Femtonics FEMTO3D Atlas
Upright two-photon microscope designed for in vivo imaging of small rodents
Applications
- Ultra-fast In vivo imaging.
- Various measurement options: z-stack, time series.
- Simultaneous 2P imaging and 2P photo-stimulation in 3D.
- Incubation chamber for ex vivo measurements.
Location: building La, room 1.15b
Contact: Štěpán Kortus, 2725
Links
Official manual (pdf)
Specifications
|
Illumination |
Fluorescence light source 100W mercury lamp Femtosecond IR Laser 1040nm (10W) for 2P excitation Tuneable IR laser 700-1300nm (3.2W) for 2P excitation LED 475 nm for full-field photo stimulation |
|
Objectives |
Equipped: 4X, 0.4, Air 16X, NA 0.8, WD 3mm, water dipping 25X, NA 1.1, WD 2mm, water dipping/water immersion
Optionally: 20x, NA 0.95, WD 2mm, water dipping 25x, NA 1.05, WD 4mm, water dipping/water immersion |
|
Filter sets |
dapi; GFP; RFP; Triple set |
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Detectors |
2x NDD GaAsP detector Camera |
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Scanning module |
1x galvanometer scanning mirrors Resolution 512x512 pixels, 4frames/s Resolution up to 8000x8000 pixels
1x AOD ultrafast 3D scanner Resolution 512x512 pixels, 40 frames/s Up to kHz scanning speed for special scanning modes |
|
Stage |
PC-controlled motorized stage |
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Software |
Matlab MES |
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Additional equipment |
Temperature and humidity-controlled chamber. Anaesthesia (isoflurane) |
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