Ústav experimentální medicíny AV ČR, v. v. i.

Illumination

Halogen lamp

Objectives

10x/0,3 EC Plan-neofluar

20x/0,8 Plan-apochromat

40x/1,3 Plan-apochromat Oil Immersion

63x/1,4 Plan-apochromat Oil Immersion

100x/1,4 Plan-apochromat Oil Immersion

63x/1,3 Plan-neofluar Water/Glycerine (adjustable immersion)

Filter sets

DAPI ex.: 335 - 383 nm, em.: 420 - 470 nm, DM: 395 nm

GFP ex.: 450 - 490 nm, em.: 500 - 550 nm, DM: 495 nm

AF546 ex.: 540 - 552 nm, em.: LP 590 nm, DM: 580 nm

CY5 ex.: 590 - 650, em.: LP 665, DM 660 nm

Detectors

Axiocam 305 Color Camera

Sensor: SONY IMX 264 Exmor Pregins Global Shutter CMOS (2465 x 2056 px)

Pixel size: 3,45 x 3,45 µm

Chip size: 8,5 x 7,1 mm

Spectral range: 400 – 720 nm

Live frame rates: 2464 x 2056 – 36 fps, 1232 x 1028 – 88 fps (2x2 binning)

Stage

Non-motorized stage

Software

ZEN blue control software

Additional equipment

DIC prism set