Location: Microscopy Service Centre, Institute of Experimental medicine CAS, Vídeňská 1083
Price: 1000 CZK
Contact person: Barbora Hyklová
The course will cover theoretical and practical aspects of an "entry-level" super-resolution imaging methods based on the principle of Optical-Photon-Reassignment available in our facility at confocal microscopes Zeiss LSM880 with AiryScan and Olympus SpinSR10 with OSR.
What is meant by the “entry-level super-resolution microscopy”?
The Entry-Level Super-Resolution microscopy stands for group of methods, which provide resolution improvement by factor of ~2 over the diffraction limited imaging and are very easy to use.
While these methods cannot offer the resolution improvement of factor 5x-10x like STED or STROM/PALM, they are fast, easy to operate and require no special sample preparation or extra skills. They are also very suitable for live cell-imaging.
Main advantages of entry level super-resolution microscopy:
Introduction SR microscopy, explanation of AiryScan and SD-OPR (Spinning Disc Optical Photon Reassignment) principles, facility tour, demonstration of Zeiss LSM 880 AiryScan and Olympus SpinSR10.
Practical hands-on imaging in two parallel groups.
We are open to your own experiments and your samples are welcomed.