Ústav experimentální medicíny AV ČR, v. v. i.

Illumination

Halogen lamp for transmission light illumination

Fluorescence light source 100W mercury lamp

Solid-state laser 405 nm (56 mW)

Solid-state laser 559 nm (100 mW)

Solid-state laser 635 nm (23 mW)

Gas laser (Ar) 488/515 nm (22mW)

Multiphoton laser MaiTai DeepSee 690 – 1040 nm, pulse width <100 fs, avg. power >2.1 W, repetition rate 80 MHz

Objectives

UPLSAPO10X2 Plan S.Apochromat, NA 0.4, WD 3.1 mm

UPLSAPO20X U Plan S Apo, NA 0.75, WD 0.6 mm

UPLSAPO 60X oil, NA 1.35, WD 0.15 mm

UPLSAPO 100X oil, NA 1.4, WD 0.13 mm

LUMPLFLN 40X water dipping, NA 0,8, WD 3.3 mm

LUMPLFLN 60X water dipping, NA 1, WD 2 mm

XLPLN 25x water dipping/immersion, correction collar, NA 1, WD 4 mm

Filter sets

For fluorescence observation:

U-MWU2 ex.: 330 – 385 nm, em.: LP 420 nm, DM: 400 nm

U-MNB2 ex.: 470 – 490 nm, em.: LP 520 nm, DM: 500 nm

U-MWG2 ex.: 510 – 550 nm, em.: LP 590 nm, DM: 570 nm

Detectors

3x confocal detectors (2x Hamamatsu photomultiplier R7862 with spectral detection + 1x Hamamatsu photomultiplier R7862 with filter defined detection, single pinhole 50 – 300 µm, 5 µm step)

4x detectors for MP excitation imaging (2x GaAsP-PMT + 2x RXD (highly sensitive PMT)

Transmitted light detector (Hamamatsu PMT R7400)

Scanning module

Main scanner for visible lasers:

Point optical scanning system with 2 galvo-meter mirrors

Secondary SIM scanner for infrared laser:

Multi photon simultaneous imaging and laser stimulation

Max. resolution: 4096 x 4096 px

Scanning speed: 2 – 200 µs/px, 9 steps

Zoom factor: 1 – 50x, 0.5 step

Stage

PC-controlled motorized stage

Software

Olympus FluoView control software

Additional equipment

Okolab microscope incubator with adjustable temperature and CO₂/O₂ concentration, DIC prism set