Ústav experimentální medicíny AV ČR, v. v. i.

Olympus SpinSR10

Olympus SpinSR10 Olympus SpinSR10

Inverted fluorescence confocal spinning disc microscope with super-resolution mode.




  • Fast confocal microscopy with spinning disc technique
  • Z-stack, time series, positions, mosaic
  • Super-resolution mode (resolution up to 120 nm)
  • Advanced automatized experiments

"The authors acknowledge the assistance provided by the Research Infrastructure NanoEnviCz, supported by the Ministry of Education, Youth and Sports of the Czech Republic under Project No. LM2018124."


Location: La building, 1.17

Contact: Barbora Hyklová, 2718






Halogen lamp for transmission light illumination

CoolLED pE-300ultra fluorescence light source (3x LED: 405, 488, 561 nm)

Solid-state laser 405 nm (50 mW)

Solid-state laser 488 nm (100 mW)

Solid-state laser 561 nm (100 mW)


UPLXAPO 10X, dry, NA 0.4, WD 3.1 mm

UPLXAPO 20X, dry, NA 0.8, WD 0.6 mm

UPLXAPO 40X, dry, NA 0.95, WD 0.18 mm

UPLXAPO 60 X, oil, NA 1.42, WD 0.15 mm

Filter sets

DAPI, ex. 405 (DAPI)

FITC, ex. 488 (GFP)

TRITC, ex. 561 (RFP)

Alexa Fluor 640


2x Hamamatsu ORCA-Flash 4.0 CMOS camera for two – channel imaging.

Resolution: 2048 x 2048 px

Pixel size: 6.5 µm x 6.5 µm

Live frame rates: 100 fps (2048 x 2048), binning 2 x 2 and 4 x 4

Scanning module

Yokogawa CSU-W1 confocal scanner unit with spinning disk

Pinhole diameter: 50 µm

Tunable rotation speed >4000 rpm.,

Scan speed >100 fps

Effective view field min F18


Motorized magnification lens for super-resolution mode. XY resolution max 120 nm.


PC-controlled motorized stage


cellSens Dimension, ScanR Acquisition, ScanR Analysis

Additional equipment

PECON cellVivo incubator with adjustable temperature and CO2/O2 concentration