Olympus FV10i
Small inverted semi-automatical confocal microscope for fixed samples.
Application
- Routine confocal mikroscopy (z-stack, time-scan)
- Semi-automatic scanning
Location: La building, 3.1
Contact: Barbora Hyklová, 2718
Downloads
Official Olympus Manual (pdf)
Specification
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Illumination |
4 diode lasers: Laser 405nm (22mW) Laser 473nm (15mW) Laser 559nm (18mW) Laser 635nm (12mW) |
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Objectives |
UPLSAPO 10x phase contrast objective, NA 0.4, dry UPLSAPO 60× phase contrast oil-immersion objective, NA 1.35 Zoom magnification can be changed continually from 10x to 600x. |
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Filter sets |
Grating-based emission wavelength selection. Spectral detection system allows to select any wavelength width of the emission spectra in accordance with the used fluorescent dye (no physical filter sets used). |
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Detectors |
2x channel confocal PMT detector, 1x Phase contrast channel Single motorized pinhole, diameter ø50-800μm with automatic setting |
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Scanning module |
2 galvanometer scanning mirrors Pixel size: 256 × 256 - 1024 × 1024 Scanning speed: 1.1 s / frame (for pixel size 512 × 512, High Speed scanning mode) |
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Stage |
Motorized XYZ stage Dedicated specimen holder for glass slides, well slides and glass bottom dish with 35mm diameter
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Software |
Olympus FluoView control software. Map image, one shot, time-lapse (XYT), Z-stack (XYZ), Z-stack time-lapse (XYZT), multi area time-lapse (Multi Area XYT), multi area Z-stack time-lapse (Multi Area XYZT) |
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Additional equipment |
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