Institute of Experimental Medicine CAS

Zeiss LSM 880 Airyscan

Inverted confocal microscope with one-photon excitation.  

Zeiss LSM 880 Airyscan   Zeiss LSM 880 Airyscan   Zeiss LSM 880 Airyscan

 Applications:

  • Fast and multi-color confocal imaging
  • Various measurement options: z-stack, time series, tile scan, multi positions and regions
  • Spectral detection, spectral unmixing, FRAP
  • Super-resolution microscopy with AiryScan detector
  • Long term live-cell imaging available

 

Location: Building La, 3.08b

Contact: Barbora Hyklová, 2718

Training: (odkaz na info o školení)


Downloads:

  • imageJ macro for auto tiff export
  • Manual  (pdf)
  • Full manual (pdf)

Useful links:

 Specifications:

Illumination

Halogen lamp (HAL 100) for transmission light illumination

Fluorescence light source 100W mercury lamp

Laser 405nm (35mW)

Laser 458nm (25mW)

Laser 488nm (25mW)

Laser 514nm (25mW)

Laser 561nm (20mW)

Laser 633nm (5mW)

Objectives

10X, NA 0.45

20X, NA 0.8

40X, NA 1.2, water

63X, NA 1.4, oil

100X, NA 1.46, oil

Filter sets

CFP; GFP; RFP; YFP; DAPI; Cy5

Detectors

2x confocal PMT detector

1x 32 channel spectral confocal GaAsP detector

1x AiryScan detector

1x Transmitted light detector (T-PMT)

Scanning module

2 galvanometer scanning mirrors

Resolution 512x512 pixels, 10 frames/s

Resolution up to 8000x8000 pixels

Stage

PC-controlled motorized stage

Software

ZEISS ZEN Black Edition

Additional equipment

Temperature and CO2 controlled incubated enclosure, DIC prism set