Ústav experimentální medicíny AV ČR, v. v. i.

Olympus FV1200 MP

Olympus FV1200 MPE Olympus FV1200 MPE Olympus FV1200 MPE

 Upright fluorescent confocal microscope with single-photon and two-photon excitation. 




  • Confocal and two-photons imaging.
  • Various measurement options: z-stack, time series, tile scan, multi positions and regions.
  • 2 independent scanners for simultaneous imaging and photomanipulations.
  • Spectral detection, thick sample imaging, long term live-cell imaging.


Location:  building La, room 3.1

Contact:: Barbora Hyklová, 2718






Halogen lamp for transmission light illumination

Fluorescence light source 100W mercury lamp

Solid-state laser 405 nm (56 mW)

Solid-state laser 559 nm (100 mW)

Solid-state laser 635 nm (23 mW)

Gas laser (Ar) 488/515 nm (22mW)

Multiphoton laser MaiTai DeepSee 690 – 1040 nm, pulse width <100 fs, avg. power >2.1 W, repetition rate 80 MHz


UPLSAPO10X2 Plan S.Apochromat, NA 0.4, WD 3.1 mm

UPLSAPO20X U Plan S Apo, NA 0.75, WD 0.6 mm

UPLSAPO 60X oil, NA 1.35, WD 0.15 mm

UPLSAPO 100X oil, NA 1.4, WD 0.13 mm

LUMPLFLN 40X water dipping, NA 0,8, WD 3.3 mm

LUMPLFLN 60X water dipping, NA 1, WD 2 mm

XLPLN 25x water dipping/immersion, correction collar, NA 1, WD 4 mm

Filter sets

For fluorescence observation:

U-MWU2 ex.: 330 – 385 nm, em.: LP 420 nm, DM: 400 nm

U-MNB2 ex.: 470 – 490 nm, em.: LP 520 nm, DM: 500 nm

U-MWG2 ex.: 510 – 550 nm, em.: LP 590 nm, DM: 570 nm


3x confocal detectors (2x Hamamatsu photomultiplier R7862 with spectral detection + 1x Hamamatsu photomultiplier R7862 with filter defined detection, single pinhole 50 – 300 µm, 5 µm step)

4x detectors for MP excitation imaging (2x GaAsP-PMT + 2x RXD (highly sensitive PMT)

Transmitted light detector (Hamamatsu PMT R7400)

Scanning module

Main scanner for visible lasers:

Point optical scanning system with 2 galvo-meter mirrors

Secondary SIM scanner for infrared laser:

Multi photon simultaneous imaging and laser stimulation


Max. resolution: 4096 x 4096 px

Scanning speed: 2 – 200 µs/px, 9 steps

Zoom factor: 1 – 50x, 0.5 step


PC-controlled motorized stage


Olympus FluoView control software

Additional equipment

Okolab microscope incubator with adjustable temperature and CO2/O2 concentration, DIC prism set