 54: 178-188. doi: 10.1016/j.tiv.2018.09.019. IF= 3.067
Rossner, P., Libalova, H., Cervena, T., Vrbova, K., Elzeinova, F., Milcova, A., Rossnerova, A., Novakova, Z., Ciganek, M., Pokorna, M., Ambroz, A., Topinka, J.: (2019) The processes associated with lipid peroxidation in human embryonic lung fibroblasts, treated with polycyclic aromatic hydrocarbons and organic extract from particulate matter. Mutagenesis. 34: 153-164. pii: gez004. doi: 10.1093/mutage/gez004. IF= 2.638
Holan, V., Javorkova, E., Vrbova, K., Vecera, Z., Mikuska, P., Coufalik, P., Kulich, P., Skoupy, R., Machala, M., Zajicova, A., Rossner, P.: (2019) A murine model of the effects of inhaled CuO nanoparticles on cells of innate and adaptive immunity – a kinetic study of a continuous three-month exposure. Nanotoxicology.13(7): 952-963. doi: 10.1080/17435390.2019.1602679. IF= 5.811
Cervena, T., Vrbova, K., Rossnerova, A., Topinka, J., Rossner, P., Jr. : (2019) Short-term and Long-term Exposure of the MucilAir™ Model to Polycyclic Aromatic Hydrocarbons. Altern Lab Anim. 47(1): 9-18. doi: 10.1177/0261192919841484. IF= 3.014
Rossnerova, A ., Pelclova, D., Zdimal, V., Rossner, P, Elzeinova, F., Vrbova, K., Topinka, J., Schwarz, J., Ondracek, J., Kostejn, M., Komarc, M., Vlckova, S., Fenclova, Z., Dvorackova, S.: (2019) The repeated cytogenetic analysis of subjects occupationally exposed to nanoparticles: a pilot study. Mutagenesis. 34(3): 253-363. IF= 2.898
Choi, H., Song, WM., Wang, M., Dostal, M., Pastorkova, A., Libalova, H., Tulupova, E., Rossnerova, A., Rossner, P. Jr., Sram, RJ., Zhang, B. (2019): Benzo[a]pyrene is associated with dysregulated myelo-lymphoid hematopoiesis in asthmatic children. Environ Int. 128: 218-232. doi: 10.1016/j.envint.2019.04.052. IF= 7.943
Novotná, B ., Sikorová, J., Milcová, A., Pechout, M., Dittrich, L., Vojtíšek-Lom, M, Rössner, P., Brzicová, T., Topinka, J.: (2019) The genotoxicity of organic extracts from particulate truck emissions produced at various engine operating modes using diesel or biodiesel (B100) fuel: A pilot study. Mutation Research – Genetic Toxicology. 845: 403034. doi: 10.1016/j.mrgentox.2019.03.007. IF= 2.256
Javorkova, E., Matejckova, N., Zajicova, A., Hermankova, B., Hajkova, M., Bohacova, P., Kossl, J., Krulova, M., Holan, V.: (2019) Immunomodulatory properties of bone marrow mesenchymal stem cells of patients with amyotrophic lateral sclerosis and healthy donors. J. Neuroimnune Pharmacol. 14, 215-225. IF=3,870
Hajkova, M., Jaburek, F., Porubska, B., Bohacova, P., Holan, V., Krulova, M.: (2019) Cyclosporine A promotes the therapeutic effect of mesenchymal stem cells on transplantation reaction. Clin. Sci. (Lond). 133, 2143-2157. IF=5,237
Bohacova, P., Kossl, J., Hajkova, M., Hermankova, B., Javorkova, E., Zajicova, A., Krulova, M., Holan, V: (2019) Interleukin-10 production by B cells is regulated by cytokines, but independently of GATA3 or Foxp3 expression. Cell. Immunol, 347 (2020), 103987. IF=3,291
Hermankova, B., Kossl, J., Bohacova, P., Javorkova, E., Hajkova, M., Krulova, M., Zajicova, A., Holan, V.: (2019 ) The immunoregulatory potential of mesenchymal stem cels in a retinal inflammatory environment. Stem Cell Rev. Rep., 14, 215-225. IF=4,697
2018
Procházková, J., Střapacova, S., Svrzkova, L., Andrysík, Z., Hýžd'alová, M., Hrubá, E., Pěnčíková, K., Líbalová, H., Topinka, J., Kléma, J., Espinosa, J. M., Vondráček, J., Machala, M.: (2018) Adaptive changes in global gene expression profile of lung carcinoma A549 cells acutely exposed to distinct types of AhR ligands. Toxicology Letters. 292: 162-174. doi: 10.1016/j.toxlet.2018.04.024. IF=3.749
Choi, H., Dostál, M., Pastorková, A., Rössner, P., Šrám, R., Ho, S.M.: (2018) Greater susceptibility of girls to airborne Benzo[a]pyrene for obesity-associated childhood asthma. Environment International. 121: 308-316. doi: 10.1016/j.envint.2018.08.061. IF=8.763
Hoňková, K., Rössnerová, A., Pavlíková, J., Švecová, V., Kléma, J., Topinka, J., Milcová, A., Líbalová, H. , Choi, H., Veleminský Jr., M., Šrám, R., Rössner ml., P.: (2018) Gene expression profiling in healthy newborns from diverse localities of the Czech Republic. Environmental and Molecular Mutagenesis. 59 (5): 401-415. doi: 10.1002/em.22184. IF=2.528
Libalova, H ., Costa, P.M., Olsson M., Farcal, L., Ortelli, S., Blosi, M., Topinka, J., Costa, A.L., Fadeel, B.: (2018) Toxicity of surface-modified copper oxide nanoparticles in a mouse macrophage cell line: Interplay of particles, surface coating and particle dissolution. Chemosphere. 196: 482-493. doi: 10.1016/j.chemosphere.2017.12.182. IF=4.208
Libalova, H., Milcova, A., Cervena, T., Vrbova, K., Rossnerova, A., Novakova, Z., Topinka, J., Rossner, P., Jr. : (2018) Kinetics of ROS generation induced by polycyclic aromatic hydrocarbons and organic extracts from ambient air particulate matter in model human lung cell lines. Mutat Res Gen Tox En. 827:50-58. doi: 10.1016/j.mrgentox.2018.01.006. IF=2.133
Holan, V.,
Cechova, K., Zajicova, A., Kossl, J., Hermankova, B., Bohacova, P., Hajkova, M., Krulova, M., Svoboda, P., Javorkova, E.: (2018) The impact of morphine on the characteristics and function properties of human mesenchymal stem cells. Stem Cell Rev. Rep. 14, 801-811. IF=3,612
Matejckova, N., Zajicova, A., Hermankova, B., Kossl, J., Bohacova, P., Holan, V., Javorkova, E.: (2018) Characterization of mesenchymal stem cells from patients with amyotrophic lateral sclerosis. J. Clin. Pathol., 71(8), 735-742. IF=2,894
Cechova, K., Hlouskova, M., Javorkova, E., Roubalova, L., Ujcikova, H., Holan, V., Svoboda, P.: (2018) Up-regulation of µ-, κ- and δ–opioid receptors in concanavalin A-stimulated rat spleen lymphocytes. J. Neuroimmunol. 321, 12-23. IF=2,655
Javorkova, E.,
Vackova, J., Hajkova, M., Hermankova, B., Zajicova, A., Holan, V., Krulova, M.: (2018) The effect of clinically relevant doses of immunosuppressive drugs on human mesenchymal stem cells. Biomed. Pharmacother. 97, 402-411. IF=3,457
2017
Červená, T., Rössnerová, A., Sikorová, J. , Beránek, V., Vojtíšek-Lom, M., Ciganek, M., Topinka, J., Rössner, P. Jr.: (2017) DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells. Basic & Clinical Pharmacology & Toxicology. 121 (SI): 102-108. IF= 2.452
Choi, H., Tabashidze, N., Rössner, P., Dostál, M., Pastorková, A., Kong, S.W., Gmuender, H., Šrám, R.: (2017) Altered vulnerability to asthma at various levels of ambient Benzo[a]Pyrene by CTLA4, STAT4 and CYP2E1 polymorphisms. Environmental Pollution. 231: 1134-1144. IF= 6.152
Rössnerová, A., Pokorná, M., Švecová, V., Šrám, R., Topinka, J. , Zölzer, F., Rössner, P.: (2017) Adaptation of the human population to the environment: Current knowledge, clues from Czech cytogenetic and "omics" biomonitoring studies and possible mechanisms. Mutation Research-Reviews in Mutation Research. 773: 188-203. IF=6.081
Urbancová, K., Lanková, D., Rössner ml., P., Rössnerová, A., Švecová, V., Tomaniová, M., Veleminský Jr., M., Šrám, R., Hajslová, J., Pulkrabová, J.: (2017) Evaluation of 11 polycyclic aromatic hydrocarbon metabolites in urine of Czech mothers and newborns. Science of the Total Environment. 577: 212-219. IF=5.727
Hajkova, M., Hermankova B., Javorkova, E., Bohacova, P., Zajicova, A., Holan, V., Krulova, M. : (2017) Mesenchymal stem cells attenuate adverse effects of immunosuppressive drugs on distinct T cell populations. Stem Cells Rev. Rep. 13, 104-115. IF=2,967
Hajkova, M., Javorkova, E., Zajicova, A., Trosan, P., Holan, V., Krulova, M.: (2017) A local application of MSCs and Cyclosporine A attenuates immune response by a switch in macrophage phenotype. J. Tissue Eng. Regen. Med. 11, 1456-1465. IF=3,989
Chudick ova, M ., Bruza, P., Zajicova, A., Trosan, P., Svobodova, L., Javorkova, E., Kubinova, K., Holan, V.: (2017) Targeted neural differentiation of murine mesenchymal stem cells by a protocol simulating the inflammatory site of neural injury. J. Tissue Eng. Regen. Med. 11,1588-1597. IF=3,989
Sirc, J., Hampejsova, Z., Trnovska, J., Kozlik, P, Hrib, J., Hobzova, R., Zajicova, A., Holan, V., Bosakova, Z.: (2017) Cyclosporine A loaded electrospun poly(D,L-lactic acid)/poly(ethylene glycol) nanofibers: Drug carriers utilizable in local immunosupression. Pharm. Res. 34, 1391-1401. IF=3,002
Hermankova, B., Kossl, J., Javorkova, E., Bohacova, P., Hajkova, M., Zajicova, A., Krulova, M., Holan, V.: (2 017) The identification of interferon-γ as a supportive factor for retinal differentiation of murine mesenchymal stem cells. Stem Cells Dev. 26, 1399-1408. IF=3,562
2016
Šrám, R.J., Rössner, P., Rössnerová, A., Dostál, M., Milcová, A., Švecová, V. , Pulkrabová, J., Hajšlová, J., Velemínský, M.: (2016) Impact of Air Pollution to Genome of Newborns. Am. J. Respir. Crit. Care Med., 24: S40-S44. IF=15.196
Veleminský, M., Ambrož, A., Rössner, P., Rössnerová, A., Švecová, V., Milcová, A., Dostál, M., Pastorková, A., Pulkrabová, J., Hajslová, J., Solanský, I., Hanzl, M., Šrám, R.: (2016) Oxidative stress in newborns by different modes of delivery. Neuroendocrinology Letters. 37(6): 445-451. IF=0.918
Ambrož, A., Vlková, V., Rössner, P. Jr., Rössnerová, A., Švecová, V., Milcová, A. , Pulkrabová, J., Hajslová, J., Veleminský, M. Jr., Solanský, I., Šrám, R.: (2016) Impact of air pollution on oxidative DNA damage and lipid peroxidation in mothers and their newborns. Int. J. Hyg. Environ. Health., 219 (6): 545-556.
Lanzinger, S., Schneider A., Breitner, S., Stafoggia, M., Erzen, I., Dostál, M., Pastorková, A., Bastian, S., Cyrys, J., Zscheppang. A., Kolodnitska, T., Peters, A.: (2016) Ultrafine and Fine Particles and Hospital Admissions in Central Europe. Results from the UFIREG Study. American Journal of Respiratory and Critical Care Medicine. 194(10): 1233-1241. IF= 15.196
Andersen, Z. J., Šrám, R. J., Ščasný, M., Gurzau, E. S., Fucic, A., Gribaldo, L., Rossner, P. Jr., Rossnerová, A., Braun Kohlová, M., Máca, V., Zvěřinová, I., Gajdosová, D., Moshammer, H., Rudnai, P., Knudsen, L. E.: (2016) Newborns health in the Danube Region: Environment, biomonitoring, interventions and economic benefits in a large prospective birth cohort study. Environ. Int., 88: 112-122.
Ghosh, R., Rossner, P., Hoňková, K., Dostál, M., Šrám, R., Hertz-Picciotto, I.: (2016) Air pollution and childhood bronchitis: Interaction with xenobiotic, immune regulatory and DNA repair genes. Environ. Int., 87: 94-100.
Lanzinger, S., Schneider, A., Breitner, S., Stafoggia, M., Erzen, I., Dostál, M., Pastorková, A., Bastian, S., Cyrys, J., Zscheppang, A., Kolodnitská, T., Peters, A.: (2016) Associations between ultrafine and fine particles and mortality in five central European cities – Results from the UFIREG study. Environ. Int., 88: 44-52.
Novotná, B., Turnovcová, K. , Veverka, P., Rössner, P. Jr., Bagryantsevá, Y., Herynek, V., Zvatora, P., Vosmanská, M., Klementová, M., Syková, E., Jendelová, P.: (2016) The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells. Nanotoxicology, 10(6): 662-670.
Pulkrabová, J., Stupak, M., Švarcová, A., Rössner, P., Rössnerová, A., Ambrož, A., Šrám, R., Hajslová, J.: (2016) Relationship between atmospheric pollution in the residential area and concentrations of polycyclic aromatic hydrocarbons (PAHs) in human breast milk. Sci. Total Environ., 562: 640-647.
Rössner, P., Orhan, H., Koppen, G., Sakai, K., Santella, R.M., Ambrož, A., Rossnerová, A., Šrám, R., Ciganek, M., Neca, J., Arzuk, E., Mutlu, N., Cooke, M.S.: (2016) Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine analysis by an improved ELISA: An inter-laboratory comparison study. Free Radic. Biol. Med., 22;95: 169-179.
Rössnerová, A., Hoňková, K., Pavlíková, J. , Freitinger-Skalická, Z., Havránková, R., Solanský, I., Rössner, P. Jr., Šrám, R.J., Zölzer, F.: (2016) Mapping the factors affecting the frequency and types of micronuclei in an elderly population from Southern Bohemia. Mutat. Res. -Fundam. Mol. Mech. Mutagen., 793–794: 32-40.
Šrám, R.J., Švecová, V., Rössnerová, A.: (2016) Systematic review of the use of the lymphocyte cytokinesis-block micronucleus assay to measure DNA damage induced by exposure to polycyclic aromatic hydrocarbons. Mutat. Res. -Fundam. Mol. Mech. Mutagen., 770(Pt A):162-169.
Urbancová, K., Lanková, D., Rössner, P., Rössnerová, A., Švecová, V., Tomaniová, M., Veleminský, M. Jr., Šrám, R.J., Hajslová, J., Pulkrabová, J.: (2016) Evaluation of 11 polycyclic aromatic hydrocarbonmetabolites in urine of Czech mothers and newborns. Sci. Total Environ., 212-219.
Andersen, Z. J., Šrám, R. J., Ščasný, M., Gurzau, E. S., Fucic, A., Gribaldo, L., Rossner, P. Jr., Rossnerová, A., Braun Kohlová, M., Máca, V., Zvěřinová, I., Gajdosová, D., Moshammer, H., Rudnai, P., Knudsen, L. E.: (2016) Newborns health in the Danube Region: Environment, biomonitoring, interventions and economic benefits in a large prospective birth cohort study. Environ. Int., 88: 112-122.
Hermankova, B., Zajicova, A., Javorkova, E., Chudickova, M., Trosan, P., Hajkova, M., Krulova, M., Holan, V.: (2016) Suppression of IL-10 production by activated B cells via a cell contact-dependent cyclooxygenase-2 pathway upregulated in IFN-γ-treated mesenchymal stem cells. Immunobiology 221, 129-136. IF=2,781
Trosan, P., Javorkova, E., Zajicova, A., Hajkova, M., Hermankova, B., Kossl, J., Krulova, M., Holan, V.: (2016) The supportive role of insulin-like growth factor-I in the differentiation of murine mesenchymal stem cells into corneal-like cells. Stem Cells Dev. 25, 874-881. IF=3,777
Hermankova, B., Holan, V.: ( 2016) Perspectives of the cell therapy in ophthalmology. 2. The potential of stem cells for the retinal diseases treatment. Cesk. Slov. Oftalmol. 72, 272-275.
Holan, V., Hermankova, B., Bohacova, P., Kossl, J., Chudickova, M., Hajkova, M., Krulova, M., Zajicova, A., Javorkova, E.: (2016) Distinct immunoregulatory mechanisms in mesenchymal stem cells: Role of the cytokine environment. Stem Cels Rev. Rep. 12, 654-663. IF=3,111
2015
2014
Líbalová, H., Krčková, S., Uhlířová, K., Milcová, A., Schmuczerová, J., Ciganek, M., Kléma, J., Machala, M., Šrám, R., Topinka, J.: (2014) Genotoxicity but not the AhR-mediated activity of PAHs is inhibited by other components of complex mixtures of ambient air pollutants. Toxicol Lett. 225(3): 350-357.
Líbalová H., Krčková, S., Uhlířová, K., Klema, J., Ciganek, M., Rössner, P. Jr., Šrám, R., Vondráček, J., Machala, M., Topinka, J.: (2014) Analysis of gene expression changes in A549 cells induced by organic compounds from respirable air particles. Mutat. Res. -Fundam. Mol. Mech. Mutagen. 770: 94-105.
Rössner, P., Jr, Rössnerová, A., Beskid, O., Tabashidze, N., Líbalová, H., Uhlířová, K., Topinka, J., Šrám, R.: (2014) Nonhomologous DNA end joining and chromosome aberrations in human embryonic lung fibroblasts treated with environmental pollutants. Mutat Res. (763-764): 28-38.
Zablotskii, V., Lunov, O., Novotná, B., Churpita, O., Trošan, P., Holáň, V., Syková, E., Dejneka, A., Kubinová, Š.: (2014) Down-regulation of adipogenesis of mesenchymal stem cells by oscillating high-gradient magnetic fields and mechanical vibration. Appl. Phys. Lett. 105(10): 103702
Koci, Z., Turnovcova, K., Dubsky, M., Baranovicova, L., Holan, V., Chudickova, M., Sykova, E., Kubinova, S.: (2014) Characterization of human adipose tissue-derived stromal cells isolated from diabetic patients´s distal limbs with critical limb ischemia. Cell. Biochem. Funct. 32, 597-604.
Zablotskii, V., Lunov, O., Novotna, B., Churpita, O., Trosan, P., Holan, V., Sykova, E., Dejneka, A., Kubinova, S.: (2014) Down-regulation of adipogenesis of mesenchymal stem cells by oscillating high-gradient magnetic fields and mechanical vibration. Appl. Phys. Lett. 105, 103702.
2013
Hyánková, L., Novotná, B.: (2013) Divergent selection for shape of growth curve in Japanese quail: 7. Effect of egg storage at high temperature on embryo development and hatchability. Br. Poult. Sci. 54(6): 695-703.
Rössner, P. Jr., Mistry, V., Singh, R., Šrám, R., Cooke M. S.: (2013) Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS. Biochem. Biophys. Res. Commun. 440(4): 725-730.
Rössner, P. Jr., Švecová, V., Schmuczerová, J., Milcová, A., Tabashidze, N., Topinka, J., Pastorková, A., Šrám, R.: (2013) Analysis of biomarkers in a Czech population exposed to heavy air pollution. Part I. Bulky DNA adducts. Mutagenesis. 28(1): 89-95.
Rössner, P. Jr., Rössnerová, A., Špátová, M., Beskid, O., Uhlířová, K., Líbalová, H., Solanský, I., Topinka, J., Šrám, R.: (2013) Analysis of biomarkers in a Czech population exposed to heavy air pollution. Part II. Chromosomal aberrations and oxidative stress. Mutagenesis. 28(1): 97-106.
Rössnerová, A., Tulupová, E., Tabashidze, N., Schmuczerová, J., Dostál, M., Rössner, P. Jr., Gmuender, H., Šrám, R.: (2013) Factors affecting the 27K DNA methylation pattern in asthmatic and healthy children from locations with various environments. Mutat. Res. -Fundam. Mol. Mech. Mutagen. 741-742: 18-26.
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J. Heyrovský Institute of Physical Chemistry
Palacký University Olomouc
Technical University of Liberec
Jan Evangelista Purkyně University in Ústí nad Labem
Institute of Inorganic Chemistry
Veterinary Research Institute
Institute of Analytical Chemistry
Charles University in Prague
Institute of Chemical Process Fundamentals
Institute of Animal Physiology and Genetics
University of South Bohemia in České Budějovice
University of Chemistry and Technology, Prague
Research Centre for Toxic Compounds in the Environment, Brno
Institute of Macromolecular Chemistry, CAS
Institute of Physiology, CAS
Charles University, Faculty of Science, Prague
Institute for Clinical and Experimental Medicine, Prague
Charles University, First faculty of Medicine and General University Hospital in Prague
The National Institute of Occupational Health, Norway
Norwegian Institute for Air Research, Norway
Karolinska Institutet, Sweden
Institute of Occupational Medicine, Scotland
University of Manchester, UK
Flemish Institute for Technological Research, Belgium
University of Oslo, Norway
King’s College London, UK
Florida International University, MI, USA
University of Aberdeen Scotland
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